Tanshinone IIA improves impaired nerve functions in experimental diabetic rats

Liu, Yanwu; Wang, Lingjuan; Li, Xinkui; Lv, Chen; Feng, Dapeng; Luo, Zhuojing

doi:10.1016/j.bbrc.2010.07.037

Cited by 47 publications

(40 citation statements)

References 25 publications

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“…[27][28][29] Previous studies have demonstrated that TSA can protect the brain from I/R injury. 30,31) However, whether TSA has a neuroprotective effect against apoptosis remains unknown. The present study investigated the neuroprotective effects of TSA.…”

Section: Discussionmentioning

confidence: 99%

Neuroprotective Capabilities of Tanshinone IIA against Cerebral Ischemia/Reperfusion Injury via Anti-apoptotic Pathway in Rats

Chen

et al. 2012

Biological & Pharmaceutical Bulletin

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Danshen, derived from the dried root or rhizome of Salviae miltiorrhizae BGE., has Tanshinone IIA (TSA) as one of its active ingredients. Recent reports have shown that TSA can inhibit the apoptosis induced by serum withdrawal or ethanol in cultured PC12 cells. However, whether TSA has any neuroprotective effect remains unknown. In this study, we investigated the effects of TSA on cerebral apoptosis induced by middle cerebral artery occlusion (MCAO) in which cerebral ischemia had been induced 2 h earlier. Twenty-four hours after reperfusion, the rats were assessed for infarct volume etc. Intraperitoneal administration of 25 and 40 mg/kg TSA 10 min after MCAO significantly diminished infarct volume and brain water content and improved neurological deficits in a dose-dependent manner. The 25 mg/kg dosage was more effective. Treatment with 25 mg/kg TSA significantly improved symptoms and reduce infarct volume at different points in time, of which 10 min after MCAO was the most significant. Nissl-staining and HE-staining of the 25 mg/kg TSA group were more appreciable in terms of improvement relative to the vehicle group in the infarct core. TSA of dosage 25 mg/kg significantly decreased the expression of cleaved caspase-3 protein and increased the expression of B-cell lymphoma 2 (bcl-2) protein in the ischemic cortex. Fewer terminal deoxyribonucleotidyl transferase-mediated deoxyuridine triphosphate biotin nick-end labeling (TUNEL)-positive cells were found in the penumbra of the treated group, but they were significantly more common in the vehicle group. We here conclude that the neuroprotective effects of TSA against focal cerebral ischemic/reperfusion injury are likely to be related to the attenuation of apoptosis.

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Section: Discussionmentioning

confidence: 99%

Neuroprotective Capabilities of Tanshinone IIA against Cerebral Ischemia/Reperfusion Injury via Anti-apoptotic Pathway in Rats

Chen

et al. 2012

Biological & Pharmaceutical Bulletin

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“…Serum glucose was determined with a blood glucose test meter (Roche, Germany) (19). Fasting serum insulin concentrations, estradiol (E 2), luteinizing hormone (LH), follicle-stimulating hormone (FSH), and androstenedione were assessed with Chemiluminescent immunoassay kits (insulin kit, LKIN10310; E 2 kit, LKE210324; LH kit, LKLH10294; FSH kit, LKFS10298; androstenedione kit, LKAO10313; Siemens Medical Solutions Diagnostics, Los Angeles, CA) and requires 50 l serum/assay (20,25).…”

Section: Methodsmentioning

confidence: 99%

Cryptotanshinone reverses reproductive and metabolic disturbances in prenatally androgenized rats via regulation of ovarian signaling mechanisms and androgen synthesis

Yang

Zhang

et al. 2011

American Journal of Physiology-Regulatory, Integrative and Comp

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reverses reproductive and metabolic disturbances in prenatally androgenized rats via regulation of ovarian signaling mechanisms and androgen synthesis. Am J Physiol Regul Integr Comp Physiol 300: R869 -R875, 2011. First published January 12, 2011 doi:10.1152/ajpregu.00334.2010.-This trial explores 1) prenatally androgenized (PNA) rats as a model of polycystic ovary syndrome (PCOS) and 2) reproductive and metabolic effects of cryptotanshinone in PNA ovaries. On days 16 -18 of pregnancy, 10 rats were injected with testosterone propionate (PNA mothers) and 10 with sesame oil (control mothers). At age 3 mo, 12 female offspring from each group were randomly assigned to receive saline and 12 cryptotanshinone treatment during 2 wk. Before treatment, compared with the 24 controls, the 24 PNA rats had 1) disrupted estrous cycles, 2) higher 17-hydroxyprogesterone (P ϭ 0.030), androstenedione (P ϭ 0.016), testosterone and insulin (P values ϭ 0.000), and glucose (P ϭ 0.047) levels, and 3) higher areas under the curve (AUC) for glucose (AUC-Glu, P ϭ 0.025) and homeostatic model assessment for insulin resistance (HOMA-IR, P ϭ 0.008). After treatment, compared with vehicle-treated PNA rats, cryptotanshinone-treated PNA rats had 1) improved estrous cycles (P ϭ 0.045), 2) reduced 17-hydroxyprogesterone (P ϭ 0.041), androstenedione (P ϭ 0.038), testosterone (P ϭ 0.003), glucose (P ϭ 0.036), and insulin (P ϭ 0.041) levels, and 3) lower AUC-Glu (P ϭ 0.045) and HOMA-IR (P ϭ 0.024). Western blot showed that cryptotanshinone reversed the altered protein expressions of insulin receptor substrate-1 and -2, phosphatidylinositol 3-kinase p85␣, glucose transporter-4, ERK-1, and 17␣-hydroxylase within PNA ovaries. We conclude that PNA model rats exhibit reproductive and metabolic phenotypes of human PCOS and that regulation of key molecules in insulin signaling and androgen synthesis within PNA ovaries may explain cryptotanshinone's therapeutic effects.

