Targeted delivery of paromomycin in murine infectious diseases through association to nano lipid systems

Copper Complex Nanoformulations Featuring Highly Promising Therapeutic Potential in Murine Melanoma Models

“…Nanoliposomes composed of the selected phospholipids were prepared by the dehydrationrehydration method [30,31]. Long circulating nanoliposomes were produced by including DSPE-PEG.…”

“…The transition temperature (Tc) of phospholipids was taken at the interception of the baseline with the tangents of the right and left side of the heating curve. The enthalpy of Tc was obtained from the area under the peaks and normalized to phospholipid content [31]. At the end of the incubation period, the separation of released Cuphen was performed by ultracentrifugation at 250,000×g, for 120 min, at 15°C in a Beckman LM-80 ultracentrifuge.…”

Nanoformulations of a Potent copper-based Aquaporin Inhibitor With Cytotoxic Effect Against Cancer Cells

“…The hemolytic activity of Cuphen in free and liposomal forms as well as in unloaded liposomes was determined using EDTA-preserved peripheral human blood [41] . Human peripheral blood, EDTA-preserved, was obtained from voluntary donors and used in the same day of experiments.…”

“…administration to test for hemolytic potential [61] . The in vitro hemolysis assay evaluates hemoglobin release in the plasma (as an indicator of red blood cell lysis) following exposure to a test agent [41] . In the present work, the hemolytic activity against RBCs was used as a marker of a general membrane toxicity effect of Cuphen.…”

Nanoliposomal artemisinin for the treatment of murine visceral leishmaniasis

“…9,10 Over the past decades, liposomes have demonstrated an increase in the therapeutic efficacy of several drugs. [11][12][13][14][15][16][17][18] Depending upon their physical properties, liposomes are recognized as foreign particles after in vivo administration and are easily cleared by the reticuloendothelial system that harbors Leishmania parasites. [19][20][21] Thus, the association or incorporation of chemotherapeutic agents in liposomes has immense therapeutic benefits against the intracellular infection of Leishmania.…”