Targeted Charge‐Reversal Nanoparticles for Nuclear Drug Delivery

Xu, Pao; Kirk, Edward A. Van; Zhan, Yihong; Murdoch, William J.; Radosz, Maciej; Shen, Youqing

doi:10.1002/anie.200605254

Cited by 359 publications

(302 citation statements)

References 31 publications

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“…Free DOX enters cancer cells and preferentially labels their nuclei in 3 h as we have previously reported ( Figure 4 ). [ 11 ] We also noticed that in the confocal signature of the nanogel disappeared with the size increase. We think the loss of the multicompartment pattern was due to the break of disulfi de bonds and the subsequent reorganization of the nanogel.…”

Section: Communicationmentioning

confidence: 86%

Multicompartment Intracellular Self‐Expanding Nanogel for Targeted Delivery of Drug Cocktail

Bahadur

2012

Advanced Materials

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A nano cocktail, NCPD, fabricated from a pH and redox dual responsive polymer shows a multicompartment structure. The NCPD nanogel is stable in physiological environments while intracellular spontaneous swelling and fast releasing its payload. NCPD displays much stronger synergism than its free drug counterpart, which suggests that NCPD could greatly attenuate the side effects of drug cocktails while boosting synergistic anticancer effects.

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Section: Communicationmentioning

confidence: 86%

Multicompartment Intracellular Self‐Expanding Nanogel for Targeted Delivery of Drug Cocktail

Bahadur

2012

Advanced Materials

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“…A similar pH influence on zeta potential has been reported previously in the literature. [25][26][27] As a result, the use of LPH nanoparticles may dramatically enhance the concentration of the drug in cells, thereby improving drug cytotoxicity and more effectively overcoming MDR in cancer cells (Figure 4). In all cell lines studied, DTX exhibited a time-and dose-dependent cytotoxic effect.…”

Section: In Vitro Cell Studiesmentioning

confidence: 99%

Tumor-targeting, pH-sensitive nanoparticles for docetaxel delivery to drug-resistant cancer cells

Kim¹,

Tran²,

Ramasamy³

et al. 2015

IJN

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The attachment of polyethylene glycol (PEG) increases the circulation time of drug-containing nanoparticles; however, this also negatively affects cellular uptake. To overcome this problem, unique lipid polymer hybrid (LPH) nanoparticles were developed with a pH-responsive PEG layer that detached prior to cell uptake. Docetaxel (DTX) was incorporated into the lipid core of the nanoparticles, which was then shielded with the pH-responsive block co-polymer polyethylene glycol- b -polyaspartic acid (PEG- b -PAsp) using a modified emulsion method. The optimized LPH nanoparticles were ~200 nm and had a narrow size distribution. Drug release from DTX-loaded LPH (DTX-LPH) nanoparticles was pH-sensitive, which is beneficial for tumor targeting. More importantly, DTX-LPH nanoparticles were able to effectively induce apoptosis in cancer cells. The negative surface charge and PEG shell of vehicle remarkably enhanced the blood circulation and physiological activity of DTX-LPH nanoparticles compared with that of free DTX. The nanoparticles were also found to reduce the size of tumors in tumor-bearing xenograft mice. The in vivo anticancer effect of DTX-LPH nanoparticles was further confirmed by the elevated levels of caspase-3 and poly ADP ribose polymerase found in the tumors after treatment. Thus, the results suggest that this novel LPH system could be an effective new treatment for cancer.

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“…Vast majority of the laser Ag NPs is located outside the A594 cell membrane ( Figure 8A). Previous finding by Xu et al 42 demonstrated that NPs located in the nuclei or ribosomes of the cells were more toxic than those in the cytoplasm, even at low concentrations. In our study, we did not observe any laser Ag-TiO 2 NPs that were in the nuclei.…”

mentioning

confidence: 93%

Antibacterial mechanisms of a novel type picosecond laser-generated silver-titanium nanoparticles and their toxicity to human cells

Korshed¹,

Li²,

Li³

et al. 2017

IJN

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In this study, we explored the antibacterial mechanisms for a novel type of Ag-TiO 2 compound nanoparticles (NPs) produced from an Ag-TiO 2 alloy using a picosecond laser and evaluated the toxicity of the Ag-TiO 2 NPs to a range of human cell types. Transmission electron microscopy was used to determine the morphology, shapes, and size distribution of the laser-generated Ag-TiO 2 NPs. UV-visible spectrometer was used to confirm the shift of light absorbance of the NPs toward visible light wavelength. Results showed that the laser-generated Ag-TiO 2 NPs had significant antibacterial activities against both Gram-negative and Gram-positive bacterial strains, including Escherichia coli, Pseudomonas aeruginosa , and the methicillin-resistant Staphylococcus aureus . Increased level of reactive oxygen species was produced by E. coli after exposure to the Ag-TiO 2 NPs, which was accompanied with lipid peroxidation, glutathione depletion, disintegration of cell membrane and protein leakage, leading to the cell death. Five types of human cells originated from lung (A549), liver (HePG2), kidney (HEK293), endothelium cells (human coronary artery endothelial cells [hCAECs]), and skin (human dermal fibroblast cells [HDFc]) were used to evaluate the cytotoxicity of the laser-generated Ag-TiO 2 NPs. A weak but statistically significant decrease in cell proliferation was observed for hCAECs, A549 and HDFc cells when co-cultured with 2.5 µg/mL or 20 µg/mL of the laser-generated Ag-TiO 2 NPs for 48 hours. However, this effect was no longer apparent when a higher concentration of NPs (20 µg/mL) was used after 72 hours of co-culture with human cells, suggesting a possible adaptive process in the cells had occurred. We conclude that picosecond laser-generated Ag-TiO 2 NPs have a broad spectrum of antibacterial effect, including against the drug-resistant strain, with multiple underlying molecular mechanisms and low human cell toxicity. The antimicrobial properties of the new type of picoseconds laser-generated Ag-TiO 2 compound NPs could have potential biomedical applications.

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Targeted Charge‐Reversal Nanoparticles for Nuclear Drug Delivery

Cited by 359 publications

References 31 publications

Multicompartment Intracellular Self‐Expanding Nanogel for Targeted Delivery of Drug Cocktail

Multicompartment Intracellular Self‐Expanding Nanogel for Targeted Delivery of Drug Cocktail

Tumor-targeting, pH-sensitive nanoparticles for docetaxel delivery to drug-resistant cancer cells

Antibacterial mechanisms of a novel type picosecond laser-generated silver-titanium nanoparticles and their toxicity to human cells

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