Tare Soil Disinfestation from Cyst Nematodes Using Inundation

Berger, Beatrice; Daub, Matthias; Gärtner, Kathleen; Becker, Matthias; König, Stephan

doi:10.3390/life13010057

Cited by 4 publications

(3 citation statements)

References 25 publications

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“…Inundation has proven an effective eradication strategy, with loss of viability of PCN cyst content (Runia et al, 2014). Nevertheless, non-viable cysts were still isolated after inundation (Berger et al, 2023;Runia et al, 2014). The use of resistant potato varieties in infested fields has been used with high success to eradicate G. rostochiensis (Price et al, 2021;de Boer et al, 2021;Gartner et al, 2021;Mwangi et al, 2021;Orlando & Boa, 2023).…”

Section: Discussionmentioning

confidence: 99%

Comparing the effectiveness of real-time PCRs to simultaneously detect and identify viable Globodera pallida and G. rostochiensis

Lombard,

Dekker-Nooren,

Wuijster

et al. 2024

Eur J Plant Pathol

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Recently, the Commission Implementation Regulation (CIR) 2022/1192 was published by the European Union, prescribing regulations on eradication measures for quarantine potato cyst nematodes, including mandated assays for species identification. The CIR 2022/1192 provides a derogation on the use of Beniers et al. (2014) real-time PCR as the validation process was still ongoing. In this study, the validation of a modified version of the Beniers real-time PCR was carried out, following EPPO Standard PM 7/98. Furthermore, the performance was compared to the conventional PCR of Bulman & Marshall (1997) and the Gamel et al. (2017) real-time PCR, both DNA based, which are mandated by CIR 2022/1192. Additionally, the effect of cyst viability was compared with the two real-time PCR assays. The modified Beniers real-time PCR met all validation performance criteria for both target Globodera pallida and G. rostochiensis. The viability assays showed that the DNA based Gamel real-time PCR provided positive results for non-viable, non-infectious cysts, in contrast to the modified RNA based Beniers real-time PCR. Based on the results of this study, the modified Beniers qualifies for the detection and identification of viable Globodera pallida and G. rostochiensis and performs at least equally as well as the other two PCR assays. To encourage the development and improvement of diagnostic protocols, it is suggested that diagnostic laboratories performing official surveys are allowed to use internationally recognized diagnostic protocols when validated in-house according to EPPO standards and accredited by the EU bodies (EPPO, 2021).

show abstract

Section: Discussionmentioning

confidence: 99%

Comparing the effectiveness of real-time PCRs to simultaneously detect and identify viable Globodera pallida and G. rostochiensis

Lombard,

Dekker-Nooren,

Wuijster

et al. 2024

Eur J Plant Pathol

View full text Add to dashboard Cite

show abstract

“…Inundation has proven an effective eradication strategy, with loss of viability of PCN cyst content(Runia et al, 2014). Nevertheless, non-viable cysts were still isolated after inundation(Runia et al, 2014;Berger et al, 2023). The use of resistant potato varieties in infested elds has been used with high success to eradicate G. rostochiensis(Price et al., 2020; de Boer et al, 2021; Gartner et al, 2021; Mwangi et al, 2021; Orlando & Boa, 2023).…”

mentioning

confidence: 99%

Comparing the effectiveness of real-time PCRs to simultaneously detect and identify viable Globodera pallida and G. rostochiensis.

Lombard,

Dekker-Nooren,

Wuijster

et al. 2023

Preprint

View full text Add to dashboard Cite

Recently, the Commission Implementation Regulation (CIR) 2022/1192 has been published in Europe, prescribing regulations on eradication measures for quarantine potato cyst nematodes including mandated assays for species identification. The CIR 2022/1192 provides a derogation on the use of Beniers et al. (2014) real-time PCR as the validation process was still ongoing. In this study, the validation of a modified version of the Beniers real-time PCR was carried out, following EPPO Standard PM 7/98. Furthermore, the performance was compared to the conventional PCR of Bulman & Marshall (1997) and the Gamel et al. (2017) real-time PCR, both DNA based, which are mandated by CIR 2022/1192. Additionally, the effect of cyst viability was compared for the two real-time PCR assays. The modified Beniers real-time PCR met all validation performance criteria, with analytical sensitivity of 1 living egg for both target Globodera pallida and G. rostochiensis and scoring 100% for analytical specificity, reproducibility, robustness and diagnostic specificity. The viability assays showed that the DNA based Gamel real-time PCR provided positive results for non-viable cysts in contrast to the modified RNA based Beniers real-time PCR. Based on the results of this study, the modified Beniers qualifies for the detection and identification of Globodera pallida and G. rostochiensis and performs at least equally well as the other two PCR assays. For development and improvement of diagnostic protocols, it is recommended that competent authorities should be allowed to use recognized diagnostic protocols when in-house validated instead of only mandated tests as prescribed by CIR 2022/1192.

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“…The activation of specific microbial activity and the release of other nematicidal compounds may contribute to RKN-soil suppressiveness at the strawberry farms [ 11 ]. Berger and colleagues [ 12 ] found that the inundation process of two types of tare soils (namely sedimentation basins and the Brukner basin) during sugar beet processing efficiently decreased the viability of G. pallida and H. schachtii under laboratory-controlled conditions. These findings offer promising strategies for eco-friendly PPN control in agriculture.…”

mentioning

confidence: 99%