Targeted Antitubercular Peptide Nanocarriers Prepared by Flash NanoPrecipitation with Hydrophobic Ion Pairing

Ristroph, Kurt D.; McManus, Simon A.; Shetye, Gauri; Cho, Sang Hyun; Lee, Dennis; Székely, Zoltán; Sinko, Patrick J.; Franzblau, Scott G.; Prud'homme, Robert Krafft

doi:10.1002/admt.202101748

Cited by 5 publications

(2 citation statements)

References 34 publications

(51 reference statements)

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“…Parallelly, Ristroph et al [126] employed an encapsulation technique to administer the AntiTB peptide ecumycin via monodisperse nanocarriers measuring 60 nm. This was achieved through a blend of techniques: FlashPrecipitation and hydrophobic ion pairing, leading to an encapsulation efficiency of 70% and a mass loading of 24%.…”

Section: Pretomanidmentioning

confidence: 99%

Advances in Diagnostics and Drug Discovery against Resistant and Latent Tuberculosis Infection

Shleider Carnero Canales,

Marquez Cazorla,

Furtado Torres

et al. 2023

Pharmaceutics

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Latent tuberculosis infection (LTBI) represents a subclinical, asymptomatic mycobacterial state affecting approximately 25% of the global population. The substantial prevalence of LTBI, combined with the risk of progressing to active tuberculosis, underscores its central role in the increasing incidence of tuberculosis (TB). Accurate identification and timely treatment are vital to contain and reduce the spread of the disease, forming a critical component of the global strategy known as “End TB.” This review aims to examine and highlight the most recent scientific evidence related to new diagnostic approaches and emerging therapeutic treatments for LTBI. While prevalent diagnostic methods include the tuberculin skin test (TST) and interferon gamma release assay (IGRA), WHO’s approval of two specific IGRAs for Mycobacterium tuberculosis (MTB) marked a significant advancement. However, the need for a specific test with global application viability has propelled research into diagnostic tests based on molecular diagnostics, pulmonary immunity, epigenetics, metabolomics, and a current focus on next-generation MTB antigen-based skin test (TBST). It is within these emerging methods that the potential for accurate distinction between LTBI and active TB has been demonstrated. Therapeutically, in addition to traditional first-line therapies, anti-LTBI drugs, anti-resistant TB drugs, and innovative candidates in preclinical and clinical stages are being explored. Although the advancements are promising, it is crucial to recognize that further research and clinical evidence are needed to solidify the effectiveness and safety of these new approaches, in addition to ensuring access to new drugs and diagnostic methods across all health centers. The fight against TB is evolving with the development of more precise diagnostic tools that differentiate the various stages of the infection and with more effective and targeted treatments. Once consolidated, current advancements have the potential to transform the prevention and treatment landscape of TB, reinforcing the global mission to eradicate this disease.

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Section: Pretomanidmentioning

confidence: 99%

Advances in Diagnostics and Drug Discovery against Resistant and Latent Tuberculosis Infection

Shleider Carnero Canales,

Marquez Cazorla,

Furtado Torres

et al. 2023

Pharmaceutics

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“…Two recent advancements in FNP make the technology an attractive option for nanoformulating agrochemicals. The first enables the encapsulation of ionizable hydrophilic molecules, − enabling encapsulation of both hydrophobic and hydrophilic AIs, and the second replaces high-cost block copolymer stabilizers with low-cost alternatives such as lecithin, gelatin, a combination of the corn protein zein with the milk protein byproduct casein, and hydroxypropyl methylcellulose acetate succinate (HPMCAS), ,− a semisynthetic cellulose derivative used in the pharmaceutical industry. The result is a platform technology for manufacturing, at large scales and low per-unit costs, NCs capable of encapsulating a wide range of payloads.…”

Section: Introductionmentioning

confidence: 99%

Flash NanoPrecipitation as an Agrochemical Nanocarrier Formulation Platform: Phloem Uptake and Translocation after Foliar Administration

Ristroph,

Zhang,

Nava

et al. 2023

ACS Agric. Sci. Technol.

