Tanshinone I exerts cardiovascular protective effects in vivo and in vitro through inhibiting necroptosis via Akt/Nrf2 signaling pathway

Zhuo, Youqiong; Yuan, Renyikun; Chen, Xinxin; He, Jiawei; Chen, Yangling; Zhang, Chenwei; Sun, Kaili; Yang, Shilin; Liu, Zhenjie; Gao, Hongwei

doi:10.1186/s13020-021-00458-7

Cited by 28 publications

(20 citation statements)

References 68 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Unsupervised learning algorithm has excellent feature learning ability, which is conducive to data classification [ 10 ]. Some studies pointed out that, in the process of unsupervised initialization, the introduction of greedy layer-by-layer unsupervised training method can improve the generalization performance of big data [ 11 ]. However, unsupervised deep learning is limited by computer hardware in the process of big data training, resulting in slow convergence [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Diagnostic Value of SonoVue Contrast-Enhanced Ultrasonography in Nipple Discharge Based on Artificial Intelligence

Guo

2021

Journal of Healthcare Engineering

View full text Add to dashboard Cite

This paper aims to explore the application value of SonoVue contrast-enhanced ultrasonography based on deep unsupervised learning (DNS) in the diagnosis of nipple discharge. In this paper, a new model (ODNS) is proposed based on the unsupervised learning model and stack self-coding network. The ultrasonic images of 1,725 patients with breast lesions in the shared database are used as the test data of the model. The differences in accuracy (Acc), recall (RE), sensitivity (Sen), and running time between the two models before and after optimization and other algorithms are compared. A total of 48 female patients with nipple discharge are enrolled. The differences in SE, specificity (SP), positive predictive value (PPV), and negative predictive value (NPV) of conventional ultrasound and contrast-enhanced ultrasonography are analyzed based on pathological examination results. The results showed that when the number of network layers is 5, the classification accuracies of DNS and ODNS model data reached the highest values, which were 91.45% and 98.64%, respectively.

show abstract

Section: Introductionmentioning

confidence: 99%

Diagnostic Value of SonoVue Contrast-Enhanced Ultrasonography in Nipple Discharge Based on Artificial Intelligence

Guo

2021

Journal of Healthcare Engineering

View full text Add to dashboard Cite

show abstract

“…Evidences showed that the accumulation of ROS leads to increased cell mobility, remodeled the cytoskeleton, diminished cell–cell conjunctions and increased the EMT markers through NF-κB, HIF-1, p38 MAPK and PI3K/Akt pathways [ 40 – 42 ]. And the antioxidant agents such as NAC and MPG could attenuate EMT progression through decreasing the production of ROS [ 43 , 44 ]. Therefore, therapeutic targets on ROS could prevent the EMT induced cancer metastasis.…”

Section: Discussionmentioning

confidence: 99%

Cucurbitacin B inhibits TGF-β1-induced epithelial–mesenchymal transition (EMT) in NSCLC through regulating ROS and PI3K/Akt/mTOR pathways

et al. 2022

Self Cite

View full text Add to dashboard Cite

Background Lung cancer is the leading cause of cancer mortality worldwide, and most of the patients after treatment with EGF-TKIs develop drug resistance, which is closely correlated with EMT. Cucurbitacin B (CuB) is a natural product of the Chinese herb Cucurbitaceae plant, which has a favorable role in anti-inflammation and anti-cancer activities. However, the effect of CuB on EMT is still far from fully explored. In this study, the inhibition effect of CuB on EMT was investigated. Methods In this study, TGF-β1 was used to induce EMT in A549 cells. MTS assay was used to detect the cell viability of CuB co-treated with TGF-β1. Wound healing assay and transwell assay were used to determine the migration and invasion capacity of cells. Flow cytometry and fluorescence microscope were used to detect the ROS level in cells. Western blotting assay and immunofluorescence assay were used to detect the proteins expression. Gefitinib was used to establish EGF-TKI resistant NSCLC cells. B16-F10 intravenous injection mice model was used to evaluate the effect of CuB on lung cancer metastasis in vivo. Caliper IVIS Lumina and HE staining were used to detect the lung cancer metastasis of mice. Results In this study, the results indicated that CuB inhibited TGF-β1-induced EMT in A549 cells through reversing the cell morphology changes of EMT, increasing the protein expression of E-cadherin, decreasing the proteins expression of N-cadherin and Vimentin, suppressing the migration and invasion ability. CuB also decreased the ROS production and p-PI3K, p-Akt and p-mTOR expression in TGF-β1-induced EMT in A549 cells. Furthermore, Gefitinib resistant A549 cells (A549-GR) were well established, which has the EMT characteristics, and CuB could inhibit the EMT in A549-GR cells through ROS and PI3K/Akt/mTOR pathways. In vivo study showed that CuB inhibited the lung cancer metastasis effectively through intratracheal administration. Conclusion CuB inhibits EMT in TGF-β1-induced A549 cells and Gefitinib resistant A549 cells through decreasing ROS production and PI3K/Akt/mTOR signaling pathway. In vivo study validated that CuB inhibits lung cancer metastasis in mice. The study may be supporting CuB as a promising therapeutic agent for NSCLC and Gefitinib resistant NSCLC.

