Tapachula-7, a New Genetic Sexing Strain of the Mexican Fruit Fly (Diptera: Tephritidae): Sexual Compatibility and Competitiveness

Orozco, Dina; Meza, José S.; Zepeda, Silvia; Solís, Eduardo Salazar; Quintero-Fong, Luis

doi:10.1603/ec12441

Cited by 45 publications

(42 citation statements)

References 42 publications

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“…Over the last two decades, few of them have been available due to genetic instability, poor mating performance, and delayed regulatory approval, for both mass-rearing and field application. There are Ceraitis capitata (Vienna 8) [65], Bactrocera dorsalis (Salaya1) [66], and Anastrepha ludens (Tapachula-7) [67]. Therefore, it is very importance that one strain can be used for controlling other members of the same complex.…”

Section: Discussionmentioning

confidence: 99%

Evidence of weak genetic structure and recent gene flow between Bactrocera dorsalis s.s. and B. papayae, across Southern Thailand and West Malaysia, supporting a single target pest for SIT applications

2014

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BackgroundBactrocera dorsalis s.s. (Hendel) and B. papayae Drew & Hancock, are invasive pests belonging to the B. dorsalis complex. Their species status, based on morphology, is sometimes arguable. Consequently, the existence of cryptic species and/or population isolation may decrease the effectiveness of the sterile insect technique (SIT) due to an unknown degree of sexual isolation between released sterile flies and wild counterparts. To evaluate the genetic relationship and current demography in wild populations for guiding the application of area-wide integrated pest management using SIT, seven microsatellite-derived markers from B. dorsalis s.s. and another five from B. papayae were used for surveying intra- and inter-specific variation, population structure, and recent migration among sympatric and allopatric populations of the two morphological forms across Southern Thailand and West Malaysia.ResultsBasic genetic variations were not significantly different among forms, populations, and geographical areas (P > 0.05). Nonetheless, two sets of microsatellite markers showed significantly different levels of polymorphisms. Genetic differentiation between intra- and inter-specific differences was significant, but low. Seventeen populations revealed three hypothetical genetic clusters (K = 3) regardless of forms and geographical areas. The genetic structure of sympatric populations slightly changed during the different years of collection. Recent gene flow (m ≥ 0.10) was frequently detected whether samples were sympatric or allopatric. Ninety-five of 379 individuals distributed across the given area were designated as recent migrants or of admixed ancestry. As a consequence of substantial migration, no significant correlation between genetic and geographic distances was detected (R2 = 0.056, P = 0.650).ConclusionsAccording to the 12 microsatellite variations, weak population structure and recent gene flow suggest that there is no status for cryptic species between B. dorsalis s.s. and B. papayae forms in Southern Thailand and West Malaysia. Both forms can be treated as a single target pest for the SIT program in an area-wide sense. Additionally, the result of species identification based on molecular data and morphological character are not congruent. The use of independent, multiple approaches in the characterization of the target population may ensure the effectiveness and feasibility of SIT-based control in the target area.

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Section: Discussionmentioning

confidence: 99%

Evidence of weak genetic structure and recent gene flow between Bactrocera dorsalis s.s. and B. papayae, across Southern Thailand and West Malaysia, supporting a single target pest for SIT applications

2014

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“…The strains were produced following the procedures described by Domínguez et al (2010) for the SMR strain and by Orozco et al (2013b) for the Tap-7 strain. Before irradiation, each pupal lot was marked with a different dye color (DayGlo® Color Corp, California, USA; i.e., fire orange and aurora pink for the SMR strain, and signal green and Saturn yellow for the Tap-7 strain; colors were alternated every 14 d) so that the origin of the adults captured in the field could be determined.…”

Section: Biological Materialsmentioning

confidence: 99%

“…After mechanical separation, the Tap-7 males must undergo irradiation and holding for emergence before the release of adults that are chilled at temperatures between 0 and 3 °C for ease of handling . Tests in field cages indicated that Tap-7 sterile males did not differ in time to sexual maturity, sexual compatibility, and sexual competitiveness when compared with sterile males of the bisexual strain and wild males (Orozco et al 2013b).…”

