Targeted disruption of Rab1a causes early embryonic lethality

Abstract

Guanosine nucleotide diphosphate (GdP) dissociation inhibitor 2 (GdI2) regulates the GdP/guanosine triphosphate (GTP) exchange reaction of Rab proteins by inhibiting the dissociation of GdP and the subsequent binding of GTP. The present study aimed to determine the function of Rab1a in vivo, and thus generated mice with a trapped Rab1a gene. It was demonstrated that Rab1a is essential for embryonic development. It was also found that one functional Rab1a allele was sufficient for development in a heterozygous murine embryo, whereas a double mutant led to embryonic lethality. The dissection of uteri on embryonic day (E)10.5-14.5 yielded no homozygous embryos, indicating that homozygotes die between E10.5 to E11.5. The gene trap construct contains a β-galactosidase/neomycin reporter gene, allowing for heterozygotes to be stained for β-galactosidase to determine the tissue-specific expression of Rab1a. Rab1a was found to be highly expressed in the small intestine of both adult mice and embryos, although its expression levels were low in the brains of embryos. Moreover, there was no significant change in cytokine production and survival in wild-type and heterozygous Rab1a +/mice following a challenge with lipopolysaccharide. On the whole, the present study demonstrates that the disruption of the Rab1a gene causes embryonic lethality and homozygotes die between E10.5 and E11.5, suggesting that Rab1a is essential for the early development of mouse embryos.