show abstract

“…Multiple signaling pathways are regulated by Tan-I, among which nuclear factor erythroid 2 related factor 2 (Nrf2) signaling was identified as an important target of Tan-I [ 25 – 27 ]. Nrf2 inhibits inflammation, oxidative stress and protects mitochondria function in chronic kidney diseases [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

“…Nrf2 inhibits inflammation, oxidative stress and protects mitochondria function in chronic kidney diseases [ 28 ]. Tan-I can directly activate Nrf2 as its potential agonist or indirectly activate Nrf2 through the Akt signaling pathway [ 25 – 27 ]. Thus, whether Tan-I inhibits renal interstitial fibrosis through Nrf2 warrants further study.…”

Section: Discussionmentioning

confidence: 99%

Tanshinone I attenuates fibrosis in fibrotic kidneys through down-regulation of inhibin beta-A

Yang

Huang

et al. 2022

BMC Complement Med Ther

View full text Add to dashboard Cite

Background Tanshinone I (Tan-I), an ingredient of Salvia miltiorrhiza, displays protective effects in several disease models. We aim to study the effect of Tan-I on renal fibrosis and explore its underlining mechanism. Methods Rat renal fibroblasts (NRK-49F) were used as an in vitro model to study the effect of Tan-I. Mouse renal fibrosis model was induced by unilateral ureteral obstruction (UUO) or peritoneally injection of aristolochic acid I (AAI). Results We found that Tan-I dose-dependently inhibited the expression of pro-fibrotic markers in rat renal fibroblasts. Masson staining and Western blotting analysis showed that Tan-I treatment attenuated renal fibrosis in UUO or AAI induced fibrotic kidneys. RNA sequencing analysis identified inhibin beta-A (INHBA), a ligand of TGF-β superfamily, as a downstream target of Tan-I in fibrotic kidneys, which were further verified by qPCR. Western blotting analysis showed that INHBA is up-regulated in UUO or AAI induced fibrotic kidneys and Tan-I reduced the expression of INHBA in fibrotic kidneys. Inhibition of INHBA by Tan-I was further confirmed in rat fibroblasts. Moreover, knockdown of INHBA reduced the expression of pro-fibrotic markers and abolished the ani-fibrotic effect of Tan-I in rat renal fibroblasts. Conclusions We conclude that Tan-I attenuates fibrosis in fibrotic kidneys through inhibition of INHBA.

show abstract

Tanshinone I exerts cardiovascular protective effects in vivo and in vitro through inhibiting necroptosis via Akt/Nrf2 signaling pathway

Cited by 28 publications

References 68 publications

Diagnostic Value of SonoVue Contrast-Enhanced Ultrasonography in Nipple Discharge Based on Artificial Intelligence

Diagnostic Value of SonoVue Contrast-Enhanced Ultrasonography in Nipple Discharge Based on Artificial Intelligence

Cucurbitacin B inhibits TGF-β1-induced epithelial–mesenchymal transition (EMT) in NSCLC through regulating ROS and PI3K/Akt/mTOR pathways

Tanshinone I attenuates fibrosis in fibrotic kidneys through down-regulation of inhibin beta-A

Contact Info

Product

Resources

About