Taraxasterol suppresses cell proliferation and boosts cell apoptosis via inhibiting GPD2-mediated glycolysis in gastric cancer

Zhao, Yupei; Zhang, Li; Guo, Min; Yang, Huiling

doi:10.1007/s10616-021-00499-8

Cited by 10 publications

(5 citation statements)

References 26 publications

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“…To investigate the regulatory mechanism of ssc-miR-143-3p in PTr2 cells, a total of 11,406 target genes were predicted using miRanda, RNAhybrid and Targetscan, of which 14 target genes were identified by the intersection of results of the three online tools ( Figure 6 A). Among these 14 target genes, glycerol-3-3 phosphate dehydrogenase 2 ( GDP2 ) was reported playing an indelible role in regulating cell proliferation, migration, apoptosis and cell cycle [ 36 ]. Therefore, we selected GPD2 for subsequent validation.…”

Section: Resultsmentioning

confidence: 99%

“…GPD2 is a protein located in the inner mitochondrial membrane and is mainly associated with glycolysis, inflammatory immune response, cellular senescence and tumorigenesis [ 46 ]. Overwhelming evidence substantiates that GPD2 is involved in regulating cell proliferation, migration and apoptosis [ 36 ]. In this study, three online tools, miRanda, RNAhybrid and Targetscan, were used to analyze GPD2 as a possible target gene of ssc-miR-143-3p, and triple validation by a dual luciferase reporter gene, RT-qPCR, and Western blot further confirmed the existence of targeting relationship between them.…”

Section: Discussionmentioning

confidence: 99%

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Effect of miR-143-3p from Extracellular Vesicles of Porcine Uterine Luminal Fluid on Porcine Trophoblast Cells

Ding

Gan

et al. 2022

Animals

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Extracellular vesicles (EVs) in uterine luminal fluid (ULF) can reportedly affect the proliferation and migration function of porcine trophoblast cells (PTr2 cells) by mediating the maternal–fetal exchange of information. miR-143-3p is considered a crucial miRNA in early pregnancy in mammals; however, little is currently known about how it regulates the function of PTr2 cells. This study aimed to investigate the effects of ssc-miR-143-3p in ULF-EVs on the function of PTr2 cells during porcine embryo implantation. The uptake of ULF-EVs by PTr2 cells was confirmed, which significantly increased the expression of ssc-miR-143-3p. Ssc-miR-143-3p was found to facilitate the proliferation and migration of PTr2 cells in the CCK-8, EdU and wound-closure assays, while the opposite findings were observed after the knockdown of ssc-miR-143-3p. Bioinformatics analysis and the luciferase reporter assay showed that glycerol-3 phosphate dehydrogenase 2 (GDP2) was directly targeted by miR-143-3p. Inhibition of miR-143-3p was validated in mice to inhibit embryo implantation. In summary, ssc-miR-143-3p in ULF-EVs affects the proliferation and migration of PTr2 cells by mediating GPD2, thereby affecting embryo implantation.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Effect of miR-143-3p from Extracellular Vesicles of Porcine Uterine Luminal Fluid on Porcine Trophoblast Cells

Ding

Gan

et al. 2022

Animals

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“…In the cytoplasm, GPD2 can transform the compound dihydroxyacetone phosphate (DHAP) into the enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPD) and NADH. By preferentially converting GAPD to DHAP to catalyze glycolysis, GPD2 activation changes its affinity with substrates and encourages the growth of glioma cells (Lu et al, 2020; Zhao et al, 2021). In the glycolysis process, PGK2 is a crucial protease that can catalyze the formation of ATP as well as the conversion of glycerol-1,3-diphosphate to 3-phosphoglyceric acid (Wu et al, 1997).…”

Section: Discussionmentioning

confidence: 99%

Inhibition of Tumor Glycolysis by Umbelliferone and its Binding to Glycolytic Proteins

Peng

Wang

Xia

et al. 2023

Pharmacognosy Magazine

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Background and Objectives Both primary and secondary cancers require the involvement of glycolytic pathways. Cancer cell proliferation leads to the upregulation of glycolysis, which results in increased glucose consumption. For demonstrating that umbelliferone can effectively bind to several proteins involved in the glycolytic pathway, thereby inhibiting glycolysis and reducing cancer cell proliferation. Materials and Methods This study uses transcriptomics, network pharmacology, and molecular docking to predict the potential targets and possible pathways of umbelliferone against cancer and microscale thermophoresis (MST) to detect the affinity between umbelliferone and potential targets. Results Transcriptomic analysis revealed that differentially expressed genes were primarily associated with glycolytic and other metabolic pathways and proteins. According to network pharmacology and molecular docking results, glycolysis-related proteins such as glucose-6-phosphate isomerase (GPI), glycerol-3-phosphate dehydrogenase, mitochondrial (GPD2), phosphoglycerate kinase 2 (PGK2), and heat shock protein HSP-90 alpha (Hsp90AA1) are potential targets of umbelliferone against tumors. MST confirmed that umbelliferous lactone binds strongly to GPI, GPD2, and PGK2 but not to Hsp90AA1. Conclusion By binding to the glycolysis-related proteins such as GPI, GPD2, and PGK2, umbelliferone acts as an anti-tumor agent by inhibiting glycolysis, cutting off the energy supply to tumor tissue, and reducing tumor growth. It was suggested that umbelliferone might be a brand-new tumor glycolysis inhibitor and that these glycolysis-related proteins might be potential new targets for cancer therapy. This finding helped to establish a solid foundation for the anti-cancer action of umbelliferone.

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“…Cancer cells with knockout of AGL expression were more metabolically active and dependent on glycolysis than cells that express AGL normally ( Lew et al, 2015 ). GPD2 can inhibit glycolysis in tumor cells ( Wu et al, 2020 ; Zhao et al, 2021 ). In our study, the KEGG pathway enrichment analysis results indicated that the 2 most significantly enriched pathways were related to the energy metabolism in muscles (Table S3).…”

Section: Discussionmentioning

confidence: 99%

Identification of the differentially expressed genes in the leg muscles of Zhedong white geese (Anser cygnoides) reared under different photoperiods

HangFeng

et al. 2022

Poultry Science

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Taraxasterol suppresses cell proliferation and boosts cell apoptosis via inhibiting GPD2-mediated glycolysis in gastric cancer

Cited by 10 publications

References 26 publications

Effect of miR-143-3p from Extracellular Vesicles of Porcine Uterine Luminal Fluid on Porcine Trophoblast Cells

Effect of miR-143-3p from Extracellular Vesicles of Porcine Uterine Luminal Fluid on Porcine Trophoblast Cells

Inhibition of Tumor Glycolysis by Umbelliferone and its Binding to Glycolytic Proteins

Identification of the differentially expressed genes in the leg muscles of Zhedong white geese (Anser cygnoides) reared under different photoperiods

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