Targeted Development-Dependent Metabolomics Profiling of Bioactive Compounds in Acanthopanax senticosus by UPLC-ESI-MS

Abstract

An ultra-performance liquid chromatography-electrospray ionization-mass spectrometry targeted metabolomics strategy was applied to analyze protocatechuate, syringin, eleutheroside E, isofraxidin, hyperoside, kaempferol, and oleanolic acid, the active compounds in 3-year-old, 5-year-old, and 9-year-old Acanthopanax senticosus. Then, targeted metabolomics was conducted with 3 growth year plants to identify 19 phenolic metabolites related to the above-mentioned active compounds, including 9 C6C3C6-type, 6 C6C3-type, and 4 C6C1-type. Multivariate statistical analysis was applied to the bioactive metabolite data, and targeted metabolic profiling was used for marker compound classification and characterization. The results showed that 7 active compounds in the roots and stems in the 3 growth year plants differed. The principal component “Q” values showed that the total contents of 7 active compounds in 5-year-old roots and stems were higher than in other growth years. Results of targeted metabolomics profiling of 19 phenolic metabolites showed that the C6C1-type compounds accumulated in 9-year-old plants, the C6C3-type in 3-year-old plants, and the C6C3C6-type in 5-year-old plants. The stems had the greatest accumulations of the phenolic metabolites. C6C1 and C6C3-type metabolites are the most abundant in both roots and stems. In conclusion, the active compounds and pharmacological effects of A. senticosus in different growth years are different. The best harvest age for A. senticosus roots and stems was 5 years. The accumulation of 19 phenolic metabolites in different growth years also showed significant differences.

Effects of light intensity on the growth, photosynthetic characteristics, and secondary metabolites of Eleutherococcus senticosus Harms

“…The targeted metabolic analytical conditions for 13 phenolic metabolites were the same as in our previous studies (Xu et al 2020). Samples were analyzed using an LC system coupled to a QTOF tandem-mass spectrometer via electrospray ionization (ESI) interface (Agilent 6520, Agilent Technologies, Santa Clara, CA, USA).…”

Mass Spectrometry-Based Nontargeted and Targeted Analytical Approaches in Fingerprinting and Metabolomics of Food and Agricultural Research

“…A metabolomic approach can be performed either in the nontargeted mode, targeted mode, or a combination of both, depending on the purposes of the research. − Targeted metabolomics focuses on a set of known specific metabolites, while nontargeted metabolomics is a comprehensive analysis focusing on all detectable metabolites to provide a metabolic overview . The targeted approach has the merit of providing reliable identification and accurate quantification. , However, prior knowledge of the metabolites is necessary for the successful application of the targeted MS-based approach. , Currently, “target analysis” regards not only the quantification of the absolute amounts of metabolites by using authentic standards but also the profiles of a specific class of metabolites from MS-based analysis, reference extracts, mutant analysis, data deconvolution, and web resources. ,− Most of the time, both nontargeted and targeted approaches could be applied to identify new marker compounds and complement each other. , …”

Tissue-Specific Metabolic Response of Acanthopanax Senticosus (Rupr. Et. Maxim.) Harms Leaf To UV-B radiation

“…Meanwhile, the widespread use of A. senticosus has gradually depleted resources, even causing an ecological crisis (Xu et al 2020). The consumption of A. senticosus root, the most common way, is increasingly replaced by its leaf due to the similar effect.…”