Targeted Delivery of Doxorubicin in HeLa Cells Using Self‐Assembled Polymeric Nanocarriers Guided by Deep Eutectic Solvents

Abstract

Green material/solvents have been extensively investigated for biomedical applications, including drug delivery applications.In this work, the material design of deep eutectic solvents (DESs) grafted chitosan (CS) with biotin nanocarrier was synthesized from green solvents or deep eutectic solvents and evaluated for sustained anticancer drug release of doxorubicin (DOX) drug. The chemical structure and physical properties of the DESs, DES-g-CS-Biotin, and DOX loaded polymeric nanocarrier were investigated via NMR, FT-IR, and density analysis. The surface morphology and size of the polymeric nanocarrier were determinedby Atomic Force Microscopy (AFM), Scanning Electron Microscopy (SEM), and Transmission Electron Micro-scopy (TEM), and Dynamic Light Scattering (DLS) techniques. Furthermore, the sustained release of DOX loaded polymeric nanocarrier was studied using UV-visible spectroscopy at the λ max value of 325 nm. The sustained release of drug discharge from the polymeric nanocarrier was evaluated under various physiological conditions.Moreover, the cell viability and cytotoxicity effects against polymeric nanocarrier and DOX loaded polymeric nanocarrier were evaluated using VERO and HeLa cell lines. In conclusion, the polymeric nanocarrier, with deep eutectic solvent enhances the bioavailability, and biocompatibility to biological system improves cancer chemotherapy treatment.

Deep Eutectic Solvents — A New Method for Transdermal Drug Delivery

“…Интересные результаты представлены в работе P. Pradeepkumar и соавт. (2021) [43]. Авторы разработали ГЭР на основе серина и молочной кислоты, затем на полимерный носитель хитозан привили полученный растворитель и биотин.…”

Deep Eutectic Solvents: Are They Safe?

“…DESs have attracted the attention of the scientific community because of their potential applications. These mixtures can be used in organic synthesis, electrochemistry, extraction media [30][31][32][33][34], biotechnology or biodiesel [35][36][37] synthesis and separation processes [38,39], as a solvent in biological assays or enzymatic reactions [40], in biocatalysis [41,42], or in biomedical applications [43][44][45][46]. Additionally, they have been used in the pharmaceutical industry as excipients for increasing the solubility of hydrophobic drugs, or in drug delivery formulations [47][48][49][50][51].…”

Extractive Desulfurization of Liquid Fuel with Three-Body NMP/BEN/H2O Deep Eutectic Solvents

“…However, compared with ILs, DESs also have the advantages of a simple preparation process, cheap raw materials and easy availability, nontoxicity, and easy biodegradation. It is widely used in electrochemistry, , gas absorption, − synthesis of nanomaterials, − and drug release. , As an organic solvent, NMP is widely used in aromatic extraction, − lubricating oil antifreeze, extraction desulfurization of syngas and fuel oil, − etc. However, the application of DESs formed by its synthesis with BEN in EDS of fuel oil has rarely been reported, and the application of DESs in extractive desulfurization of fuel oil is currently limited to two-body low eutectic solvents.…”