Targeted antioxidant delivery modulates mitochondrial functions, ameliorates oxidative stress and preserve sperm quality during cryopreservation

Tiwari, Swati; Dewry, Raju Kumar; Srivastava, Rashika; Nath, Sapna; Mohanty, T. K.

doi:10.1016/j.theriogenology.2021.11.013

Cited by 39 publications

(27 citation statements)

References 135 publications

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“…An intact sperm plasma membrane is a prerequisite for acrosome reaction initiation and oocyte fusion. In the present study, the higher plasma membrane integrity in MT200+Tr could be explained by MitoQ effects on ROS reduction to prevent membrane lipid peroxidation [37]. Another possibility is that trehalose modulated membrane fluidity by interaction with the bilayer surface of the membrane [1].…”

Section: Discussionmentioning

confidence: 90%

“…This is congruent with previous studies, which have demonstrated the extender containing the concentration of 200 nM in human [21] and buffalo [38], and 150 nM in ram [40] have better sperm motility after cryopreservation. MitoQ supports ATP synthesis to maintain sperm motility through the electrons exchange of the redox cycle in the mitochondrial electron transport chain [37]. Furthermore, sperm proteins are susceptible to high ROS levels, which could negatively affect sperm flagellar movement.…”

Section: Discussionmentioning

confidence: 99%

“…ROS plays vital roles in chromatin remodeling, hyperactivation, acrosome reaction, and oocyte fusion of sperm. Following sperm cryopreservation, the superoxide anion as the principal mitochondrial ROS increases, which promotes lipid peroxidation and ultimately impairs sperm function [37]. MitoQ has been able to diminish superoxide anions in frozen-thawed sperm, which could be explained by the enhanced mitochondrial antioxidant enzymes, especially SOD [6].…”

Section: Discussionmentioning

confidence: 99%

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Protective effects of different doses of MitoQ separately and combined with trehalose on sperm function and antioxidative status of cryopreserved Markhoz goat semen

Rezaei

Bahmani²,

Mafakheri³

et al. 2022

Preprint

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The mitochondria-targeted antioxidant MitoQ has been regarded as an effective antioxidant agent against cryo-induced oxidative cellular damage. This study aimed to evaluate the use of different doses of MitoQ combined with trehalose to minimize mitochondrial impairment and oxidative stress during sperm cryopreservation of Markhoz goat. For this, semen collection was performed every 5 days from 5 bucks (10 ejaculates per buck). The ejaculates were pooled and then diluted in eight different Tris-based extenders as follows: no additives (control), 20, 200, 2000 nM of MitoQ (MT20, MT200, MT 2000, respectively), 150 mM of trehalose (Tr), MT20+Tr, MT200+Tr, MT2000+Tr. Each semen sample was frozen using a standard protocol, and sperm function and antioxidative status were evaluated after thawing. Results indicated higher total and progressive motility, acrosome and membrane integrity, superoxide dismutase, glutathione peroxidase, total antioxidant capacity, and lower DNA fragmentation and malondialdehyde in MT200+Tr than for all other groups except MT200; meanwhile, MT200 was also greater in these parameters than in the control group (P < 0.05). Furthermore, MT200 and MT200+Tr showed higher (P < 0.05) percentages of live cryopreserved sperm with high membrane mitochondrial potential than other groups. However, abnormality percentage and catalase activity of frozen-thawed sperm were not affected by treatments (P > 0.05). To conclude, we have found that supplementation of 200 nM MitoQ alone or in combination with 150 mM trehalose to semen extender improves the quality of cryopreserved sperm in goats, which is associated with enhanced antioxidant enzymatic defense and mitochondrial activity and reduced DNA fragmentation.