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confidence: 94%

“…In this strain, the males emerge from normal-colored pupae (brown color), whereas the females emerge from black-colored pupae (Zepeda 2010;Orozco et al 2013b). This characteristic allows the sorting of pupae by Sortex Z+-Buhler® machines (Buhler Sortex Inc., Stockton, California, USA), which select for the waste pupae (black in this case) by using an optical system and leave only the desired brown male pupae.…”

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confidence: 99%

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Genetic Consequences of Domestication and Mass Rearing of Pest Fruit Fly <I>Bactrocera tryoni</I> (Diptera: Tephritidae)

Gilchrist¹,

Cameron²,

Sved³

et al. 2012

jnl. econ. entom.

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“…This consistency is attributed to the beginning of ripening and harvest of grapefruits, which also has a positive effect on the mating success in A. ludens males (Robacker & Fraser, 2002;Birke et al, 2006;Morato et al, 2015). Strategic use of the sterile insect technique (SIT) through the release of the genetic sexing strain 'Tapachula 7' (Orozco et al, 2013) offers a viable alternative to interrupt reproductive patterns of A. ludens populations. Strategic use of the sterile insect technique (SIT) through the release of the genetic sexing strain 'Tapachula 7' (Orozco et al, 2013) offers a viable alternative to interrupt reproductive patterns of A. ludens populations.…”

Section: Discussionmentioning

confidence: 99%

Analysis of temporal fluctuations in numbers of sexually mature Anastrepha ludens females over an extensive citrus area in northeastern Mexico

Vanoye‐Eligio

Vázquez‐Sauceda

Rosas‐Mejía

et al. 2019

Entomologia Exp Applicata

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The Mexican fruit fly, Anastrepha ludens Loew (Diptera: Tephritidae), is one of the most important pests in citrus-growing areas in Mexico. In this study, we analyzed the temporal fluctuation of sexually mature females of A. ludens populations with respect to the availability of commercial hosts and climatic factors, as well as population data of females over an extensive citrus area in northeastern Mexico. The numbers of mature females were treated as proportions and analyzed with generalized linear mixed models with a binomial error distribution. Results revealed positive effects on proportions of mature females when commercial hosts (Valencia orange and grapefruit) were available at both medium and high levels, whereas negative effects on proportions were observed when commercial hosts were scarcely available. Monthly analyses indicated positive trends on proportion in March, April, October, and November, whereas negative trends were seen in January and June. A low capture of mature females during the late summer and the onset of winter suggests transitional phenological processes across the year. Overall, no relationships were observed between proportions with temperature and the total number of females; however, a positive relationship was observed between proportion and rainfall. These findings provide insight into the reproductive phenology of A. ludens with practical implications for fruit sampling, as well as for the autocidal or chemical control in an areawide integrated pest management (AW-IPM) approach in citrus-growing areas of northeastern Mexico.

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Tapachula-7, a New Genetic Sexing Strain of the Mexican Fruit Fly (Diptera: Tephritidae): Sexual Compatibility and Competitiveness

Cited by 45 publications

References 42 publications

Evidence of weak genetic structure and recent gene flow between Bactrocera dorsalis s.s. and B. papayae, across Southern Thailand and West Malaysia, supporting a single target pest for SIT applications

Evidence of weak genetic structure and recent gene flow between Bactrocera dorsalis s.s. and B. papayae, across Southern Thailand and West Malaysia, supporting a single target pest for SIT applications

Genetic Consequences of Domestication and Mass Rearing of Pest Fruit Fly <I>Bactrocera tryoni</I> (Diptera: Tephritidae)

Analysis of temporal fluctuations in numbers of sexually mature Anastrepha ludens females over an extensive citrus area in northeastern Mexico

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