Targeted gold nanoshelled hybrid nanocapsules encapsulating doxorubicin for bimodal imaging and near-infrared triggered synergistic therapy of Her2-positve breast cancer

Abstract

A multifunctional targeted nanoplatform combining photothermal therapy and chemotherapy has emerged as a promising strategy for comprehensive therapies of breast cancer. In this study, we constructed human epidermal growth factor receptor 2 (Her2)-targeted gold nanoshelled poly(lactic- co-glycolic acid) hybrid nanocapsules encapsulating perfluorooctyl bromide, superparamagnetic iron oxide nanoparticles, and doxorubicin (Her2-GPDH nanocapsules) as theranostic agent for bimodal ultrasound/magnetic resonance imaging and synergistic photothermal-chemotherapy of Her2-postive breast cancer cells. Her2–GPDH nanocomposites possessed well-defined spherical morphology, and the average diameter was about 296 nm with good dispersion. Targeting assays demonstrated that Her2–GPDH nanocapsules exhibited higher targeting binding to Her2-positive SKBR3 cells than Her2-negative MDA-MB-231cells. The encapsulation efficiency and the loading content of doxorubicin in Her2–GPDH nanocapsules were 39 ± 1.45% and 3.8 ± 0.52%, respectively, and the agent exhibited pH-responsive and near-infrared light-triggered stepwise release behavior of doxorubicin. In vitro, the agent had potential to serve as feasible candidate for ultrasound imaging and T2-weighted magnetic resonance imaging with a relatively high relaxivity. Cell experiments confirmed that the agent had significant photothermal cytotoxicity on SKBR3 cells, and the combined photothermal–chemotherapy could significantly enhance the anti-tumor effect. In summary, the present Her2–GPDH nanocapsules, a novel multifunctional nanoplatform, will offer a new way for early bimodal molecular-level diagnosis and synergistic treatment of Her2-positve breast cancer.

Application of Ultrasound Combined with Magnetic Resonance Imaging in the Diagnosis and Grading of Patients with Prenatal Placenta Accreta

“…From October 2019 to October 2021, 312 pregnant women who gave birth in our hospital and were diagnosed with placenta accreta by intraoperative clinical diagnosis and postoperative pathological diagnosis were selected as the research objects, the age ranged from 22 to 38 years, with an average of 26.98 ± 5.85 years old, the gestational age was 30 to 38 weeks, with an average of 35.69 ± 2.58 weeks, and the pregnancy was 1 to 5 times, with an average of 2.69 ± 2.69 ± 2.58 weeks. The parity was 1 to 4 times, with an average of 2.08 ± 0.87 times, 280 cases had a history of cesarean section, 20 cases had a history of myomectomy, and 12 cases had a history of other uterine cavity operations; all pregnant women were clearly conscious, without serious cardiovascular and cerebrovascular diseases, abnormal liver and kidney functions, coagulation disorders, and mental disorders [ 11 ].…”

Using anti‐PD‐L1 antibody conjugated gold nanoshelled poly (Lactic‐co‐glycolic acid) nanocapsules loaded with doxorubicin: A theranostic agent for ultrasound imaging and photothermal/chemo combination therapy of triple negative breast cancer

“…NCs were prepared by a carbodiimide method. 15 Briefly, 1 mL of the DOX@PLGA@Au NCs solution with a concentration of 2 mg/mL was taken and diluted appropriately with phosphate buffer saline (PBS), 5 mg of SH-PEG-COOH powder was added at the same time, and then it was uniformly dispersed by an ultrasonic cleaner. Then, the above mixture was stirred at a constant speed for 12 h at room temperature, and then centrifuged and washed twice (centrifugation conditions: 25155 g, 20 min, 15 C), and the obtained precipitate was finally DOX@PLGA@Au NCs linked to poly (ethylene glycol) (PEG).…”

“…4,5 Compared with free chemotherapeutic drugs, the PLGA drug-loading system has fewer toxic effects on normal tissues. 14,15 Gold nanoparticles (Au NPs) are biocompatible inorganic nanomaterials widely used as bioimaging and photothermal conversion agents owing to their unique optical and surface plasmon resonance (SRP) properties. 16 Ke, et al constructed a surface-loaded gold nanoshell structure and performed ultrasound imaging and photothermal therapy using a nude mouse model.…”

SPIONs Conjugate Supported Anticancer Drug Doxorubicin’s Delivery: Current Status, Challenges, and Prospects

“…The DOX displayed considerable photothermal cytotoxicity on the SKBR3 cells. According to the study, due to the combination therapy consisting of photothermal applications in conjunction with chemotherapy, the antitumor impacts significantly improved [ 40 ].…”