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Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Protective effects of different doses of MitoQ separately and combined with trehalose on sperm function and antioxidative status of cryopreserved Markhoz goat semen

Rezaei

Bahmani²,

Mafakheri³

et al. 2022

Preprint

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“…However, in both methods of preservation, the required passage of tissue from −150 °C or −80 °C to room temperature before its clinical use leads to oxidative stress within cells [ 13 ]. To take advantage of HAM properties maintained after cryopreservation or freezing, reducing the undesirable effect of increased ROS production after tissue thawing, we decided to perform a treatment with quercetin on our HAM samples before their storage, similarly to other studies in which antioxidants were used to improve well-being after tissue thawing [ 14 , 15 , 34 ]. The selection of quercetin as an antioxidant for our experiments was carried out based on evidence of its effectiveness at low concentration (50 µM) for the cryopreservation of other biological samples, such as human spermatozoa [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

“…However, the freezing-thawing of the tissue, required in both preservation methods, can induce cellular stress due to reactive oxygen species (ROS) production, leading, in turn, to cell damage such as lipid peroxidation and rupture of the mitochondrial, as well as the plasma membrane [ 13 ]. Thus, strategies able to improve the cellular well-being of cryopreserved or frozen biological samples were previously developed for their final clinical effectiveness [ 14 , 15 ]. For the same purpose, we decided to develop and compare two preservation protocols aimed at preventing ROS production within HAM tissue.…”

Section: Introductionmentioning

confidence: 99%

The Use of Quercetin to Improve the Antioxidant and Regenerative Properties of Frozen or Cryopreserved Human Amniotic Membrane

et al. 2022

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The biological properties of the human amniotic membrane (HAM) and its characteristic ability to be a reservoir of growth factors promoting wound healing make it an ideal biological dressing for the treatment of different clinical conditions, such as burns and non-healing wounds. However, the application of a preservation method on the HAM is required during banking to maintain biological tissue properties and to ensure the release overtime of protein content for its final clinical effectiveness after application on the wound bed. Although cryopreservation and freezing are methods widely used to maintain tissue properties, reactive oxygen species (ROS) are produced within tissue cellular components during their switching from frozen to thawed state. Consequently, these methods can lead to oxidative stress-induced cell injury, affecting tissue regenerative properties and its final clinical effectiveness. Taking advantage of the antioxidant activity of the natural compound quercetin, we used it to improve the antioxidant and regenerative properties of frozen or cryopreserved HAM tissues. In particular, we evaluated the oxidative damage (lipid peroxidation, malondialdehyde) as well as the regenerative/biological properties (bFGF growth factor release, wound healing closure, structure, and viability) of HAM tissue after its application. We identified the effectiveness of quercetin on both preservation methods to reduce oxidative damage, as well as its ability to enhance regenerative properties, while maintaining the unaltered structure and viability of HAM tissue. The use of quercetin described in this study appears able to counteract the side effects of cryopreservation and freezing methods related to oxidative stress, enhancing the regenerative properties of HAM. However, further investigations will need to be performed, starting from these promising results, to identify its beneficial effect when applied on burns or non-healing wounds.

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Cryopreservation of Immune Cells: Recent Progress and Challenges Ahead

Qi,

Jia,

Zhou

et al. 2024

Advanced Biology

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Cryopreservation of immune cells is considered as a key enabling technology for adoptive cellular immunotherapy. However, current immune cell cryopreservation technologies face the challenges with poor biocompatibility of cryoprotection materials, low efficiency, and impaired post‐thaw function, limiting their clinical translation. This review briefly introduces the adoptive cellular immunotherapy and the approved immune cell‐based products, which involve T cells, natural killer cells and etc. The cryodamage mechanisms to these immune cells during cryopreservation process are described, including ice formation related mechanical and osmotic injuries, cryoprotectant induced toxic injuries, and other biochemical injuries. Meanwhile, the recent advances in the cryopreservation medium and freeze‐thaw protocol for several representative immune cell type are summarized. Furthermore, the remaining challenges regarding on the cryoprotection materials, freeze‐thaw protocol, and post‐thaw functionality evaluation of current cryopreservation technologies are discussed. Finally, the future perspectives are proposed toward advancing highly efficient cryopreservation of immune cells.

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Targeted antioxidant delivery modulates mitochondrial functions, ameliorates oxidative stress and preserve sperm quality during cryopreservation

Cited by 39 publications

References 135 publications

Protective effects of different doses of MitoQ separately and combined with trehalose on sperm function and antioxidative status of cryopreserved Markhoz goat semen

Protective effects of different doses of MitoQ separately and combined with trehalose on sperm function and antioxidative status of cryopreserved Markhoz goat semen

The Use of Quercetin to Improve the Antioxidant and Regenerative Properties of Frozen or Cryopreserved Human Amniotic Membrane

Cryopreservation of Immune Cells: Recent Progress and Challenges Ahead

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