Targeted imaging of activated caspase-3 in the central nervous system by a dual functional nano-device

Liu, Yang; Hu, Yuzhi; Guo, Yubo; Ma, Haojun; Li, Jianfeng; Jiang, Chen

doi:10.1016/j.jconrel.2012.09.001

Cited by 23 publications

(24 citation statements)

References 34 publications

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“…1C ). In our previous study [23], the principal organs including brain, heart, liver, spleen, lung and kidney were removed for ex vivo imaging analysis. The signals were mainly accumulated in liver, spleen and kidney.…”

Section: Resultsmentioning

confidence: 99%

Targeting Caspase-3 as Dual Therapeutic Benefits by RNAi Facilitating Brain-Targeted Nanoparticles in a Rat Model of Parkinson’s Disease

Liu

Guo

et al. 2013

PLoS ONE

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The activation of caspase-3 is an important hallmark in Parkinson’s disease. It could induce neuron death by apoptosis and microglia activation by inflammation. As a result, inhibition the activation of caspase-3 would exert synergistic dual effect in brain in order to prevent the progress of Parkinson’s disease. Silencing caspase-3 genes by RNA interference could inhibit the activation of caspase-3. We developed a brain-targeted gene delivery system based on non-viral gene vector, dendrigraft poly-L-lysines. A rabies virus glycoprotein peptide with 29 amino-acid linked to dendrigraft poly-L-lysines could render gene vectors the ability to get across the blood brain barrier by specific receptor mediated transcytosis. The resultant brain-targeted vector was complexed with caspase-3 short hairpin RNA coding plasmid DNA, yielding nanoparticles. In vivo imaging analysis indicated the targeted nanoparticles could accumulate in brain more efficiently than non-targeted ones. A multiple dosing regimen by weekly intravenous administration of the nanoparticles could reduce activated casapse-3 levels, significantly improve locomotor activity and rescue dopaminergic neuronal loss and in Parkinson’s disease rats’ brain. These results indicated the rabies virus glycoprotein peptide modified brain-targeted nanoparticles were promising gene delivery system for RNA interference to achieve anti-apoptotic and anti-inflammation synergistic therapeutic effects by down-regulation the expression and activation of caspase-3.

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Section: Resultsmentioning

confidence: 99%

Targeting Caspase-3 as Dual Therapeutic Benefits by RNAi Facilitating Brain-Targeted Nanoparticles in a Rat Model of Parkinson’s Disease

Liu

Guo

et al. 2013

PLoS ONE

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“…115 To achieved this goal, the authors designed and synthesized a brain-targeted nano-device, in which a biodegradable synthetic polymer, dendrigraft poly-L-lysines (DGLs) was used as a scaffold. The FQ pair (Cy5 and QSY-21) and a nine residue caspase-3 preferred sequence were attached to the DGLs.…”

Section: Caspase Substratesmentioning

confidence: 99%

Small Molecule Active Site Directed Tools for Studying Human Caspases

et al. 2015

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Caspases are proteases of clan CD and were described for the first time more than two decades ago. They play critical roles in the control of regulated cell death pathways including apoptosis and inflammation. Due to their involvement in the development of various diseases like cancer, neurodegenerative diseases or autoimmune disorders, caspases have been intensively investigated as potential drug targets, both in academic and industrial laboratories. This review presents a thorough, deep, and systematic assessment of all technologies developed over the years for the investigation of caspase activity and specificity using substrates and inhibitors, as well as activity based probes, which in recent years have attracted considerable interest due to their usefulness in the investigation of biological functions of this family of enzymes.

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“…When associated with ap eptidic ligand for avb3i ntegrin receptors (i.e.,R GD), the nanoparticles permitted in vivo magnetic resonance imagingo fl iving mice bearing HepG2 tumours. [49] Such approaches minimize the background signalf or increased specificity and sensitivity of the images. [47] In some cases,t he opticals ignal may also be turned on by desired events, such as ap H-dependant electron transfer between the fluorophore and a quencher, [48] or the separation of the fluorophore and a quencher upon enzymatic cleavage.…”

Section: Imagingmentioning

confidence: 99%

“…[47] In some cases,t he opticals ignal may also be turned on by desired events, such as ap H-dependant electron transfer between the fluorophore and a quencher, [48] or the separation of the fluorophore and a quencher upon enzymatic cleavage. [49] Such approaches minimize the background signalf or increased specificity and sensitivity of the images.…”

Section: Imagingmentioning

confidence: 99%

Everything You Always Wanted to Know about Poly‐l‐lysine Dendrigrafts (But Were Afraid to Ask)

Francoïa

Vial

2018

Chemistry A European J

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Less than a decade ago, dendrigrafts of poly-l-lysine (DGLs) joined the family of polycationic dendritic macromolecules. Resulting from the iterative polycondensation of an N-carboxyanhydride in water, four generations of the dendrigraft can be obtained on a multigram scale and without chromatographic purification. DGLs share features with both dendrimers and hyperbranched polymers, but turned out to have unique biophysical and bioactive properties. The macromolecules-in their native form or functionalized-have been extensively characterized by various analytical and computational methods, and have already found numerous applications in the biomedical field, such as drug and gene delivery, biomaterials, tissue engineering, bioimaging, and biosensing. Despite a growing interest for DGLs, there is still plenty of room for further exciting developments that could result from a better exposure of these macromolecules, which is the ambition of this short review.

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Targeted imaging of activated caspase-3 in the central nervous system by a dual functional nano-device

Cited by 23 publications

References 34 publications

Targeting Caspase-3 as Dual Therapeutic Benefits by RNAi Facilitating Brain-Targeted Nanoparticles in a Rat Model of Parkinson’s Disease

Targeting Caspase-3 as Dual Therapeutic Benefits by RNAi Facilitating Brain-Targeted Nanoparticles in a Rat Model of Parkinson’s Disease

Small Molecule Active Site Directed Tools for Studying Human Caspases

Everything You Always Wanted to Know about Poly‐l‐lysine Dendrigrafts (But Were Afraid to Ask)

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