Target-induced multiple palindrome-mediated strand displacement amplification of Scarecrow-shaped DNA nanoprobe for ultrasensitive detection of MicroRNA

Exploration of Water-Soluble Natural AIEgens Boosting Label-Free Turn-on Fluorescent Sensing in a DNA Hydrogel

“…The limit of detection (LOD) was estimated to be 0.049 fM for miRNA-21 detection (LOD = 3σ/S, where S is the slope of the corresponding calibration curve and σ is the standard deviation of the blank signals), which is lower than those of the previously reported assay methods (Table S6). ,− As a result, a highly sensitive miRNA assay can be realized by monitoring the change in the fluorescence intensity of the system. Moreover, DNA labeled with fluorophores was avoided by the EDC method and water-soluble AIEgen, leading to a simplified procedure and low cost.…”

Robust nontarget DNA-triggered catalytic hairpin assembly amplification strategy for the improved sensing of microRNA in complex biological matrices

“…Thermocycling amplification techniques, such as polymerase chain reaction (PCR) 24 and ligase chain reaction (LCR), 25 require thermo-stable DNA polymerase or ligase and thermal machines. As an alternative, a variety of isothermal nucleic acid amplification strategies, such as rolling circle amplification (RCA), [26][27][28] strand displacement amplification (SDA), 29,30 hybridization chain reaction (HCR), 31,32 catalytic hairpin assembly (CHA), 33 helicase-dependent isothermal amplification (HDA), 34,35 and entropy-driven toehold-mediated DNA strand displacement reaction, 36 have been widely developed. These isothermal amplification techniques do not need a specific thermal cycler or expensive enzymes, and thus offer the advantages of instrument-free operation, low-cost, and time saving.…”