Tape transfer sectioning of tissue microarrays introduces nonspecific immunohistochemical staining artifacts

Catchpoole, Daniel; Mackie, Nicole; McIver, S; Chetcuti, Albert; Henwood, Anthony; Graf, Nicole; Arbuckle, Susan

doi:10.3109/10520295.2010.527859

Cited by 9 publications

(6 citation statements)

References 15 publications

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“…Cutting sections from a TMA is technically more challenging than cutting WTS and is prone to specific artefacts, such as variation in the thickness across the section and gross distortion of the section (resulting in loss of orientation of the cores). Special training in cutting TMA sections will reduce tissue loss due to such artefacts, and novel techniques, such as the ‘tape‐transfer’ method, may also reduce sectioning artefacts . Sections should be cut in batches to avoid tissue loss which occurs when refacing the block to cut new sections.…”

Section: Pre‐analytical Considerationsmentioning

confidence: 99%

Guidelines and considerations for conducting experiments using tissue microarrays

et al. 2013

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Tissue microarrays (TMAs) represent a powerful method for undertaking large-scale tissue-based biomarker studies. While TMAs offer several advantages, there are a number of issues specific to their use which need to be considered when employing this method. Given the investment in TMA-based research, guidance on design and execution of experiments will be of benefit and should help researchers new to TMA-based studies to avoid known pitfalls. Furthermore, a consensus on quality standards for TMA-based experiments should improve the robustness and reproducibility of studies, thereby increasing the likelihood of identifying clinically useful biomarkers. In order to address these issues, the National Cancer Research Institute Biomarker and Imaging Clinical Studies Group organized a 1-day TMA workshop held in Nottingham in May 2012. The document herein summarizes the conclusions from the workshop. It includes guidance and considerations on all aspects of TMA-based research, including the pre-analytical stages of experimental design, the analytical stages of data acquisition, and the postanalytical stages of data analysis. A checklist is presented which can be used both for planning a TMA experiment and interpreting the results of such an experiment. For studies of cancer biomarkers, this checklist could be used as a supplement to the REMARK guidelines.

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Section: Pre‐analytical Considerationsmentioning

confidence: 99%

Guidelines and considerations for conducting experiments using tissue microarrays

et al. 2013

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“…The use of an adhesive tape can be helpful to avoid the loss of cores and fall-out in the sectioned TMA. The use of adhesive tape should be employed with caution, since the tape can influence the thickness of sectioned cores and subsequently also the outcome of IHC staining 15 . In the Human Protein Atlas set-up a TMA template of 9x8 (excluding markers) cores are used.…”

Section: Discussionmentioning

confidence: 99%

Production of Tissue Microarrays, Immunohistochemistry Staining and Digitalization Within the Human Protein Atlas

Kampf

Olsson

Ryberg

et al. 2012

JoVE

165

178

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The tissue microarray (TMA) technology provides the means for high-throughput analysis of multiple tissues and cells. The technique is used within the Human Protein Atlas project for global analysis of protein expression patterns in normal human tissues, cancer and cell lines. Here we present the assembly of 1 mm cores, retrieved from microscopically selected representative tissues, into a single recipient TMA block. The number and size of cores in a TMA block can be varied from approximately forty 2 mm cores to hundreds of 0.6 mm cores. The advantage of using TMA technology is that large amount of data can rapidly be obtained using a single immunostaining protocol to avoid experimental variability. Importantly, only limited amount of scarce tissue is needed, which allows for the analysis of large patient cohorts . Approximately 250 consecutive sections (4 μm thick) can be cut from a TMA block and used for immunohistochemical staining to determine specific protein expression patterns for 250 different antibodies. In the Human Protein Atlas project, antibodies are generated towards all human proteins and used to acquire corresponding protein profiles in both normal human tissues from 144 individuals and cancer tissues from 216 different patients, representing the 20 most common forms of human cancer. Immunohistochemically stained TMA sections on glass slides are scanned to create high-resolution images from which pathologists can interpret and annotate the outcome of immunohistochemistry. Images together with corresponding pathology-based annotation data are made publically available for the research community through the Human Protein Atlas portal (www.proteinatlas.org) (Figure 1) 3 4. The Human Protein Atlas provides a map showing the distribution and relative abundance of proteins in the human body. The current version contains over 11 million images with protein expression data for 12.238 unique proteins, corresponding to more than 61% of all proteins encoded by the human genome.

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“…Furthermore, according to Hoos and Cordon-Cardo the lowest degree of tissue damage was seen without using the adhesive transfer tape [19]. Catchpoole et al describe a higher incidence of nonspecific staining in immunohistochemistry by using the tape method [69]. For more details on loss of cores see also the review of Pinder et al [70].…”

Section: The Development Of the Tmas In The Course Of Timementioning

confidence: 99%

Overview on Techniques to Construct Tissue Arrays with Special Emphasis on Tissue Microarrays

Vogel

2014

Microarrays

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With the advent of new histopathological staining techniques (histochemistry, immunohistochemistry, in situ hybridization) and the discovery of thousands of new genes, mRNA, and proteins by molecular biology, the need grew for a technique to compare many different cells or tissues on one slide in a cost effective manner and with the possibility to easily track the identity of each specimen: the tissue array (TA). Basically, a TA consists of at least two different specimens per slide. TAs differ in the kind of specimens, the number of specimens installed, the dimension of the specimens, the arrangement of the specimens, the embedding medium, the technique to prepare the specimens to be installed, and the technique to construct the TA itself. A TA can be constructed by arranging the tissue specimens in a mold and subsequently pouring the mold with the embedding medium of choice. In contrast, preformed so-called recipient blocks consisting of the embedding medium of choice have punched, drilled, or poured holes of different diameters and distances in which the cells or tissue biopsies will be deployed manually, semi-automatically, or automatically. The costs of constructing a TA differ from a few to thousands of Euros depending on the technique/equipment used. Remarkably high quality TAs can be also achieved by low cost techniques.

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Tape transfer sectioning of tissue microarrays introduces nonspecific immunohistochemical staining artifacts

Cited by 9 publications

References 15 publications

Guidelines and considerations for conducting experiments using tissue microarrays

Guidelines and considerations for conducting experiments using tissue microarrays

Production of Tissue Microarrays, Immunohistochemistry Staining and Digitalization Within the Human Protein Atlas

Overview on Techniques to Construct Tissue Arrays with Special Emphasis on Tissue Microarrays

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