Target region sequencing and applications in plants

Li, Zenglu

doi:10.1007/s12892-020-00056-3

Cited by 5 publications

(5 citation statements)

References 104 publications

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“…The most important step of target RNA-seq is the design of the specific probes that can be customized to meet the specific needs of each experiment. Since only the genes of interest are sequenced, the coverage can also be very high, allowing to increase the power to assemble low expression transcripts (Ostezan et al, 2021).…”

Section: High-throughput Genotyping Techniques Exome and Rna Sequencingmentioning

confidence: 99%

Genetic approaches to exploit landraces for improvement of Triticum turgidum ssp. durum in the age of climate change

et al. 2023

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Addressing the challenges of climate change and durum wheat production is becoming an important driver for food and nutrition security in the Mediterranean area, where are located the major producing countries (Italy, Spain, France, Greece, Morocco, Algeria, Tunisia, Turkey, and Syria). One of the emergent strategies, to cope with durum wheat adaptation, is the exploration and exploitation of the existing genetic variability in landrace populations. In this context, this review aims to highlight the important role of durum wheat landraces as a useful genetic resource to improve the sustainability of Mediterranean agroecosystems, with a focus on adaptation to environmental stresses. We described the most recent molecular techniques and statistical approaches suitable for the identification of beneficial genes/alleles related to the most important traits in landraces and the development of molecular markers for marker-assisted selection. Finally, we outline the state of the art about landraces genetic diversity and signature of selection, already identified from these accessions, for adaptability to the environment.

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Section: High-throughput Genotyping Techniques Exome and Rna Sequencingmentioning

confidence: 99%

Genetic approaches to exploit landraces for improvement of Triticum turgidum ssp. durum in the age of climate change

et al. 2023

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“…Targeted DNA capture and sequencing analyses have been widely deployed in plant genomic studies (Ostezan et al ., 2021). For invitro targeted assay in plant genomes, FENGC possesses multiple advantages compared with other methods, including high liberty for customer design, high performance in sequencing depth, target number, on- target ratio, and low cost (Table 1).…”

Section: Resultsmentioning

confidence: 99%

Single-molecule long-read methylation profiling reveals regional DNA methylation regulated by Elongator Complex Subunit 2 in Arabidopsis roots experiencing spaceflight

Zhou

Gauthier

Kladde

et al. 2022

Preprint

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Flap-Enabled Next-Generation Capture (FENGC), a novel targeted DNA capture technique, has been applied to Arabidopsis root samples from the Advanced Plant Experiment-04 - Epigenetic Expression (APEX-04) experiment onboard the International Space Station (ISS). Single-molecule long-read profiling reveals regional DNA methylation alteration that was not previously uncovered by population-based methods during spaceflight.

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“…[29] ezRAD-seq [30] target-seq [31] Regardless of the methodology used, if the allelic variant(s) is responsible for a favorable trait, it can be subsequently introgressed into an elite variety through a combination of conventional breeding and molecular marker-assisted selection (MAS). MAS is defined as a process where molecular markers associated with a specific trait of interest are used for the selection of the trait itself.…”

Section: Gbsmentioning

confidence: 99%

The New Green Challenge in Urban Planning: The Right Genetics in the Right Place

et al. 2022

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The creation of green areas within urban centers was born as a response to profoundly different problems, such as the demographic increase and the progressive urbanization of landscapes. Moreover, up to date, the genetics of plants has not been considered for urban contexts. The purpose of this review is to discuss the state of the art and the advantages in planning genetic improvement for plants for urban destinations, also providing technical information, that may contribute in a concrete way. Firstly, recent genomic sources and their potential contribution to breeding programs are discussed. Then, dividing the urban scenarios into four macro areas (i.e., urban/metropolitan parks, urban gardens, road verges and roofs/terraces/balconies), we described the role of genetics in the adaptability and sustainability of plants in these different contexts. In addition, we analyzed the genetic traits plants need to provide services for a city environment and population (e.g., pollution reduction, biodiversity conservation, soil stability, and food production).

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Target region sequencing and applications in plants

Cited by 5 publications

References 104 publications

Genetic approaches to exploit landraces for improvement of Triticum turgidum ssp. durum in the age of climate change

Genetic approaches to exploit landraces for improvement of Triticum turgidum ssp. durum in the age of climate change

Single-molecule long-read methylation profiling reveals regional DNA methylation regulated by Elongator Complex Subunit 2 in Arabidopsis roots experiencing spaceflight

The New Green Challenge in Urban Planning: The Right Genetics in the Right Place

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