Targeted and untargeted mass spectrometric approaches in discrimination between Myrtus communis cultivars from Sardinia region

Abstract

In the present study, the discrimination of phytochemical content of Myrtus communis berries from different geographical origin and cultivars was explored by Liquid Chromatography-Electrospray Ionization-Fourier Transform-Mass Spectrometry (LC-ESI-FT-MS) metabolic profiling and quantitative analysis. Experiments were carried on myrtle plants grown in an experimental area of Sardinia region, obtained by the germination of seeds taken from berries collected in each part of the region. A preliminary untargeted approach on fruit's extracts was realized by collecting LC-ESI-FT-(Orbitrap)-MS data obtained by operating in negative ion mode and performing principal component analysis with the result of differentiation of samples. In a second step, targeted analysis with a reduced number of variables was realized. A data matrix was obtained by the data fusion of positive and negative ionization LC-ESI-MS results, by using as variables the peak areas of each known compounds. By the observation of principal component analysis, results found that anthocyanins, and mainly derivatives of cyanidin, are the principal marker compounds responsive for the discrimination of samples based on the geographical origin of the seeds. Based on this finding, finally, an LC-diode array detector method was developed, validated and applied for the quantitative analysis of berries' extracts based on 11 commercial standard compounds corresponding to the identified markers. Copyright © 2016 John Wiley & Sons, Ltd.

Fibroblast Proliferation and Migration in Wound Healing by Phytochemicals: Evidence for a Novel Synergic Outcome

“…The HPLC experiments were performed according to a method previously reported by Sarais et al and appropriately modified [33]. Briefly, the analysis was performed on an Agilent 1100 series HPLC system (Agilent Technologies, Milan, Italy) equipped with a quaternary pump, a vacuum degasser, an autosampler and a thermostated column compartment and coupled with a diode array detector (DAD) UV6000LP (Thermo Finnigan, Milan, Italy) using a data acquisition software ChromQuest version 4.0.…”

Alternative Ultrasound-Assisted Method for the Extraction of the Bioactive Compounds Present in Myrtle (Myrtus communis L.)

“…For the reason, UAE has been used for the extraction of the antioxidant compounds found in fruits matrices, such as papayas [24], mulberries [25], oranges [26] or sugarcane [27]. With regard to myrtle, some articles have been found in literature where UAE is used to extract biological compounds from myrtle berries [28,29], but it has not yet been carried out a thorough optimization and development of this technique for the specific extraction of phenolic compounds and anthocyanins. This would be very convenience, since the correct applicability would improve the analysis of the raw material, which would enhance the quality, for example, of liquor, as the main product obtained from myrtle.…”

Development of New Analytical Microwave-Assisted Extraction Methods for Bioactive Compounds from Myrtle (Myrtus communis L.)

“…The quantities of phenolic compounds and anthocyanins present in myrtle berries are extraordinarily high [ 11 , 12 ]. The major phenolic compounds identified in myrtle are quercetin 3- O -galactoside, quercetin 3- O -rhamnoside, myricetin 3- O -rhamnoside, quercetin 3- O -glucoside, ellagic acid, and myricetin [ 13 , 14 ]. The major anthocyanins identified are delphinidin 3,5- O -diglucoside, delphinidin 3- O -glucoside, cyanidin 3- O -galactoside, cyanidin 3- O -glucoside, cyanidin 3- O -arabinoside, petunidin 3- O -glucoside, delphinidin 3- O -arabinoside, peonidin 3- O -glucoside, malvidin 3- O -glucoside, petunidin 3- O -arabinoside, and malvidin 3- O -arabinoside [ 15 , 16 ].…”