Target delivery of MYCN siRNA by folate-nanoliposomes delivery system in a metastatic neuroblastoma model

Zhu, Qiqi; Chen, Feng; Liao, Wei‐Wei; Zhang, Yan; Tang, Suo-Qin

doi:10.1186/1475-2867-13-65

Cited by 27 publications

(20 citation statements)

References 27 publications

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“…In our study, Oct4‐positive cells were found more frequently in tumors with unfavorable group compared with favorable group and the presence of Oct4‐positive cells was more frequent in higher stages in comparison with lower stages (23 cases vs 4, respectively, although no significant relationship was found). Oct4 is one of the factors, which repress the differentiation process in the blastocytes of preimplanted embryo and is required to activate genes involving in early development . Newton et al.…”

Section: Discussionmentioning

confidence: 99%

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Expression and prognostic significance of Oct4 and Nanog in neuroblastoma

Monajemzadeh

Soleimani

Vasei

et al. 2013

APMIS

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Neuroblastoma is the most common extracranial solid tumor of children, accounting for an estimated 15% cancer-related deaths in this period. It has been hypothesized that drug resistance of cancer stem cells may be responsible for chemotherapy failure, sustained tumor growth, and recurrence in many solid tumors. In this study, we investigated the expression of Octamer-binding transcription factor 4 (Oct4) and Nanog, two stem cell markers, in 47 neuroblastic tumors by immunohistochemistry and correlated their expression by other prognostic factors especially with NMYC amplification using both fluorescent and chromogenic in situ hybridization methods. Twenty three cases (48.9%) showed Oct4 signals and eight cases (17%) showed Nanog expression. All Nanog positive tumors showed Oct4 expression. Seven cases (14.1%) had NMYC amplification. There was also no association between positive Oct4 and Nanog reactivity and tumor morphology, age, mitosis-karyorrhexis index, NMYC amplification, favorable or unfavorable histology, and risk groups (p > 0.05). Cancer stem cells hypothesis is a challenging issue and controversies exist about their significance. Although our study did not show strong association between prognostic factors and expression of stem cell markers, performing of further large-scale studies of various neuroblastic tumors with various stages is suggested.

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Section: Discussionmentioning

confidence: 99%

“…Neuroblastoma (NB) is the most common extracranial solid tumor of children, accounting for an estimated 15% cancer‐related deaths in this period . It is an embryonic tumor that arises from sympathetic nervous system.…”

mentioning

confidence: 99%

Expression and prognostic significance of Oct4 and Nanog in neuroblastoma

Monajemzadeh

Soleimani

Vasei

et al. 2013

APMIS

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“…A modified nanoprecipitation method was used to entrap DM1 into the FA-PLA-TPGS NPs [ 23 , 24 ]. Briefly, a pre-weighed amount of DM1 drug powder and 100 mg of FA-PLA-TPGS copolymer were dissolved in 8 mL of acetone by vortexing and sonication.…”

Section: Methodsmentioning

confidence: 99%

Enhanced anticancer activity of DM1-loaded star-shaped folate-core PLA-TPGS nanoparticles

et al. 2014

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The efficient delivery of therapeutic drugs into interested cells is a critical challenge to broad application of nonviral vector systems. In this research, emtansine (DM1)-loaded star-shaped folate-core polylactide-d-α-tocopheryl polyethylene glycol 1000 succinate (FA-PLA-TPGS-DM1) copolymer which demonstrated superior anticancer activity in vitro/vivo in comparison with linear FA-PLA-TPGS nanoparticles was applied to be a vector of DM1 for FR+ breast cancer therapy. The DM1- or coumarin 6-loaded nanoparticles were fabricated, and then characterized in terms of size, morphology, drug encapsulation efficiency, and in vitro drug release. And the viability of MCF-7/HER2 cells treated with FA-DM1-nanoparticles (NPs) was assessed. Severe combined immunodeficient mice carrying MCF-7/HER2 tumor xenografts were treated in several groups including phosphate-buffered saline control, DM1, DM1-NPs, and FA-DM1-NPs. The antitumor activity was then assessed by survival time and solid tumor volume. All the specimens were prepared for formalin-fixed and paraffin-embedded tissue sections for hematoxylin-eosin staining. The data showed that the FA-DM1-NPs could efficiently deliver DM1 into MCF-7/HER2 cells. The cytotoxicity of DM1 to MCF-7/HER2 cells was significantly increased by FA-DM1-NPs when compared with the control groups. In conclusion, the FA-DM1-NPs offered a considerable potential formulation for FR+ tumor-targeting biotherapy.

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“…Results suggest that PELP1-siRNA-DOPC nanoliposomes can be used as a potential therapeutic modality for the prevention or treatment of ovarian cancer metastasis [105]. Several other siRNA-liposomal formulations targeting key genes involved in cancer growth, proliferation, drug resistance, and metastasis are under investigation [106][107][108][109][110].…”

Section: Nanoliposomes As Delivery Systems Of Sirna and Mirnas For Camentioning

confidence: 99%