TaqMan-Based Real-Time PCR Method for Detection of
            <i>Yersinia pseudotuberculosis</i>
            in Food

Lambertz, S. Thisted; Nilsson, C.; Hallanvuo, Saija

doi:10.1128/aem.01459-08

Cited by 37 publications

(15 citation statements)

References 16 publications

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“…Yersinia enterocolitica was detected with the amplification of the ail gene [ 13 ], using a previously described procedure [ 12 ]. To detect all the Y. pseudotuberculosis serotypes, the wzz and ail genes were amplified in two independent reactions [ 12 , 14 , 15 ]. Amplification of the ail gene detects all serotypes but O:11 and O:12, and amplification of the wzz gene detects all serotypes but O:6 and O:7 [ 14 , 15 ].…”

Section: Methodsmentioning

confidence: 99%

“…To detect all the Y. pseudotuberculosis serotypes, the wzz and ail genes were amplified in two independent reactions [ 12 , 14 , 15 ]. Amplification of the ail gene detects all serotypes but O:11 and O:12, and amplification of the wzz gene detects all serotypes but O:6 and O:7 [ 14 , 15 ]. A sample was considered positive for Y. enterocolitica or Y. pseudotuberculosis when at least one positive result was obtained in the direct reaction or after enrichment in any of the three rt-PCRs used.…”

Section: Methodsmentioning

confidence: 99%

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Prevalence of Yersinia enterocolitica and Yersinia pseudotuberculosis in wild boars in the Basque Country, northern Spain

et al. 2015

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BackgroundYersiniosis is a zoonosis widely distributed in Europe and swine carry different serotypes of Yersinia enterocolitica and Y. pseudotuberculosis. The aim of this study was to determine the prevalence of Y. enterocolitica and Y. pseudotuberculosis in wild boars in northern Spain. The blood of wild boars (n = 505) was sampled between 2001 and 2012. Seroprevalence was determined in 490 serum samples with an indirect enzyme-linked immunosorbent assay. Seventy-two of the animals were also examined for the presence of Y. enterocolitica or Y. pseudotuberculosis in the tonsils with real-time polymerase chain reaction. All the tonsils were analysed twice, directly and after cold enrichment in phosphate-buffered saline supplemented with 1 % mannitol and 0.15 % bile salts.ResultsAntibodies directed against Y. enterocolitica and Y. pseudotuberculosis were detected in 52.5 % of the animals. Yersinia enterocolitica was detected with real-time polymerase chain reaction in 33.3 % of the wild boars and Y. pseudotuberculosis in 25 %. Significant differences were observed according to the sampling year, and the highest prevalence was during winter and spring. The highest antibody levels and Y. enterocolitica prevalence were observed in mountainous areas at altitudes higher than 600 m, with very cold winters, and with the highest annual rainfall for each dominant climate. Areas with low and medium livestock populations were associated with the highest seroprevalence of Yersinia spp. in wild boars, whereas areas with high ovine populations had the highest prevalence of Y. enterocolitica.ConclusionsThis study shows that Y. enterocolitica and Y. pseudotuberculosis are highly prevalent among wild boars in the Basque country, with Y. enterocolitica most prevalent. The risk of infection among wild boars is influenced by the season and the area in which they live.Electronic supplementary materialThe online version of this article (doi:10.1186/s13028-016-0184-9) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Prevalence of Yersinia enterocolitica and Yersinia pseudotuberculosis in wild boars in the Basque Country, northern Spain

et al. 2015

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“…Milk, milk filter and bovine faecal samples were analysed at the Finnish Food Safety Authority Evira. Screening of environmental samples for YP, pathogenic Yersinia enterocolitica and STEC was conducted by real-time PCR [9][10][11]. For Y. enterocolitica detection, we followed the technical specification ISO/WD 10273 [12] and for STEC detection we followed ISO/TS 13136 [11], where presumptive STEC is defined as real-time PCR detection of (i) the stx gene, (ii) stx and eae genes or (iii) stx and eae genes as well as genes associated with one of the serogroups O157, O111, O26, O103 or O145.…”