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“…In line with this evidence, the use of specific cytokine inhibitors, anti-inflammatory cytokines, immunosuppressive agents, and inhibitors of microglial activation have shown antihyperalgesic effects in neuropathic and inflammatory pain animal models [7]. Many extracts isolated from plants such as Lonicera japonica [8] and Olea europea [9] and isolated compounds such as tanchinone IIA [10], miricetin [11], and mangiferin [12] have also been studied for their antihyperalgesic effects. Another plant that could provide valuable effects in ZAP is the species Mangifera indica Linneo (Mangifera indica L., Anacardiaceae family).…”

Section: Introductionmentioning

confidence: 96%

“…The subject was asked to report if the sensation was normal or unpleasant and/or painful. The intensity of allodynia in 2 points of this area was determined according to the Likert scale, and the average of both scores was calculated [10,15]. Equally, the intensity of thermal allodynia was determined; heat was produced by an incandescent lamp (60 watts) placed at a 15-cm distance from the skin over 20 s to provide a warm innocuous stimulus.…”

Section: Methodsmentioning

confidence: 99%

“…In addition, it has been recently demonstrated that this receptor regulates glutamatergic synaptic input to the spinothalamic tract neurons of the spinal cord's deep dorsal horn [14,19]. In these patients, treatment with MSBE cream and tablets decreased the area of dynamic allodynia which is an outcome frequently utilized as an indicator of central sensitization maintained by peripheral input [10,15]. In the formalin test at low concentrations, predominant activity of capsaicin-sensitive neurogenic components is accepted [15,20].…”

Section: Disclosure Statementmentioning

confidence: 99%

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Case Series in Patients with Zoster-Associated Pain Using <i>Mangifera indica </i>L. Extract

Garrido-Suárez

Garrido

Delgado

et al. 2011

Forsch Komplementmed

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Background: Neuroimmune activation has been proposed as a source of new targets for therapeutic intervention in neuropathic pain. Vimang® is an aqueous extract from Mangifera indica L. (common mango) that is traditionally used in Cuba for its antioxidant, anti-inflammatory, analgesic, and immunomodulatory properties. In the present case report, we determine its potential effects in patients with zoster-associated pain. Patients and Methods: 12 patients with zoster-associated pain (6 with subacute herpetic neuralgia and 6 with post-herpetic neuralgia) received a daily dose of 1,800 mg of extract (2 coated 300 mg tablets, 3 times daily before meals) together with low doses of amitriptyline (10–25 mg/day) for 120 days. In addition to the tablets, patients used Vimang® cream 1.2% as a topical agent. The average daily pain score using the Likert scale, area and rate of dynamic allodynia, rate of thermal allodynia, and frequency of burning spontaneous pain were evaluated. Results: Pain scores and sensory abnormalities decreased significantly (p < 0.05) with respect to baseline data from week 4. No adverse events were reported. Conclusion: These results suggest that Vimang® could be beneficial in the treatment of neuropathic pain. However, a controlled clinical trial is necessary to confirm this hypothesis.

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Tanshinone IIA improves impaired nerve functions in experimental diabetic rats

Cited by 47 publications

References 25 publications

Neuroprotective Capabilities of Tanshinone IIA against Cerebral Ischemia/Reperfusion Injury via Anti-apoptotic Pathway in Rats

Neuroprotective Capabilities of Tanshinone IIA against Cerebral Ischemia/Reperfusion Injury via Anti-apoptotic Pathway in Rats

Cryptotanshinone reverses reproductive and metabolic disturbances in prenatally androgenized rats via regulation of ovarian signaling mechanisms and androgen synthesis

Case Series in Patients with Zoster-Associated Pain Using <i>Mangifera indica </i>L. Extract

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