Target grafting of poly(2‐(dimethylamino)ethyl methacrylate) to biodegradable block copolymers

Abstract

A series of copolymers composed of methoxy poly(ethylene glycol) and a hydrophobic block of poly(ɛ‐caprolactone‐co‐propargyl carbonate) grafted with poly(2‐[dimethylamino]ethyl methacrylate) was synthesized by combining ring opening polymerization, azide‐alkyne click reaction, and atom transfer radical polymerization (ATRP). Well‐defined copolymers with a target composition and a tailored structure were achieved via the grafting from approach by using a single catalytic system for both click reaction and ATRP. Kinetic studies demonstrated the controlled/living character of the employed polymerization methods. The thermal properties and self‐assembly in aqueous medium of the graft copolymers were dependent on their composition. The resulting polymeric materials showed low cytotoxicity toward L929 cells, demonstrating their potential for biomedical applications. This type of materials containing cationic side chains tethered to biocompatible and biodegradable segments could be the basis for promising candidates as drug and gene delivery systems.

Transient Destabilization of Biological Membranes Contributes to the Superior Performance of Star-Shaped PDMAEMA in Delivering pDNA

“…Polymer stock solutions were prepared in sterile ultrapure PCR-water (Sigma-Aldrich) as 1.25 mg mL –1 (l-PEI) and 1.82 mg mL –1 (Nanostar) and diluted for use as indicated. LD 50 values were 12.1 μg mL –1 for l-PEI 66 and 500 μg mL –1 for Nanostar 11 as determined by MTT assay.…”

“…In the previous studies, we have investigated the physicochemical properties of these PPs and established the indicated N/P ratios as optimal for transfection with these polymers. 11 , 12 , 56 , 66 The mixture was vortexed for 10 s and incubated for 20 min at room temperature. Then, one volume of a 0.002% TB solution or a 2 μg mL –1 PI solution was added.…”

Generation of Recombinant Primary Human B Lymphocytes Using Non-Viral Vectors

“…The better survival of the cells in case of transfection with l-PEI may also be ascribed to the lower polymer densities (6.0 to 39.0 µg per 10 6 cells for l-PEI, 22.0 to 144.0 µg per 10 6 cells for the nano-stars) and polymer concentrations (0.6 to 4.0 µg mL −1 for l-PEI, 2.0 to 14.0 µg mL −1 for the nano-stars) required to reach the indicated N/P ratios (see Table S1 for details). However, for both polycations, the polymer concentration at the highest N/P ratio was still below the LD 50 values recorded for free l-PEI (12 µg mL −1 ) and nano-stars (39 µg mL −1 ) in L929 cells (MTT assay) by our group [ 45 ]. Previously, we have shown that human primary T cells have a two-fold higher sensitivity to these polycations than the L929 cells and some similarity can be presumed for primary B cells [ 37 ].…”

Development of poly(ethyleneimine) grafted amphiphilic copolymers: Evaluation of their cytotoxicity and ability to complex DNA

“…This trend agreed with the results of previous reports for analogous materials. 20,23 The trend also indicate that the cellular viability is sensitive to the grafting density, as deduced from the LD 50 values obtained for the PP6Ly series.…”

“…The structure and composition of these materials were confirmed by 1 H NMR, as previously reported (Supplemental Figure S1). 23 Then, mPEG-b-P(CL-co-(MPC-g-lPEI)) copolymers were synthesized by grafting azideterminated lPEI (lPEI-N 3 ) to mPEG-b-P(CLco-MPC) backbones through the alkyne groups via click chemistry. First, lPEI-N 3 was obtained by the acidic hydrolysis of poly(2-methyl-2-oxazoline) α-benzyl ω-azide terminated (PMeOx-N3, M n ~5000) at 100°C using an excess of aqueous HCl.…”