Tap‐Tagging System in Rice for Protein Complex Isolation

Rohila, Jai S.; Fromm, Michael

doi:10.1002/9780470369630.ch36

Cited by 3 publications

(2 citation statements)

References 25 publications

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“…Among the seven proteins that were identified by MS, dynamin interaction with OsGI was verified with co-immunoprecipitation using a myc-tagged OsGI. This study furthers the use of previously established TAP methodology [43,44] in isolating novel interacting proteins with overexpressed target proteins of interest.…”

Section: Osgi-interactionsupporting

confidence: 63%

Rice proteomics: Ending phase I and the beginning of phase II

2009

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Rice is a critically important food crop plant on our planet. It is also an excellent model plant for cereal crops, and now in position to serve as a reference plant for biofuel production. Proteomics study of rice therefore is crucial to better understand "rice" as a whole. Rice proteomics has moved well beyond the initial proteome analysis in the early to late 1990s. Since the year 2000, numerous proteomic studies have been performed in rice during growth and development and against a wide variety of environmental factors. These proteomic investigations have established the high-resolution 2-D reference gels of rice tissues, organs, and organelle under normal and adverse (stressed) conditions by optimizing suitable, reproducible systems for gel, and MS-based proteomic techniques, which "rejuvenated" the rice proteome field. This constituted the "phase I" in rice proteomics, and resulted in rice being labeled as the "cornerstone" of cereal food crop proteomes. Now, we are in position to state that rice proteomics today marks the "beginning of phase II". This is due to the fact that rice researchers are capable of digging deeper into the rice proteome, mapping PTMs (in particular reversible protein phosphorylation), performing inter- and intra-species comparisons, integrating proteomics data with other "omic" technologies-generated data, and probing the functional aspect of individual proteins. These advancements and their impact on the future of rice proteomics are the focus of this review.

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Section: Osgi-interactionsupporting

confidence: 63%

Rice proteomics: Ending phase I and the beginning of phase II

2009

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“…14‐3‐3 and its client proteins are well studied in plants using the combination of affinity chromatography and proteomics approaches (see review, 59). Tandem affinity purification (TAP)‐tagging system is well established in rice 60 and Arabidopsis 61 to isolate protein complex. In this TAP‐tagging system, isolated protein complex is digested with proteolytic enzyme(s), typically trypsin, and analyzed by MS. Alternatively, protein complex is separated by 1‐DE and excised protein bands are trypsin digested followed by analysis with MS to identify interacting proteins.…”

Section: Technique Of Choice: Classical Protein Extraction and Sepamentioning

confidence: 99%

Rice proteomics: A move toward expanded proteome coverage to comparative and functional proteomics uncovers the mysteries of rice and plant biology

Agrawal¹,

Rakwal

2011

Proteomics

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Growing rice is an important socio-economic activity. Rice proteomics has achieved a tremendous progress in establishing techniques to proteomes of almost all tissues, organs, and organelles during the past one decade (year 2000-2010). We have compiled these progresses time to time over this period. The present compilation discusses proteomics research in rice published between 1st April 2008 and 30th July 2010. Progress continues mainly towards protein cataloging deep into the proteome with high-confident protein assignment and some functional significance than ever before by (i) identifying previously unreported/low-abundance proteins, (ii) quantifying relative/absolute values of proteins, (iii) assigning protein responses to biotic/abiotic stresses, (iv) protein localization into organelles, (v) validating previous proteomes and eliminating false-positive proteins, and (vi) discovering potential biomarkers for tissues, organs, organelles, and for screening transgenic plants and food-safety evaluation. The notable achievements in global mapping of phosphorylation sites and identifying several novel secreted proteins into the extracellular space are worth appreciating. Our ever-increasing knowledge on the rice proteomics is beginning to impact the biology of not only rice, but also crops and plants. These major achievements will be discussed in this review keeping in mind newcomers, young, and established scientists in proteomics and plants.

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Potentials of Proteomics in Crop Breeding

Paudel

Rohila

2015

Advances in Plant Breeding Strategies: Breeding, Biotechnology and Molecular Tools

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Tap‐Tagging System in Rice for Protein Complex Isolation

Cited by 3 publications

References 25 publications

Rice proteomics: Ending phase I and the beginning of phase II

Rice proteomics: Ending phase I and the beginning of phase II

Rice proteomics: A move toward expanded proteome coverage to comparative and functional proteomics uncovers the mysteries of rice and plant biology

Potentials of Proteomics in Crop Breeding

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