Tanycyte-Like Cells Derived From Mouse Embryonic Stem Culture Show Hypothalamic Neural Stem/Progenitor Cell Functions

Kano, Mayuko; Suga, Hidetaka; Ishihara, Takeshi; Sakakibara, Makoto; Soen, Mika; Yamada, Takanobu; Ozaki, Hajime; Mitsumoto, Kazuki; Kasai, Takatoshi; Sugiyama, Mariko; Onoue, Takeshi; Tsunekawa, Taku; Hagiwara, Daisuke; Ito, Yoshihiro; Iwama, Shintaro; Goto, Motomitsu; Banno, Ryoichi; Arima, Hiroshi

doi:10.1210/en.2019-00105

Cited by 18 publications

(14 citation statements)

References 60 publications

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“…In the present work, it was demonstrated that Gap27, a Cx43HC inhibitor, reduced the FGF2‐induced proliferation of β2‐tanycytes, which have been controversially proposed as PNs of the adult hypothalamus (Kano et al., 2019; Lee & Blackshaw, 2012). This proliferative blockade seems to be unique to this cell subtype, since parenchymal cells of hypothalamic neuronal nuclei, ME, and α‐tanycyte cells did not show such dramatic decrease in BrdU incorporation upon FGF2 treatment.…”

Section: Discussionmentioning

confidence: 48%

“…Negative immunoreaction for GFAP in β tanycytes has been recently corroborated by Kano et al. (2019). Since the tanycyte cultures are mainly composed of β‐tanycytes, it is therefore possible to argue that the Cx43‐dependent FGF2‐proliferative response seen here applies to this cell subpopulation, without excluding a possible effect on α‐tanycytes.…”

Section: Resultsmentioning

confidence: 55%

“…FGF2 has been implicated in adult neurogenesis in the classic neurogenic niches such as the subventricular zone (SVZ) and the subgranular zone (SGZ) of the hippocampal dentate gyrus (Woodbury & Ikezu, 2014). Indeed, a combination of FGF2 and EGF, has been widely used for the maintenance and proliferation of neurospheres from different neurogenic niches (Kano et al., 2019; Furube et al, 2020). Extensive in situ evidence informed the expression of FGF2 receptor, FGFR1, in the ventral tanycyte domain (Kaminskas et al., 2019; Samms et al., 2015) and highlighted its importance in the control of body weight and food intake (Samms et al., 2015).…”

Section: Introductionmentioning

confidence: 99%

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The FGF2‐induced tanycyte proliferation involves a connexin 43 hemichannel/purinergic‐dependent pathway

Recabal

Fernández

López

et al. 2020

Journal of Neurochemistry

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In the adult hypothalamus, the neuronal precursor role is attributed to the radial glia‐like cells that line the third‐ventricle (3V) wall called tanycytes. Under nutritional cues, including hypercaloric diets, tanycytes proliferate and differentiate into mature neurons that moderate body weight, suggesting that hypothalamic neurogenesis is an adaptive mechanism in response to metabolic changes. Previous studies have shown that the tanycyte glucosensing mechanism depends on connexin‐43 hemichannels (Cx43 HCs), purine release, and increased intracellular free calcium ion concentration [(Ca2+)i] mediated by purinergic P2Y receptors. Since, Fibroblast Growth Factor 2 (FGF2) causes similar purinergic events in other cell types, we hypothesize that this pathway can be also activated by FGF2 in tanycytes to promote their proliferation. Here, we used bromodeoxyuridine (BrdU) incorporation to evaluate if FGF2‐induced tanycyte cell division is sensitive to Cx43 HC inhibition in vitro and in vivo. Immunocytochemical analyses showed that cultured tanycytes maintain the expression of in situ markers. After FGF2 exposure, tanycytic Cx43 HCs opened, enabling release of ATP to the extracellular milieu. Moreover, application of external ATP was enough to induce their cell division, which could be suppressed by Cx43 HC or P2Y1‐receptor inhibitors. Similarly, in vivo experiments performed on rats by continuous infusion of FGF2 and a Cx43 HC inhibitor into the 3V, demonstrated that FGF2‐induced β‐tanycyte proliferation is sensitive to Cx43 HC blockade. Thus, FGF2 induced Cx43 HC opening, triggered purinergic signaling, and increased β‐tanycytes proliferation, highlighting some of the molecular mechanisms involved in the cell division response of tanycyte. This article has an Editorial Highlight see https://doi.org/10.1111/jnc.15218.

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Section: Discussionmentioning

confidence: 48%

Section: Resultsmentioning

confidence: 55%

Section: Introductionmentioning

confidence: 99%

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The FGF2‐induced tanycyte proliferation involves a connexin 43 hemichannel/purinergic‐dependent pathway

Recabal

Fernández

López

et al. 2020

Journal of Neurochemistry

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Section: G Ener Ation Of Hyp Othal Amic Neur Al S Tem/prog Enitor Cmentioning

confidence: 95%

“…All three neuronal lineages (neurons, astrocytes, and oligodendrocytes) also differentiated from these Rax + neurospheres. Thus, Rax + residual cells in the late stage of hypothalamic differentiation culture were multipotent neural stem/progenitor cells (Kano et al., 2019). These tanycyte‐like neural stem/progenitor cells from pluripotent stem cells could represent a new treatment approach for the dysfunction of hypothalamic energy regulation or homeostatic mechanism caused by disease and aging.…”

Section: Generation Of Hypothalamic Neural Stem/progenitor Cellsmentioning

confidence: 99%

Hypothalamic–pituitary organoid generation through the recapitulation of organogenesis

2021

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The hypothalamic-pituitary system is essential for maintaining physiological homeostasis by controlling systemic hormones, but its regeneration remains mostly unclear. Therefore, attention is focused on the regeneration of the hypothalamus and pituitary from stem cells. Somatic stem cells have drawn attention for their potential for use in regenerative medicine. In the adenohypophysis, the existence of somatic stem cells was reported (Chen et al., 2005), and their functions were discussed at three phases: during early postnatal pituitary maturation (

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Unveiling the Importance of Tanycytes in the Control of the Dialogue Between the Brain and the Periphery

Nampoothiri

Duquenne

Prévot

2021

Masterclass in Neuroendocrinology

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Tanycyte-Like Cells Derived From Mouse Embryonic Stem Culture Show Hypothalamic Neural Stem/Progenitor Cell Functions

Cited by 18 publications

References 60 publications

The FGF2‐induced tanycyte proliferation involves a connexin 43 hemichannel/purinergic‐dependent pathway

The FGF2‐induced tanycyte proliferation involves a connexin 43 hemichannel/purinergic‐dependent pathway

Hypothalamic–pituitary organoid generation through the recapitulation of organogenesis

Unveiling the Importance of Tanycytes in the Control of the Dialogue Between the Brain and the Periphery

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