Target-guided isolation and purification of antioxidants from Selaginella sinensis by offline coupling of DPPH-HPLC and HSCCC experiments

Zhang, Yuping; Shi, Shuyun; Wang, Yuanxi; Huang, Kelong

doi:10.1016/j.jchromb.2010.12.004

Cited by 71 publications

(41 citation statements)

References 28 publications

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“…The origins of these samples covered 32 regions, including the Guizhou, Yunnan, Sichuan, Guangxi, Jiangsu, and Anhui provinces. Samples from almost all medicinal plants of the family Selaginellaceae, such as S. tamariscina and S. puluinata , which are included in the Chinese Pharmacopoeia [22], and S. sinensis , which is endemic to China [23], were collected. All plant species were identified by Professor Peishan Wang at the Institute of Biology, Guizhou Academy.…”

Section: Methodsmentioning

confidence: 99%

Application of the ITS2 Region for Barcoding Medicinal Plants of Selaginellaceae in Pteridophyta

Song

Cao

et al. 2013

PLoS ONE

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BackgroundSelaginellaceae is a family of nonseed plants with special evolutionary significance. Plants of the family Selaginellaceae are similarly shaped and easily confused, complicating identification via traditional methods. This study explored, for the first time, the use of the DNA barcode ITS2 to identify medicinal plants of the Selaginellaceae family.Methodology/Principal FindingsIn our study, 103 samples were collected from the main distribution areas in China; these samples represented 34 species and contained almost all of the medicinal plants of Selaginellaceae. The ITS2 region of the genome was amplified from these samples and sequenced using universal primers and reaction conditions. The success rates of the PCR amplification and sequencing were 100%. There was significant divergence between the interspecific and intraspecific genetic distances of the ITS2 regions, while the presence of a barcoding gap was obvious. Using the BLAST1 and nearest distance methods, our results proved that the ITS2 regions could successfully identify the species of all Selaginellaceae samples examined. In addition, the secondary structures of ITS2 in the helical regions displayed clear differences in stem loop number, size, position, and screw angle among the medicinal plants of Selaginellaceae. Furthermore, cluster analysis using the ITS2 barcode supported the relationship between the species of Selaginellaceae established by traditional morphological methods.ConclusionThe ITS2 barcode can effectively identify medicinal plants of Selaginellaceae. The results provide a scientific basis for the precise identification of plants of the family Selaginellaceae and the reasonable development of these resources. This study may broaden the application of DNA barcoding in the medicinal plant field and benefit phylogenetic investigations.

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Section: Methodsmentioning

confidence: 99%

Application of the ITS2 Region for Barcoding Medicinal Plants of Selaginellaceae in Pteridophyta

Song

Cao

et al. 2013

PLoS ONE

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“…In the experiment, the fragmentation mechanisms of the 29 compounds were analyzed by using electrospray ionization ion trap mass spectrometry (ESI‐ITMS) and atmospheric pressure chemical ionization ion trap mass spectrometry (APCI‐ITMS) in the multi‐stage MS full scan mode. These compounds included streblusol D ( 1 ), magonlignan A 4'‐ O ‐α‐D‐glucopyranoside ( 2 ), magnolol ( 3 ), magnaldehyde D ( 4 ), erythro ‐strebluslignanol ( 5 ), threo ‐strebluslignanoxiranol ( 6 ), strebluslignanphenol ( 7 ), threo ‐strebluslignanol A ( 8 ), erythro ‐strebluslignanol B ( 9 ), erythro ‐strebluslignanol C ( 10 ), strebluslignanol D ( 11 ), strebluslignanone ( 12 ), (7' R ,8' R ,7" R ,8" R )‐ threo ‐strebluslignanol F ( 13 ), (7' R ,8' S ,7" R ,8" S )‐ erythro ‐strebluslignanol G ( 14 ), isomagnaldehyde ( 15 ), isostrebluslignaldehyde ( 16 ), isomagnolol ( 17 ), myricetin ( 18 ), naringenin ( 19 ), kaempferol‐3‐ O ‐β‐D‐glucopyranoside ( 20 ), kaempferol‐7‐ O ‐α‐L‐rhamnoside ( 21 ), kaempferol‐3,7‐ O ‐α‐L‐dirhamnoside ( 22 ), kaempferol‐3‐ O ‐β‐D‐glucopyranosyl‐7‐ O ‐α‐L‐rhamnopyranoside ( 23 ), ginkgetin ( 24 ), α‐boswellic acid ( 25 ), β‐boswellic acid ( 26 ), oleanolic acid ( 27 ), β‐amyrin ( 28 ) and β‐amyrenone ( 29 ). Their structures are shown in Fig.…”

