Target Format and Hybridization Conditions

Reid, Alex

doi:10.1385/1-59259-238-4:001

Gene Probes

DOI: 10.1385/1-59259-238-4:001

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Target Format and Hybridization Conditions

Alex Reid¹

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Overview of Hybridization and Detection Techniques

Hilario

Protocols for Nucleic Acid Analysis by Nonradioactive Probes

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A misconception regarding the sensitivity of nonradioactive methods for screening genomic DNA libraries often hinders the establishment of these environmentally friendly techniques in molecular biology laboratories. Nonradioactive probes, properly prepared and quantified, can detect DNA target molecules to the femtomole range. However, appropriate hybridization techniques and detection methods should also be adopted for an efficient use of nonradioactive techniques. Detailed descriptions of genomic library handling before and during the nonradioactive hybridization and detection are often omitted from publications. This chapter aims to fill this void by providing a collection of technical tips on hybridization and detection techniques.

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Overview of Hybridization and Detection Techniques

Hilario

Protocols for Nucleic Acid Analysis by Nonradioactive Probes

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show abstract

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Target Format and Hybridization Conditions

Cited by 1 publication

References 2 publications

Overview of Hybridization and Detection Techniques

Overview of Hybridization and Detection Techniques

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