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The increasing severity of pathogenic and environmental stressors that negatively affect plant health has led to interest in developing next-generation agrochemical delivery systems capable of precisely transporting active agents to specific sites within plants. In this work, we adapt Flash NanoPrecipitation (FNP), a scalable nanocarrier (NC) formulation technology used in the pharmaceutical industry, to prepare organic core−shell NCs and study their efficacy as foliar or root delivery vehicles. NCs ranging in diameter from 55 to 200 nm, with surface zeta potentials from −40 to +40 mV, and with seven different shell material properties were prepared and studied. Shell materials included synthetic polymers poly(acrylic acid), poly(ethylene glycol), and poly(2-(dimethylamino)ethyl methacrylate), naturally occurring compounds fish gelatin and soybean lecithin, and semisynthetic hydroxypropyl methylcellulose acetate succinate (HPMCAS). NC cores contained a gadolinium tracer for tracking by mass spectrometry, a fluorescent dye for tracking by confocal microscopy, and model hydrophobic compounds (alpha tocopherol acetate and polystyrene) that could be replaced by agrochemical payloads in subsequent applications. After foliar application onto tomato plants with Silwet L-77 surfactant, internalization efficiencies of up to 85% and NC translocation efficiencies of up to 32% were observed. Significant NC trafficking to the stem and roots suggests a high degree of phloem loading for some of these formulations. Results were corroborated by confocal microscopy and synchrotron X-ray fluorescence mapping. NCs stabilized by cellulosic HPMCAS exhibited the highest degree of translocation, followed by formulations with a significant surface charge. The results from this work indicate that biocompatible materials like HPMCAS are promising agrochemical delivery vehicles in an industrially viable pharmaceutical nanoformulation process (FNP) and shed light on the optimal properties of organic NCs for efficient foliar uptake, translocation, and delivery.

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Design of Modular, 3D‐Printed Millifluidic Mixers to Enable Sequential NanoPrecipitation (SNaP) for the Tunable Synthesis of Drug‐Loaded Nanoparticles and Microparticles

Belinky,

Amri,

Lewis

et al. 2024

Adv Materials Technologies

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Sequential NanoPrecipitation (SNaP) is a nascent controlled precipitation process for the tunable formation of polymeric particles for drug delivery and bioimaging. While SNaP utilizes the same self‐assembly principles as one‐step Flash NanoPrecipitation, SNaP is a two‐step assembly process in which the particle core formation is initiated during a first mixing step followed by particle stabilization in a second mixing step. Current SNaP experimental set‐ups use commercial millifluidic mixers connected in series, which have several limitations, including the inability to access short inter‐mixer delay times (Td). A robust, 3D‐printed, modular mixer design that enables access to short Td's (〈 25 ms) not previously accessible is reported. For the first time, it is demonstrated that decoupling the assembly steps improves control over particle size, expanding the attainable size range to include both nanoparticles and microparticles. It is empirically proven that inter‐mixer Td is a key parameter for particle size control and that particle size scales with Td in agreement with Smoluchowski's model of diffusion‐limited growth. The formation of particles ranging in size from 160 nm to 1.2 µm is shown. Finally, the applicability of the new mixers is established by encapsulating fluorophores and therapeutics into particles for the first time via SNaP.

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Targeted Antitubercular Peptide Nanocarriers Prepared by Flash NanoPrecipitation with Hydrophobic Ion Pairing

Cited by 5 publications

References 34 publications

Advances in Diagnostics and Drug Discovery against Resistant and Latent Tuberculosis Infection

Advances in Diagnostics and Drug Discovery against Resistant and Latent Tuberculosis Infection

Flash NanoPrecipitation as an Agrochemical Nanocarrier Formulation Platform: Phloem Uptake and Translocation after Foliar Administration

Design of Modular, 3D‐Printed Millifluidic Mixers to Enable Sequential NanoPrecipitation (SNaP) for the Tunable Synthesis of Drug‐Loaded Nanoparticles and Microparticles

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