Targeted lipidomics reveals changes in N-acyl serines by acute exposure to an electric field: Molecular insights into the docking of N-18:1 serine interaction with TRPV1 or PPAR-α

Abstract

Alternative therapy with medical devices using high-voltage electric potential (HELP) to generate an electric field (EF) is common in Japan. However, the mechanisms underlying potential health benefits of this therapy remain unclear. Therefore, we investigated the effect of HELP exposure (9 kV /electrode + 9 kV/electrode, 30 min) on N-acyl serines (N-acyl SERs) using selected reaction monitoring (SRM) analysis in plasma samples obtained from healthy human subjects before and after a single treatment session.

Molecular insights into the docking of N-palmitoyl serine (Palmi-Ser) interaction with the nuclear receptor subfamily 4 group A member 2 (NR4A2): Effect of Palmi-Ser on survival rate in the Drosophila model of Parkinson's disease

“…The cells treated with vehicle (0.1% ethanol) were used as the control. qRT-PCR was conducted as previously described [8,11,15]. Total RNA isolation and TaqMan-based qRT-PCR was conducted using a FastLane cell probe kit (Qiagen, Valencia, CA, USA) in a Light Cyler 96 system (Roche Applied Science, Mannheim, Germany).…”

“…High-voltage electric field (EF) therapy may be effective in treating shoulder stiffness, headache, insomnia, and chronic constipation [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15]. Although EF therapy was discovered about 90 years ago, the molecular mechanisms associated with its health benefits remain elusive.…”

“…Although EF therapy was discovered about 90 years ago, the molecular mechanisms associated with its health benefits remain elusive. The Ministry of Health, Labor, and Welfare in Japan approved a therapeutic device that exposes the human body to high-voltage electric potential (HELP) [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15]. A review of the literature suggests that HELP exposure may be an alternative therapy for several conditions.…”

“…A review of the literature suggests that HELP exposure may be an alternative therapy for several conditions. It was previously reported that HELP exposure-induced upregulation of Palmi-Ser, Oleo-Ser, oleoylethanolamide, 9-hydroxyoctadecadienoic acid (HODE), 13-HODE, lysophosphatidylethanolamine-20:4 (LysoPE-20:4), lysophosphatidylethanolamine-22:6 (LysoPE-22:6), and lysophosphatidylcholine-22:4 (LysoPC-22:4) levels in the plasma of healthy individuals using metabolome analysis [10,12,13,15]. Endogenous lipid-derived metabolites have been suggested as candidate molecules representing the interface between symptoms and electroceutical target proteins [8][9][10][11][12][13][14][15].…”

“…It was previously reported that HELP exposure-induced upregulation of Palmi-Ser, Oleo-Ser, oleoylethanolamide, 9-hydroxyoctadecadienoic acid (HODE), 13-HODE, lysophosphatidylethanolamine-20:4 (LysoPE-20:4), lysophosphatidylethanolamine-22:6 (LysoPE-22:6), and lysophosphatidylcholine-22:4 (LysoPC-22:4) levels in the plasma of healthy individuals using metabolome analysis [10,12,13,15]. Endogenous lipid-derived metabolites have been suggested as candidate molecules representing the interface between symptoms and electroceutical target proteins [8][9][10][11][12][13][14][15]. Furthermore, defining the molecular interaction between endogenous metabolites and target proteins is essential for understanding the unknown pharmacological effects of human metabolites [16][17][18][19].…”