Tanshinone IIA isolated from Salvia miltiorrhiza BUNGE induced apoptosis in HL60 human premyelocytic leukemia cell line

Yoon, Yoosik; Kim, Yeon-Ok; Jeon, Won-Kyung; Park, Hee-Juhn; Sung, Hyun Jea

doi:10.1016/s0378-8741(99)00059-8

Cited by 97 publications

(59 citation statements)

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“…31,32 It significantly improved cell viability, protected effectively primary cultured rat hepatocytes against CCl4-induced injury, inhibited the decrease of SOD activity, 31 demonstrated an obvious protective effect against liver injury induced by CCl4 or D-GalN, decreased ALT and AST in serum and decreased MDA in hepatic homogenate in mice. 32 Previous investigations showed that tanshinone IIA has antioxidant properties 6 and cytotoxic activity against multiple human cancer cell lines; 7 inhibited the proliferation of mouse P388 lymphocytic leukemia cells 8 and human hepatoma cells; [9][10][11][12] induced apoptosis of human hepatocellular carcinoma cells, [10][11][12] human promyelocytic leukemia cells (HL60) and human erythroleukemia cells (K562); 13,14 and induced differentiation of human leukemia cells. [15][16][17] However, to our knowledge, there have been no studies on the anticancer activity of tanshinone IIA in human breast cancer cells in vitro and in vivo except for one report of an anticancer effect on mouse hepatic carcinoma.…”

Section: Discussionmentioning

confidence: 99%

“…7 Studies in vitro have shown that tanshinone IIA inhibited the proliferation of mouse P388 lymphocytic leukemia cells 8 and human hepatoma cells; 9-12 induced apoptosis of human hepatocellular carcinoma cells, 10-12 human promyelocytic leukemic cells (HL60) and human erythroleukemic cells (K562); 13,14 and induced differentiation of human leukemia cells.…”

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confidence: 99%

“…6 The planar phenanthrene ring of the tanshinones may be essential for interaction with DNA molecules, whereas the furano-o-quinone moiety could be responsible for the production of reactive free radicals in the close vicinity of bases that causes DNA damage, giving tanshinone cytotoxic activity against multiple human cancer cell lines. 7 Studies in vitro have shown that tanshinone IIA inhibited the proliferation of mouse P388 lymphocytic leukemia cells 8 and human hepatoma cells; [9][10][11][12] induced apoptosis of human hepatocellular carcinoma cells, [10][11][12] human promyelocytic leukemic cells (HL60) and human erythroleukemic cells (K562); 13,14 and induced differentiation of human leukemia cells. [15][16][17] However, few studies have reported anticancer activity of tanshinone IIA in vivo except for one on mouse hepatic carcinoma.…”

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confidence: 99%

“…19 Many naturally occurring agents have shown cancer chemopreventive potential in a variety of bioassay systems and animal models with relevance to human disease; 20 e.g., genistein, one of the major soy isoflavones, has antioxidant properties, is a potent inhibitor of angiogenesis and metastasis and is a promising reagent for cancer chemoprevention and/or treatment. 21 Based on its antioxidant properties, cytotoxic activity against multiple human cancer cells, induction of apoptosis and differentiation of human hepatocellular carcinoma cells and leukemia cells, [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17] we hypothesized that tanshinone IIA might be a candidate agent for human breast cancer treatment and/or chemoprevention. However there are no such reports.…”

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Potential anticancer activity of tanshinone IIA against human breast cancer

