Targeted delivery of chitosan nanoparticles to Peyer’s patch using M cell-homing peptide selected by phage display technique

Recombinant interleukin 6 with M cell-targeting moiety produced in Lactococcus lactis IL1403 as a potent mucosal adjuvant for peroral immunization

“…Closed-ileal loop and immunohistochemistry assays were conducted to verify M-cell targeting property of the recombinant IL-6s secreted from L. lactis IL1403 according to the method previously reported by our research group [14]. Briefly, 8 week-old female BalB/c mice (Samtako, Korea) were fasted overnight and anesthetized.…”

“…Thus, M cell-targeting might be one of the promising strategies to develop an effective oral vaccine [13]. An M celltargeting peptide ligand, CKS9, consisted of 9-amino acids with cyclic conformation was identified via phage display screening in our previous research [14]. In this study, we developed a novel M cell-targeting cytokine adjuvant system for oral vaccine by generation of recombinant L. lactis producing murine IL-6 conjugated with CKS9.…”

Fate of polymeric nanocarriers for oral drug delivery

“…3B). The grafting of new M cell homing peptides identified by phage display on nanoparticles is associated with increased delivery to M cells and enhanced transport [68,71]. Thiamine-coated nanoparticles show a strong capacity to be captured by Peyer's patches [23].…”

A novel ligand conjugated nanoparticles for oral insulin delivery

“…Among those intestinal epithelium cells, M cells are the mostly studied target because of their special physiological functions (Kunisawa et al, 2012). Some ligands such as lectins, ulexeuropaeus agglutinin 1, RGD and CKSTHPLSC peptide have been reported to target to different receptors of M cells (Clark et al, 2001;Zhang et al, 2005;Garinot et al, 2007;Yoo et al, 2010). However, the M cells account for only small number of intestinal cells and the targeting ligands usually were used to transport vaccine, thus limits their application.…”