Section: Resultsmentioning

confidence: 97%

“…In the experiment, the fragmentation mechanisms of the 29 compounds were analyzed by using electrospray ionization ion trap mass spectrometry (ESI-ITMS) and atmospheric pressure chemical ionization ion trap mass spectrometry (APCI-ITMS) in the multi-stage MS full scan mode. These compounds included streblusol D (1), [3] magonlignan A 4'-O-α-D-glucopyranoside (2), magnolol (3), magnaldehyde D (4), erythro-strebluslignanol (5), threo-strebluslignanoxiranol (6), strebluslignanphenol (7), threo-strebluslignanol A (8), erythro-strebluslignanol B (9), erythrostrebluslignanol C (10), strebluslignanol D (11), strebluslignanone (12), (7'R,8'R,7"R,8"R)-threo-strebluslignanol F (13), (7'R,8'S,7"R,8"S)erythro-strebluslignanol G (14), isomagnaldehyde (15), isostrebluslignaldehyde (16), isomagnolol (17), myricetin (18), naringenin (24), [22][23][24] α-boswellic acid (25), β-boswellic acid (26), oleanolic acid (27), β-amyrin (28) and β-amyrenone (29). Their structures are shown in wileyonlinelibrary.com/journal/rcm Reference compounds 1, 2, 4, 8-11 and 25 were separately injected and detected in the negative ion mode using the ESI source.…”

Section: Resultsmentioning

confidence: 99%

Tandem mass spectrometric fragmentation behavior of lignans, flavonoids and triterpenoids in Streblus asper

CuiPing

et al. 2014

Rapid Comm Mass Spectrometry

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“…Hinokiflavone, a biflavone which occurs in leaves of M. glyptostroboides , has been identified as a potent antioxidant using hyphenated HPLC-DPPH (Zhang et al 2011). The compound used for these studies was, however, not isolated from M. glyptostroboides .…”

Section: Antioxidant Activitymentioning

confidence: 99%

Growing with dinosaurs: natural products from the Cretaceous relict Metasequoia glyptostroboides Hu & Cheng—a molecular reservoir from the ancient world with potential in modern medicine

et al. 2015

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After the sensational rediscovery of living exemplars of the Cretaceous relict Metasequoia glyptostroboides—a tree previously known exclusively from fossils from various locations in the northern hemisphere, there has been an increasing interest in discovery of novel natural products from this unique plant source. This article includes the first complete compilation of natural products reported from M. glyptostroboides during the entire period in which the tree has been investigated (1954–2014) with main focus on the compounds specific to this plant source. Studies on the biological activity of pure compounds and extracts derived from M. glyptostroboides are reviewed for the first time. The unique potential of M. glyptostroboides as a source of bioactive constituents is founded on the fact that the tree seems to have survived unchanged since the Cretaceous era. Since then, its molecular defense system has resisted the attacks of millions of generations of pathogens. In line with this, some recent landmarks in Metasequoia paleobotany are covered. Initial spectral analysis of recently discovered intact 53 million year old wood and amber of Metasequoia strongly indicate that the tree has remained unchanged for millions of years at the molecular level.

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Target-guided isolation and purification of antioxidants from Selaginella sinensis by offline coupling of DPPH-HPLC and HSCCC experiments

Cited by 71 publications

References 28 publications

Application of the ITS2 Region for Barcoding Medicinal Plants of Selaginellaceae in Pteridophyta

Application of the ITS2 Region for Barcoding Medicinal Plants of Selaginellaceae in Pteridophyta

Tandem mass spectrometric fragmentation behavior of lignans, flavonoids and triterpenoids in Streblus asper

Growing with dinosaurs: natural products from the Cretaceous relict Metasequoia glyptostroboides Hu & Cheng—a molecular reservoir from the ancient world with potential in modern medicine

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