et al. 2005

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Tanshinone IIA is a derivative of phenanthrene-quinone isolated from Danshen, a widely used Chinese herbal medicine. It has antioxidant properties and cytotoxic activity against multiple human cancer cell lines, inducing apoptosis and differentiation of some human cancer cell lines. Our purpose was to confirm its anticancer activity on human breast cancer in vitro and in vivo and to elucidate the mechanism of its activity. Human breast cancer cells were tested in vitro for cytotoxicity, colony formation inhibition, BrdU incorporation and gene expression profiling after treatment with tanshinone IIA. Seven nude mice bearing human breast infiltrating duct carcinoma orthotopically were tested for anticancer activity and expression of caspase-3 in vivo by s.c. injection of tanshinone IIA at a dose of 30 mg/kg 3 times/week for 10 weeks. Tanshinone IIA demonstrated a dose-and time-dependent inhibitory effect on cell growth (IC 50 = 0.25 mg/ml), and it significantly inhibited colony formation and BrdU incorporation of human breast cancer cells. Oligonucleotide microarray analysis identified 41 upregulated (1.22%) and 24 downregulated (0.71%) genes after tanshinone IIA treatment. Upregulated genes were involved predominantly in cycle regulation, cell proliferation, apoptosis, signal transduction and transcriptional regulation; and downregulated genes were associated mainly with apoptosis and extracellular matrix/adhesion molecules. A 44.91% tumor mass volume reduction and significant increase of casepase-3 protein expression were observed in vivo. Our findings suggest that tanshinone IIA might have potential anticancer activity on both ER-positive and -negative breast cancers, which could be attributed in part to its inhibition of proliferation and apoptosis induction of cancer cells through upregulation and downregulation of multiple genes involved in cell cycle regulation, cell proliferation, apoptosis, signal transduction, transcriptional regulation, angiogenesis, invasive potential and metastatic potential of cancer cells. ADPRTL1 might be the main target at which tanshinone IIA acted. ' 2005 Wiley-Liss, Inc.Key words: tanshinone IIA; anticancer activity; breast cancer; growth inhibition; apoptosis Breast cancer is a major public health problem in the United States and in most industrialized countries.1 It is the most common cancer in women and the leading cause of cancer death among women 35-54 years of age.2 The rising incidence and poor prognosis of breast cancer cases have prompted a search for additional preventive and therapeutic modalities.Danshen (Salvia miltiorrhiza Bunge) is a widely used Chinese herbal medicine; its extracts contain diterpene quinone and phenolic acid derivatives, including tanshinone (I, IIA and IIB), cryptotanshinone, isocryptotanshinone, miltirone, tanshinol (I and II) and salviol. These compounds have antioxidant properties and protect against lipid peroxidation in vitro and in vivo, making them potential antidotes for free radical-based disorders.3-5 Tanshinone IIA is a derivative of...

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Potential anticancer activity of tanshinone IIA against human breast cancer

et al. 2005

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“…The study revealed a potential anticancer effect of tanshinone-I on breast cancer cells, suggesting that tanshinone-I may serve as an effective drug for the treatment of breast cancer 116 . Tanshinone II-A, isolated from Salvia miltiorrhiza, induced apoptosis which was linked to proteolytic cleavage of a major component in apoptotic cell death mechanism 117 . Pre application of Zingiber officinale ethanol extract to mouse skin also resulted in a significant inhibition of TPA caused epidermal edema and hyperplasia.…”

Section: Plumbago Zeylanica: Plumbagin Isolated Frommentioning

confidence: 99%

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2017

IJPSR

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Study of tanshinone IIA tissue distribution in rat by liquid chromatography‐tandem mass spectrometry method

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Zhong

et al. 2007

Biomedical Chromatography

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A liquid chromatography/tandem mass spectrometry (LC/MS/MS) method was developed and validated for determining tanshinone IIA in rat tissues. After a single step liquid-liquid extraction with diethyl ether, tanshinone IIA and loratadine (internal standard) was subjected to LC/MS/MS analysis using positive electro-spray ionization under selected reaction monitoring mode. Chromatographic separation of tanshinone IIA and loratadine was achieved on a Hypersil BDS C(18) column (i.d. 2.1 x 50 mm, 5 microm) with a mobile phase consisting of methanol-1% formic acid (90:10, v/v) at a flow rate of 300 microL/min. The intra-day and inter-day precision of the method were less than 10.2 and 12.4%, respectively. The intra-day and inter-day accuracies ranged from 99.7 to 109.7%. The lowest limit of quantification for tanshinone IIA was 1 ng/mL. The method was applied to a tanshinone IIA tissue distribution study after an oral dose of 60 mg/kg to rats. Tanshinone IIA tissue concentrations decreased in the order of stomach > small intestine > lung > liver > fat > muscle > kidneys > spleen > heart > plasma > brain > testes. Tanshinone IIA still could be detected in most of the tissues at 20 h post-dosing. These results indicate that the LC/MS/MS method was rapid and sensitive to quantify tanshinone IIA in different rat tissues.

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Tanshinone IIA isolated from Salvia miltiorrhiza BUNGE induced apoptosis in HL60 human premyelocytic leukemia cell line

Cited by 97 publications

References 12 publications

Potential anticancer activity of tanshinone IIA against human breast cancer

Potential anticancer activity of tanshinone IIA against human breast cancer

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Study of tanshinone IIA tissue distribution in rat by liquid chromatography‐tandem mass spectrometry method

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