Targeted antigen delivery to dendritic cell via functionalized alginate nanoparticles for cancer immunotherapy

Zhang, Chuangnian; Shi, Gaona; Zhang, Ju; Song, Huijuan; Niu, Jinfeng; Shi, Shengbin; Huang, Pingsheng; Wang, Yanming; Wang, Weiwei; Li, Chen; Kong, Deling

doi:10.1016/j.jconrel.2017.04.020

Cited by 146 publications

(98 citation statements)

References 41 publications

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“…In fact, a Gantrez ®® AN-based device is expected to act as an active Th1 adjuvant through TLR exploitation [20]. In addition, the presence of mannosamine could facilitate the targeting properties of these nanocarriers for dendritic cells [21]. Here, we report for the first time the immunoadjuvant capacity of this nanoparticle formulation for oral vaccination in BALB/c mice and sows.…”

Section: Introductionmentioning

confidence: 90%

Oral Immunogenicity in Mice and Sows of Enterotoxigenic Escherichia Coli Outer-Membrane Vesicles Incorporated into Zein-Based Nanoparticles

Matías

Brotons

Cenoz³

et al. 2019

Vaccines

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Enterotoxigenic Escherichia coli (ETEC) strains are a major cause of illness and death in neonatal and recently weaned pigs. The immune protection of the piglets derives from maternal colostrum, since this species does not receive maternal antibodies through the placenta. In the present study, outer membrane vesicles (OMVs) obtained from main ETEC strains involved in piglet infection (F4 and F18 serotypes), encapsulated into zein nanoparticles coated with Gantrez®® AN-mannosamine conjugate, were used to orally immunize mice and pregnant sows. Loaded nanoparticles were homogeneous and spherical in a shape, with a size of 220–280 nm. The diffusion of nanoparticles through porcine intestinal mucus barrier was assessed by a Multiple Particle Tracking technique, showing that these particles were able to diffuse efficiently (1.3% diffusion coefficient), validating their oral use. BALB/c mice were either orally immunized with free OMVs or encapsulated into nanoparticles (100 µg OMVs/mouse). Results indicated that a single dose of loaded nanoparticles was able to elicit higher levels of serum specific IgG1, IgG2a and IgA, as well as intestinal IgA, with respect to the free antigens. In addition, nanoparticles induced an increase in levels of IL-2, IL-4 and IFN-γ with respect to the administration of free OMVs. Orally immunized pregnant sows with the same formulation elicited colostrum-, serum- (IgG, IgA or IgM) and fecal- (IgA) specific antibodies and, what is most relevant, offspring suckling piglets presented specific IgG in serum. Further studies are needed to determine the infection protective capacity of this new oral subunit vaccine

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Section: Introductionmentioning

confidence: 90%

Oral Immunogenicity in Mice and Sows of Enterotoxigenic Escherichia Coli Outer-Membrane Vesicles Incorporated into Zein-Based Nanoparticles

Matías

Brotons

Cenoz³

et al. 2019

Vaccines

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“…Efficient antigen delivery to antigen processing cells is a prerequisite for the induction of immune response . DCs uptake of FITC‐OVA and FITC‐OVA‐Cu‐HVs was quantitatively determined using flow cytometry.…”

Section: Resultsmentioning

confidence: 99%

Antigen‐Inorganic Hybrid Flowers‐Based Vaccines with Enhanced Room Temperature Stability and Effective Anticancer Immunity

Liu

Zhu

Lü

et al. 2019

Adv Healthcare Materials

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Particle‐based antigen carriers as adjuvants play an important role in vaccine development. Herein, an antigen‐inorganic hybrid flower‐like particle is developed as a novel vaccine carrier. Model antigen ovalbumin (OVA)‐copper (II) sulfate hybrid vaccines (OVA‐Cu‐HVs) are mildly and facilely constructed through a biomimetic mineralization process. OVA‐Cu‐HVs facilitate cellular uptake in antigen‐presenting cells and the internalization of OVA‐Cu‐HVs involves macropinocytosis‐mediated endocytosis. OVA‐Cu‐HVs can release OVA in a pH‐responsive behavior and promote cytosolic release of antigen to enhance antigen cross‐presentation. Immunization with OVA‐Cu‐HVs promotes the maturation of dendritic cells in draining lymph nodes, induces robust antigen‐specific T lymphocyte response, and inhibits tumor growth in vivo. In addition, OVA‐Cu‐HVs are efficacious after being stored for 4 weeks at room temperature and are expected to simplify vaccine storage and lower the cost of cold storage for transportation. Looking forward, OVA‐Cu‐HVs may hold strong potential to be as an effective vaccine delivery platform, which will facilitate the application of organic–inorganic hybrid flowers in biomedical areas.

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“…Design of pH‐responsive nanocarriers that are internalized by endocytosis, promote the endosomal escape (pH 4–6), and release the exogenous antigens into the cytosol: in this cell compartment they are cleaved into peptides, imported into the endoplasmic reticulum, and loaded onto MHC class I, which presents antigen to CD8 + T cells, a process known as cross presentation. Reproduced with permission . Copyright 2017, Elsevier B.V.…”

Section: Cancer Immunotherapymentioning

confidence: 99%

“…For this purpose, pH‐responsive moieties can be introduced to engineer NPs with different degrees of acid‐induced degradability that will affect the magnitude and pathway of the antigen‐presentation process . Furthermore, pH‐sensitive Schiff base bonds can be created in order to increase the cytosolic release of the antigen and enhance the cross‐presentation process . PEI is a cationic polymer that has been widely studied due to its capacity to be efficiently uptaken by cells and induce the cross‐presentation of the antigen on MHC class I molecules via both endosome escape and a lysosomal processing mechanism .…”

Section: Cancer Immunotherapymentioning

confidence: 99%

Immunostimulation and Immunosuppression: Nanotechnology on the Brink

et al. 2018

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Immunotherapy is revolutionizing current therapies in cancer and in autoimmune diseases, showing impressive results in clinical trials. Recently, the development of innovative nano‐biomaterials has been focusing on the interactions between such materials and the immune system, both in the adaptive and innate branches. Biomaterials can display immunostimulative, neutral, or immunosuppressive interactions. Nanoparticles displaying immunostimulative potential are employed in the formulation of cancer vaccines, while immunosuppressive materials are being researched for the development of vaccines able to induce tolerance in autoimmune diseases. Here, the properties influencing the immunomodulation effect of nanomaterials are analyzed, followed by a detailed review of the recent applications of nanomaterials for the delivery of antigens, adjuvants, chemotherapeutics, and immunomodulatory drugs.

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Targeted antigen delivery to dendritic cell via functionalized alginate nanoparticles for cancer immunotherapy

Cited by 146 publications

References 41 publications

Oral Immunogenicity in Mice and Sows of Enterotoxigenic Escherichia Coli Outer-Membrane Vesicles Incorporated into Zein-Based Nanoparticles

Oral Immunogenicity in Mice and Sows of Enterotoxigenic Escherichia Coli Outer-Membrane Vesicles Incorporated into Zein-Based Nanoparticles

Antigen‐Inorganic Hybrid Flowers‐Based Vaccines with Enhanced Room Temperature Stability and Effective Anticancer Immunity

Immunostimulation and Immunosuppression: Nanotechnology on the Brink

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