Target-Site Resistance Mechanisms to Tribenuron-methyl and Cross-resistance Patterns to ALS-inhibiting Herbicides of Catchweed Bedstraw (<i>Galium aparine</i>) with Different ALS Mutations

Deng, Wei; Di, Yingjie; Cai, Jingxuan; Chen, Yueyang; Yuan, Shuzhong

doi:10.1017/wsc.2018.70

Cited by 27 publications

(16 citation statements)

References 34 publications

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“…In these populations, the NTSR mechanism may be dominant, and further research is needed. The Trp-574-Leu mutation was mainly responsible for penoxsulam resistance in E. crus-galli, and the same substitution has been documented in other ALS inhibitor-resistant weeds, including catchweed bedstraw (Galium aparine L.) (Deng et al 2019b) . The variation in ALS copy number was also observed in shortawn foxtail (Alopecurus aequalis Sobol.)…”

Section: Cloning and Sequence Analysis Of Als And Accase Genesmentioning

confidence: 64%

Investigating the resistance levels and mechanisms to penoxsulam and cyhalofop-butyl in barnyardgrass (Echinochloa crus-galli) from Ningxia Province, China

Yang

Zhang

et al. 2021

Weed Sci

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Barnyardgrass (Echinochloa crus-galli) is a noxious grass weed which infests rice fields and causes huge crop yield losses. In this study, we collected twelve E. crus-galli populations from rice ﬁelds of Ningxia province in China and investigated the resistance levels to acetolactate synthase (ALS) inhibitor penoxsulam and acetyl-CoA carboxylase (ACCase) inhibitor cyhalofop-butyl. The results showed that eight populations exhibited resistance to penoxsulam and four populations evolved resistance to cyhalofop-butyl. Moreover, all of the four cyhalofop-butyl-resistant populations (NX3, NX4, NX6 and NX7) displayed multiple-herbicide-resistance (MHR) to both penoxsulam and cyhalofop-butyl. The alternative herbicides bispyribac-sodium, metamifop and fenoxaprop-P-ethyl cannot effectively control the MHR plants. To characterize the molecular mechanisms of resistance, we ampliﬁed and sequenced the target-site encoding genes in resistant and susceptible populations. Partial sequences of three ALS genes and six ACCase genes were examined. A Trp-574-Leu mutation was detected in EcALS1 and EcALS3 in two high-level (65.84- and 59.30-fold) penoxsulam-resistant populations NX2 and NX10, respectively. In addition, one copy (EcACC4) of ACCase genes encodes a truncated aberrant protein due to a frameshift mutation in E. crus-galli populations. None of amino acid substitutions that are known to confer herbicide resistance were detected in ALS and ACCase genes of MHR populations. Our study reveals the widespread of multiple-herbicide resistant E. crus-galli populations at Ningxia province of China that exhibit resistance to several ALS and ACCase inhibitors. Non-target-site based mechanisms are likely to be involved in E. crus-galli resistance to the herbicides, at least in four MHR populations.

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Section: Cloning and Sequence Analysis Of Als And Accase Genesmentioning

confidence: 64%

Investigating the resistance levels and mechanisms to penoxsulam and cyhalofop-butyl in barnyardgrass (Echinochloa crus-galli) from Ningxia Province, China

Yang

Zhang

et al. 2021

Weed Sci

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“…This study found two amino acid substitutions (Trp‐574‐Leu or Gly‐654‐Tyr) in ALS in the imazethapyr resistant A. retroflexus population. The Trp‐574‐Leu substitution has previously been documented as providing ALS‐inhibiting herbicide resistance in many resistant weed species including cocklebur ( Xanthium strumarium ), 22 A. retroflexus , 18 flixweed ( Descurainia sophia L.), 23 and bedstraw ( Galium aparine ) 24 . However, there has not been any report on the Gly‐654‐Tyr substitution endowing ALS resistance in plant species.…”

Section: Discussionmentioning

confidence: 99%

Amino acid substitution (Gly‐654‐Tyr) in acetolactate synthase (ALS) confers broad spectrum resistance to ALS‐inhibiting herbicides

