Tannic acid reduced apparent protein digestibility and induced oxidative stress and inflammatory response without altering growth performance and ruminal microbiota diversity of Xiangdong black goats

Abstract

The present study was performed to evaluate the impacts of tannic acid (TA) supplementation at different levels on the growth performance, physiological, oxidative and immunological metrics, and ruminal microflora of Xiangdong black goats. Twenty-four goats were randomly assigned to four dietary treatments: the control (CON, basal diet), the low-dose TA group [TAL, 0.3 % of dry matter (DM)], the mid-dose TA group (TAM, 0.6 % of DM), and the high-dose TA group (TAH, 0.9 % of DM). Results showed that the growth performance was unaffected (P > 0.05) by adding TA, whilst the 0.3 % and 0.6 % TA supplementation significantly decreased (P < 0.05) the apparent digestibility of crude protein (CP) and ruminal NH3-N concentration, and raised (P < 0.05) the level of total volatile fatty acid (TVFA) in rumen. The increments of alanine aminotransferase (ALT), triglyceride (TG), cortisol (CORT), total antioxidant capacity (T-AOC), interleukin (IL)-1β, IL-6, and serumamyloid A (SAA), and decrements of globulin (GLB), immunoglobulin G (IgG), cholinesterase (CHE), glutathione reductase (GR), creatinine (CRE), growth hormone (GH), high-density lipoprotein cholesterol (HDLC), and insulin-like growth factor 1 (IGF-1) to different extents by TA addition were observed. Although the Alpha and Beta diversity of rumen bacterial community remained unchanged by supplementing TA, the relative abundance of the predominant genus Prevotella_1 was significantly enriched (P < 0.05) in TAL. It could hence be concluded that the TA supplementation in the present trial generally decreased CP digestion and caused oxidative stress and inflammatory response without influencing growth performance and ruminal microbiota diversity. More research is needed to explore the premium dosage and mechanisms of effects for TA addition in the diet of goats.

Evaluation of stirring time through a rumen simulation technique: Influences on rumen fermentation and bacterial community

“…In the rumen liquid obtained from the RUSITEC system of this trial, the bacterial community at the phylum level was successively dominated by Proteobacteria, Bacteroidota, and Firmicutes, which further confirmed the predominance of these three bacterial phyla in the ruminal microbiome reported in precedent investigations including our prior studies ( Rey et al, 2014 ; Wang et al, 2017a , 2020 , 2021 , 2022 ; Zhong et al, 2018 ). Besides, the genera Rikenellaceae_RC9_gut_group , Succinivibrionaceae_UCG_002 , and Ruminobacter , and the species Ruminobacter amylophilus were, respectively, identified as the prominent ruminal bacterial taxa in this experiment, and this result was also supported by the findings of previous researches ( Cui et al, 2021 ; Daghio et al, 2021 ; Wu et al, 2021 ).…”

“…In the rumen liquid obtained from the RUSITEC system of this trial, the bacterial community at the phylum level was successively dominated by Proteobacteria, Bacteroidota, and Firmicutes, which further confirmed the predominance of these three bacterial phyla in the ruminal microbiome reported in precedent investigations including our prior studies (Rey et al, 2014;Wang et al, 2017aWang et al, , 2020Wang et al, , 2021Wang et al, , 2022Zhong et al, 2018). Besides, the genera Rikenellaceae_ RC9_gut_group, Succinivibrionaceae_UCG_002, and Ruminobacter,…”

Preliminary Results on the Effects of Soybean Isoflavones on Growth Performance and Ruminal Microbiota in Fattening Goats

“…The ruminal microbiota plays a crucial function in promoting nutrient digestion and utilization, which are closely associated with growth performance and production efficiency [31]. A previous study demonstrated that several species from Prevotella play an important role in protein fermentation [32]. Thus, we further investigated the influence of SIFs on ruminal microbiota using 16S rRNA gene sequencing technology.…”

Nutrient utilization, growth performance, and antioxidative status of Barki lambs fed diets supplemented with black (Nigella sativa) and rocket (Eruca sativa) seeds

“…In addition, the reduction in fiber digestibility could be explained by the high polyphenols content especially tannin, which may bind the bacterial enzymes, prevent the microbial attachment to dietary fibers, or form complexes with cellulose, hemicellulose and pectin (Frutos et al, 2004 ). Furthermore, it was indicated that although high tannin content in feeds that bind with protein can increase the amount of rumen undegradable protein, it may also restrain the synthesis of microbial protein in rumen (Wang et al, 2022 ). Wanapat et al ( 2008 ); Odhaib et al 2018a ); Kholif et al ( 2021 ) suggested that the reducing effect of the medicinal plant’s mixture on digestibility compared with the sole supplement may be ascribed to a synergistic effect or a dose-dependent of these supplements.…”