Target‐Cell‐Specific Delivery, Imaging, and Detection of Intracellular MicroRNA with a Multifunctional SnO<sub>2</sub> Nanoprobe

Dong, Haifeng; Lei, Jianping; Ju, Huangxian; Zhi, Feng; Wang, Hua; Guo, Wenjie; Zhu, Zhu; Yan, Feng

doi:10.1002/anie.201108302

Cited by 117 publications

(94 citation statements)

References 55 publications

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“…[ 7 ] In 2012, a multifunctional SnO 2 nanoprobe was reported for monitoring an intracellular miR. [ 8 ] A two-fl uorophore-labeled fl uorescence-resonance energy-transfer nanofl are for the qualitative measurement of mRNAs was designed in 2015, [ 9 ] and a nanoprobe for detecting miRs in differentiating stem cells was also proposed. [ 10 ] However, these organic-dye-based sensing platforms have restricted sensitivity due to the defi ciency of dyes.…”

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confidence: 99%

Gold‐Quantum Dot Core–Satellite Assemblies for Lighting Up MicroRNA In Vitro and In Vivo

Zhao

Sun

et al. 2016

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confidence: 99%

Gold‐Quantum Dot Core–Satellite Assemblies for Lighting Up MicroRNA In Vitro and In Vivo

Zhao

Sun

et al. 2016

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“…Wu et al [22] presented a novel strategy by using PNA probes labeled with fluorophores; they were conjugated with nanometal-organic framework (NMOF) as a fluorescence quencher of the labeled PNA and developed for multiplexed miRNAs detection in living cancer cells. A multifunctional SnO 2 nanoprobe for target-cell-specific delivery and imaging with detection of intracellular miRNA was reported by Ju et al [23] (Fig. 7.4).…”

Section: Functional Nanoprobe-based Intracellular Mirna Detectionmentioning

confidence: 89%

“…The unique thermodynamic and relatively low background of MB attract intensive interest in molecular detection of intracellular targets. Several MB-based signal-on imaging strategies have been constructed to detect endogenous miRNAs [7,23,38,39]. In order to improve the sensitivity, molecular biological amplification technique was used to intracellular miRNA detection.…”

Section: Mirna Imaging Analysismentioning

confidence: 99%

Intracellular and Organic miRNA In Situ Detection

Zhang

Dong

Tian

2015

SpringerBriefs in Molecular Science

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The detection methods of miRNA in intracellular or organisms fall into two broad categories: indirect detection and direct analysis. The indirect measurement of the expression levels of miRNAs in cells and tissues involves cells lysis and detection by qRT-PCR, northern blotting, or microarray hybridization. The direct analysis methods are a noninvasive manner for repetitively monitoring and obtaining real-time imaging of the intracellular miRNA by using imaging analysis or in situ hybridization (ISH). Technologies for direct detection of the temporal and spatial expression sequence of specific miRNA in cells or tissues are extremely important for elucidating miRNA biology. The progress of optical imaging techniques with multimodal reporter systems holds great promise for noninvasive and real-time imaging of molecular agent expression in living cell. Recent progress in nanotechnology and imaging detection techniques leads to multifunctional nanoprobe with specific-transfection, tracing, and regulation function in intracellular miRNA detection. ISH holds great promise for visualization of the spatial localization of RNA at the tissue, cellular, and even subcellular level.Keywords Intracellular miRNA · Organic miRNA · In situ detection · Cell imaging analysis · Functional nanoprobeThe detection of intracellular miRNA is significant in the development of gene therapy and gene medicines. The deficiency of small size and degradation of the mature miRNAs make it difficult to directly transfer the specific miRNA into the cell. A noninvasive manner for repetitively monitoring and obtaining real-time imaging of the intracellular miRNA is required for the analysis of miRNAs in practical clinic application. Efficient gene vectors, including viral [1] and nonviral categories, [2] were usually required to translocate miRNA probe through membrane barrier into the cell. Retrovirus and adenovirus vectors can be effectively transferred via the gene probes (DNA, RNA) into most cell lines. However, the

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“…212 A multifunctional nanoprobe is prepared by functionalizing SnO 2 NPs with both folic acid as targeting moiety and a gene probe to inhibit or recognize intracellular miRNA levels for target cell-specific imaging and in situ detection of intracellular miRNA. 213 A sensitive and selective sensor for detecting colon cancer based on NP covalent modified antihuman epithelial cell adhesion-molecule antibody was also developed. 214 Recently, a highly efficient multifunctional nanoplatform, multicolor luminescent NaYF 4 :Yb 3+ ,Er 3+ upconversion NPs were reported by Liu et al, 215 which could be utilized in image-guided cancer photodynamic therapy.…”

Section: Recent Developments In Nanotechnologybased Cancer Detectionmentioning

confidence: 99%

Nanotechnology-based approaches in anticancer research

Jabir¹,

Tabrez²,

Ashraf³

et al. 2012

IJN

149

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Cancer is a highly complex disease to understand, because it entails multiple cellular physiological systems. The most common cancer treatments are restricted to chemotherapy, radiation and surgery. Moreover, the early recognition and treatment of cancer remains a technological bottleneck. There is an urgent need to develop new and innovative technologies that could help to delineate tumor margins, identify residual tumor cells and micrometastases, and determine whether a tumor has been completely removed or not. Nanotechnology has witnessed significant progress in the past few decades, and its effect is widespread nowadays in every field. Nanoparticles can be modified in numerous ways to prolong circulation, enhance drug localization, increase drug efficacy, and potentially decrease chances of multidrug resistance by the use of nanotechnology. Recently, research in the field of cancer nanotechnology has made remarkable advances. The present review summarizes the application of various nanotechnology-based approaches towards the diagnostics and therapeutics of cancer.

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Target‐Cell‐Specific Delivery, Imaging, and Detection of Intracellular MicroRNA with a Multifunctional SnO₂ Nanoprobe

Cited by 117 publications

References 55 publications

Gold‐Quantum Dot Core–Satellite Assemblies for Lighting Up MicroRNA In Vitro and In Vivo

Gold‐Quantum Dot Core–Satellite Assemblies for Lighting Up MicroRNA In Vitro and In Vivo

Intracellular and Organic miRNA In Situ Detection

Nanotechnology-based approaches in anticancer research

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Target‐Cell‐Specific Delivery, Imaging, and Detection of Intracellular MicroRNA with a Multifunctional SnO2 Nanoprobe

Cited by 117 publications

References 55 publications

Gold‐Quantum Dot Core–Satellite Assemblies for Lighting Up MicroRNA In Vitro and In Vivo

Gold‐Quantum Dot Core–Satellite Assemblies for Lighting Up MicroRNA In Vitro and In Vivo

Intracellular and Organic miRNA In Situ Detection

Nanotechnology-based approaches in anticancer research

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Target‐Cell‐Specific Delivery, Imaging, and Detection of Intracellular MicroRNA with a Multifunctional SnO₂ Nanoprobe