Section: Traceback and Microbiological Investigationmentioning

confidence: 99%

Outbreak of Yersinia pseudotuberculosis O:1 infection associated with raw milk consumption, Finland, spring 2014

et al. 2015

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In March 2014, a Yersinia pseudotuberculosis (YP) outbreak was detected by a municipal authority in southern Finland. We conducted epidemiological, microbiological and traceback investigations to identify the source. We defined a case as a person with YP infection notified to the National Infectious Disease Registry between February and April 2014, or their household member, with abdominal pain and fever≥38 °C or erythema nodosum. Healthy household members were used as household-matched controls. We identified 43 cases and 50 controls. The illness was strongly associated with the consumption of raw milk from a single producer. The odds ratio of illness increased with the amount of raw milk consumed. Also previously healthy adults became infected by consuming raw milk. Identical YP strains were identified from cases' stool samples, raw milk sampled from a case's refrigerator and from the milk filter at the producer's farm. The producer fulfilled the legal requirements for raw milk production and voluntarily recalled the raw milk and stopped its production. We advised consumers to heat the raw milk to 72 °C for 15 s. Current legislation for raw milk producers should be reviewed and public awareness of health risks linked to raw milk consumption should be increased.

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“…The remaining BHI suspensions were supplemented with 15% glycerol before storage in −80°C for subsequent analysis. Duallabelled probe real-time PCR was performed for Salmonella [14], Y. enterocolitica [15] and Y. pseudotuberculosis [16] on the DNA extracts. Any sample with a threshold cycle (C t ) < 35 was considered positive.…”

Section: Pcr Analysis and Isolation Of Pathogensmentioning

confidence: 99%

Prevalence and genomic characteristics of zoonotic gastro-intestinal pathogens and ESBL/pAmpC producing Enterobacteriaceae among Swedish corvid birds

Söderlund

Skarin

Börjesson

et al. 2019

Infection Ecology & Epidemiology

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Introduction: Wild birds pose a potential threat to animal and human health by spreading infectious diseases. In the present study, we studied the occurrence of bacterial zoonotic pathogens as well as enterobacteria with transferrable antimicrobial resistance genes among Swedish corvids. Materials and methods: Intestines from 66 jackdaws, crows, rooks and magpies from the vicinity of livestock farms at 14 locations in 7 counties were analysed by direct culture or PCR screening followed by culture. Isolates were investigated by whole-genome sequencing. Results and discussion: Campylobacter jejuni were detected in 82% and Yersinia in 3% of the birds. ESBL-producing E. coli were found in one sample (2%) and carried bla CTX-M-55. No Enterobacteriaceae with transferable carbapenem resistance were identified. No Salmonella or E. coli O157:H7 were found, but PCR analysis for enterohaemorrhagic E. coli virulence genes revealed 35% positive samples for intimin, 9% for verotoxin 1 and 17% for verotoxin 2. C. jejuni isolates from corvids were compared to previously published isolates from Swedish sources by multi-locus sequence typing based on genome sequences. All corvid C. jejuni isolates formed a cluster, intermingled with human and chicken isolates. Our results indicate that C. jejuni is ubiquitous among Swedish corvid birds, with sporadic transmission to poultry and humans.

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TaqMan-Based Real-Time PCR Method for Detection of Yersinia pseudotuberculosis in Food

Cited by 37 publications

References 16 publications

Prevalence of Yersinia enterocolitica and Yersinia pseudotuberculosis in wild boars in the Basque Country, northern Spain

Prevalence of Yersinia enterocolitica and Yersinia pseudotuberculosis in wild boars in the Basque Country, northern Spain

Outbreak of Yersinia pseudotuberculosis O:1 infection associated with raw milk consumption, Finland, spring 2014

Prevalence and genomic characteristics of zoonotic gastro-intestinal pathogens and ESBL/pAmpC producing Enterobacteriaceae among Swedish corvid birds

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