Tanshinone IIA Delivery Silk Fibroin Scaffolds Significantly Enhance Articular Cartilage Defect Repairing <i>via</i> Promoting Cartilage Regeneration

Chen, Wei; Xu, Yong; Li, Hao; Dai, Yao; Zhou, Guangdong; Zhou, Zheng; Xia, Huitang; Liu, Hairong

doi:10.1021/acsami.0c03822

Cited by 42 publications

(45 citation statements)

References 55 publications

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“…All animal experiments in this study were approved by the Animal Care and Ethics Committee of Hunan Academy of Chinese Medicine. Rabbit articular chondrocytes were isolated and cultured according to our previous publication [ 33 ]. In brief, articular cartilage was isolated from the knee joints of New Zealand white rabbits (1 week old) and cut into small pieces (<1 mm 3 ).…”

Section: Methodsmentioning

confidence: 99%

Quercetin modified electrospun PHBV fibrous scaffold enhances cartilage regeneration

Chen

Huang

et al. 2021

J Mater Sci: Mater Med

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It suggests that the poly (3-hydroxybutyric acid-co-3-hydroxyvaleric acid) (PHBV) scaffold can be used for cartilage tissue engineering, but PHBV is short of bioactivity that is required for cartilage regeneration. To fabricate a bioactive cartilage tissue engineering scaffold that promotes cartilage regeneration, quercetin (QUE) modified PHBV (PHBV-g-QUE) fibrous scaffolds were prepared by a two-step surface modification method. The PHBV-g-QUE fibrous scaffold facilitates the growth of chondrocytes and maintains chondrocytic phenotype resulting from the upregulation of SOX9, COL II, and ACAN. The PHBV-g-QUE fibrous scaffold inhibited apoptosis of chondrocyte and reduced oxidative stress of chondrocytes by regulating the transcription of related genes. Following PHBV-g-QUE fibrous scaffolds and PHBV fibrous scaffolds with adhered chondrocytes were implanted into nude mice for 4 weeks, it demonstrated that PHBV-g-QUE fibrous scaffolds significantly promoted cartilage regeneration compared with the PHBV fibrous scaffolds. Hence, it suggests that the PHBV-g-QUE fibrous scaffold can be potentially applied in the clinical treatment of cartilage defects in the future.

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Section: Methodsmentioning

confidence: 99%

Quercetin modified electrospun PHBV fibrous scaffold enhances cartilage regeneration

Chen

Huang

et al. 2021

J Mater Sci: Mater Med

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“…Human articular cartilage ECM + AD-MSCs/chondrocytes [27] HA/collagen/fibrinogen + SMSCs + rhTG-4 [28] Human placenta + BM-MSCs ± PRP [30] Alginate spheres + chondrocytes/chondrons [36] 02 Scaffold-Free Strategies Human freeze-dried cancellous bone + human chondrocyte sheets [43] Human chondrocytes ± human synoviocytes [44] hAMSCs cell sheets + cartilage particles [46] 03 Injectables AD-MSCs ± HA [49] Open-porous PLGA microspheres + BM-MSCs [50] MSCs + chondrocytes [51] PEG/ApoPep-1/TCO + chondrocytes [52] E7-Exo + SF-MSCs + KGN [53] 04…”

Section: Scaffold-based Strategiesmentioning

confidence: 99%

“…In their work, Chen et al (2020) coupled SF scaffolds with TAN and seeded them with New Zealand white rabbit chondrocytes. Assessments of the novel scaffold in vitro and in vivo using nude mice (subcutaneous implantation) and New Zealand white rabbit models with full-thickness cartilage defects revealed that chondrocytes in these scaffolds can produce hyaline-like cartilage and thus promote cartilage regeneration better than SF scaffolds alone, mainly when the concentration of TAN is 10 μg/mL [ 36 ].…”