Cao

Zhou

Huang

2021

Pest Management Science

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BACKGROUND Amaranthus retroflexus L. is a problematic weed in agricultural fields. In China, the repeated use of acetolactate synthase (ALS) inhibiting herbicides has led to the evolution of many A. retroflexus resistant populations. The objective of this research was to investigate the physiological and molecular basis for resistance to ALS‐inhibiting herbicides in A. retroflexus. RESULTS Sequence analysis of the ALS revealed a Trp to Leu substitution at amino acid position 574 (Trp‐574‐Leu), and a previously unreported substitution of Gly to Tyr substitution at 654 (Gly‐654‐Tyr) in the resistant A. retroflexus population. A purified R‐Tyr654 subpopulation homozygous for the Gly‐654‐Tyr mutation was generated and characterized in response to five different classes of ALS‐inhibiting herbicides. Dose–response analysis revealed that the R‐Tyr654 population was highly resistant to two imidazolinones (imazethapyr, >42‐fold; imazamox, 48.3‐fold), one triazolopyrimidine (flumetsulam, 57.4‐fold), and one sulfonylamino‐carbonyltriazolinone (flucarbazone, 17.3‐fold). Moreover, it was moderately resistant to two sulfonylureas (thifensulfuron‐methyl, 4.9‐fold; nicosulfuron, 9.1‐fold), and one pyrimidinylthio‐benzoate (bispyribac‐sodium, 3.1‐fold). An in vitro ALS activity assay showed that ALS with the Gly‐654‐Tyr substitution was resistant to all the tested ALS‐inhibiting herbicides. However, the field rate of herbicides binding other sites of action demonstrated effective control of the R‐Tyr654 population. CONCLUSION These results indicate that the molecular basis of ALS‐inhibiting herbicide resistance in A. retroflexus was caused by the Trp‐574‐Leu and Gly‐654‐Tyr substitutions in the ALS. This is the first report of Gly‐654‐Tyr substitution in ALS, and this substitution confers broad spectrum resistance to ALS‐inhibiting herbicides. © 2021 Society of Chemical Industry.

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“…For example, the Pro-197-Ser mutation in D. sophia exhibited cross-resistance to SU and TP herbicides and showed no cross-resistance to PTB and IMI herbicides (Deng et al 2014). The same mutation displayed resistance to SU, TP, and PTB herbicides in M. aquaticum (Liu et al 2013) and broad-spectrum resistance to SU, TP, PTB, and IMI herbicides in E. prostrata (Li et al 2017) and G. aparine (Deng et al 2019). Therefore, it is necessary to determine the specific cross-resistance patterns for the newly identified herbicide-resistant biotype.…”

Section: Cross-resistance To Other Als-inhibiting Herbicidesmentioning

confidence: 99%

Molecular basis of resistance to bensulfuron-methyl and cross-resistance patterns to ALS-inhibiting herbicides in Ludwigia prostrata

et al. 2021

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Ludwigia prostrata Roxb. is a problematic weed in rice fields in China, and acetolactate synthase (ALS)-inhibiting herbicides (e.g., bensulfuron-methyl) are widely used for the management of broadleaf weeds. Recently, a L. prostrata biotype (JS-R) that failed to be controlled with ALS-inhibiting herbicides was found in Jiangsu Province, China. This study aims to determine the level and molecular mechanism of resistance to bensulfuron-methyl in this JS-R biotype, and evaluate the cross-resistance spectrums to other ALS-inhibiting herbicides. The dose-response assays indicated that the JS-R L. prostrata biotype had evolved 21.2-fold resistance to bensulfuron-methyl compared to the susceptible biotype (JS-S). ALS gene sequencing revealed that a nucleotide mutation (CCA to TCA) at codon 197, resulting in a Pro-197-Ser mutation, was detected in the resistant plants. Moreover, the JS-R biotype contained the Pro-197-Ser resistance mutation showed cross-resistance to pyrazosulfuron-ethyl (12.0-fold), but was sensitive to penoxsulam, bispyribac-sodium and imazethapyr, which may serve as alternative herbicides to control the resistant L. prostrata biotype. This is the first confirmation of a L. prostrata biotype resistant to bensulfuron-methyl due to a Pro-197-Ser resistance mutation in the ALS gene.

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Target-Site Resistance Mechanisms to Tribenuron-methyl and Cross-resistance Patterns to ALS-inhibiting Herbicides of Catchweed Bedstraw (Galium aparine) with Different ALS Mutations

Cited by 27 publications

References 34 publications

Investigating the resistance levels and mechanisms to penoxsulam and cyhalofop-butyl in barnyardgrass (Echinochloa crus-galli) from Ningxia Province, China

Investigating the resistance levels and mechanisms to penoxsulam and cyhalofop-butyl in barnyardgrass (Echinochloa crus-galli) from Ningxia Province, China

Amino acid substitution (Gly‐654‐Tyr) in acetolactate synthase (ALS) confers broad spectrum resistance to ALS‐inhibiting herbicides

Molecular basis of resistance to bensulfuron-methyl and cross-resistance patterns to ALS-inhibiting herbicides in Ludwigia prostrata

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