Section: Tissue Engineering Strategies For Articular Cartilage Regenerationmentioning

confidence: 99%

Current Advances in the Regeneration of Degenerated Articular Cartilage: A Literature Review on Tissue Engineering and Its Recent Clinical Translation

et al. 2021

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Functional ability is the basis of healthy aging. Articular cartilage degeneration is amongst the most prevalent degenerative conditions that cause adverse impacts on the quality of life; moreover, it represents a key predisposing factor to osteoarthritis (OA). Both the poor capacity of articular cartilage for self-repair and the unsatisfactory outcomes of available clinical interventions make innovative tissue engineering a promising therapeutic strategy for articular cartilage repair. Significant progress was made in this field; however, a marked heterogeneity in the applied biomaterials, biofabrication, and assessments is nowadays evident by the huge number of research studies published to date. Accordingly, this literature review assimilates the most recent advances in cell-based and cell-free tissue engineering of articular cartilage and also focuses on the assessments performed via various in vitro studies, ex vivo models, preclinical in vivo animal models, and clinical studies in order to provide a broad overview of the latest findings and clinical translation in the context of degenerated articular cartilage and OA.

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“…Importantly, STS prevented articular cartilage degradation by inhibiting apoptosis and in ammatory cytokine expression levels, suggesting that it might be used to treat osteoarthritis (OA) [9][10][11][12]. Furthermore, STS has been shown to suppress chondrocyte dedifferentiation [13,14].…”

Section: Introductionmentioning

confidence: 99%

STS delivery PCL-MECM based hydrogel hybrid scaffold promote meniscal regeneration through controlling the phenotype of macrophages

Yin

Chen

et al. 2022

Preprint

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BackgroundMeniscus injury has a limited ability to heal itself and often results in the progression to osteoarthritis. Tissue engineering offers the possibility of regeneration of severely damaged meniscus. Immunomodulatory strategies based on biomaterials have a wide range of regenerative potential. Macrophages have a high degree of plasticity and will change in morphology and function under the influence of different tissues. M1 macrophages have pro-inflammatory properties, while M2 macrophages have tissue repair and remodeling functions. It has been reported many times that biological scaffolds induce M2 polarization, resulting in the increase of the M2: M1 macrophage phenotypic ratio, thereby promoting tissue repair. However, there is no report on meniscus repair and regenerative.ResultsIn this study, sodium tanshinone IIA sulfonate (STS) delivery Polycaprolactone (PCL)-meniscus extracellular matrix (MECM) based hydrogel hybrid scaffold was fabricated. PCL provides mechanical support, MECM based hydrogel provides a microenvironment conducive to cell proliferation and differentiation, and STS is used to control the phenotype of macrophages. In vitro experiments, we confirmed that STS could not only transform macrophages from M1 to M2 polarization but also prevent meniscal fibrochondrocytes (MFCs) from oxidative stress, apoptosis, and extracellular matrix degradation induced by inflammatory stimulation, thus having stronger proliferation activity. Results of subcutaneous implantation in vivo showed that hybrid scaffold could induce M2 polarization in the early stage. In addition, a hybrid scaffold seeded with MFCs could achieve good meniscus regeneration and chondroprotective effects in the rabbit.Conclusion STS delivery PCL-MECM based hydrogel hybrid scaffold promotes meniscal regeneration through controlling the phenotype of macrophage, which provides a new direction for tissue engineering meniscus regeneration.

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Tanshinone IIA Delivery Silk Fibroin Scaffolds Significantly Enhance Articular Cartilage Defect Repairing via Promoting Cartilage Regeneration

Cited by 42 publications

References 55 publications

Quercetin modified electrospun PHBV fibrous scaffold enhances cartilage regeneration

Quercetin modified electrospun PHBV fibrous scaffold enhances cartilage regeneration

Current Advances in the Regeneration of Degenerated Articular Cartilage: A Literature Review on Tissue Engineering and Its Recent Clinical Translation

STS delivery PCL-MECM based hydrogel hybrid scaffold promote meniscal regeneration through controlling the phenotype of macrophages

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