Targeted magnetic delivery and tracking of cells using a magnetic resonance imaging system

Riegler, Johannes; Wells, Jack A.; Kyrtatos, Panagiotis G.; Price, Anthony N.; Pankhurst, Quentin A.; Lythgoe, Mark F.

doi:10.1016/j.biomaterials.2010.03.032

Cited by 112 publications

(123 citation statements)

References 43 publications

Supporting

Mentioning

123

Contrasting

Order By: Relevance

“…To overcome this problem, various technical setups have been proposed that (among others) include custom-made electromagnets, the use of an arrangement of permanent magnets, the implantation of a magnetizable material, or the design of special gradient coils for conventional MRI scanners. [32][33][34][35][36] In general, the attractive force exerted on a particle by a magnetic targeting system should equal the hydrodynamic …”

Section: Essential Requirements For Magnetic Targeting Of Ionsmentioning

confidence: 99%

Remote magnetic targeting of iron oxide nanoparticles for cardiovascular diagnosis and therapeutic drug delivery: where are we now?

Bietenbeck¹,

Florian²,

Faber³

et al. 2016

IJN

View full text Add to dashboard Cite

Magnetic resonance imaging (MRI) allows for an accurate assessment of both functional and structural cardiac parameters, and thereby appropriate diagnosis and validation of cardiovascular diseases. The diagnostic yield of cardiovascular MRI examinations is often increased by the use of contrast agents that are almost exclusively based on gadolinium compounds. Another clinically approved contrast medium is composed of superparamagnetic iron oxide nanoparticles (IONs). These particles may expand the field of contrast-enhanced cardiovascular MRI as recently shown in clinical studies focusing on acute myocardial infarction (AMI) and atherosclerosis. Furthermore, IONs open up new research opportunities such as remote magnetic drug targeting (MDT). The approach of MDT relies on the coupling of bioactive molecules and magnetic nanoparticles to form an injectable complex. This complex, in turn, can be attracted to and retained at a desired target inside the body with the help of applied magnetic fields. In comparison to common systemic drug applications, MDT techniques promise both higher concentrations at the target site and lower concentrations elsewhere in the body. Moreover, concurrent or subsequent MRI can be used for noninvasive monitoring of drug distribution and successful delivery to the desired organ in vivo. This review does not only illustrate the basic conceptual and biophysical principles of IONs, but also focuses on new research activities and achievements in the cardiovascular field, mainly in the management of AMI. Based on the presentation of successful MDT applications in preclinical models of AMI, novel approaches and the translational potential of MDT are discussed.

show abstract

Section: Essential Requirements For Magnetic Targeting Of Ionsmentioning

confidence: 99%

Remote magnetic targeting of iron oxide nanoparticles for cardiovascular diagnosis and therapeutic drug delivery: where are we now?

Bietenbeck¹,

Florian²,

Faber³

et al. 2016

IJN

View full text Add to dashboard Cite

show abstract

“…The tubes were filled with 50 ml of complete medium containing a suspension of 1 Â 10 6 L929 cells pre-incubated with medium containing 125 mg ml 21 SPION for 24 h. The tubes were loaded into either the rotating Halbach cylinder and incubated for 2 h at 378C 5 per cent CO 2 or placed horizontally onto a support and incubated at 378C for a total of 2 h, with 908 rotation every 30 min, as outlined already. After incubation, the culture medium was removed and the lumen of the jejunum gently washed once with PBS to remove unattached cells, followed by fixation in 2 per cent formaldehyde solution for 24 h. [14]. The cross section of the tubes was divided into four segments.…”

Section: Application Of the Halbach Cylinder Device To Gastrointestinmentioning

confidence: 99%

Rapid magnetic cell delivery for large tubular bioengineered constructs

Gonzalez‐Molina

Riegler

Southern

et al. 2012

J. R. Soc. Interface.

View full text Add to dashboard Cite

Delivery of cells into tubular tissue constructs with large diameters poses significant spatial and temporal challenges. This study describes preliminary findings for a novel process for rapid and uniform seeding of cells onto the luminal surface of large tubular constructs. Fibroblasts, tagged with superparamagnetic iron oxide nanoparticles (SPION), were directed onto the luminal surface of tubular constructs by a magnetic field generated by a k4-type Halbach cylinder device. The spatial distribution of attached cells, as measured by the mean number of cells, was compared with a conventional, dynamic, rotational cell-delivery technique. Cell loading onto the constructs was measured by microscopy and magnetic resonance imaging. The different seeding techniques employed had a significant effect on the spatial distribution of the cells ( p , 0.0001). The number of attached cells at defined positions within the same construct was significantly different for the dynamic rotation technique ( p , 0.05). In contrast, no significant differences in the number of cells attached to the luminal surface were found between the defined positions on the construct loaded with the Halbach cylinder. The technique described overcomes limitations associated with existing cell-delivery techniques and is amenable to a variety of tubular organs where rapid loading and uniform distribution of cells for therapeutic applications are required.

show abstract

“…22 We have also shown that SPION-loaded MSCs can be targeted to specific areas via magnetic targeting. [31][32][33] We have therefore hypothesized that the tumor-targeting potential of MSCs could be exploited to deliver SPIONs to tumors for subsequent hyperthermia therapy. However, we have not previously established whether SPIONs within the MSCs degrade or otherwise lose their heating potential during the period between their introduction into the body and their subsequent arrival at the tumor.…”

Section: Introductionmentioning

confidence: 99%

Hyperthermia treatment of tumors by mesenchymal stem cell-delivered superparamagnetic iron oxide nanoparticles

Kalber

Ordidge

Southern³

et al. 2016

IJN

View full text Add to dashboard Cite

Magnetic hyperthermia – a potential cancer treatment in which superparamagnetic iron oxide nanoparticles (SPIONs) are made to resonantly respond to an alternating magnetic field (AMF) and thereby produce heat – is of significant current interest. We have previously shown that mesenchymal stem cells (MSCs) can be labeled with SPIONs with no effect on cell proliferation or survival and that within an hour of systemic administration, they migrate to and integrate into tumors in vivo. Here, we report on some longer term (up to 3 weeks) post-integration characteristics of magnetically labeled human MSCs in an immunocompromized mouse model. We initially assessed how the size and coating of SPIONs dictated the loading capacity and cellular heating of MSCs. Ferucarbotran ® was the best of those tested, having the best like-for-like heating capability and being the only one to retain that capability after cell internalization. A mouse model was created by subcutaneous flank injection of a combination of 0.5 million Ferucarbotran-loaded MSCs and 1.0 million OVCAR-3 ovarian tumor cells. After 2 weeks, the tumors reached ~100 µL in volume and then entered a rapid growth phase over the third week to reach ~300 µL. In the control mice that received no AMF treatment, magnetic resonance imaging (MRI) data showed that the labeled MSCs were both incorporated into and retained within the tumors over the entire 3-week period. In the AMF-treated mice, heat increases of ~4°C were observed during the first application, after which MRI indicated a loss of negative contrast, suggesting that the MSCs had died and been cleared from the tumor. This post-AMF removal of cells was confirmed by histological examination and also by a reduced level of subsequent magnetic heating effect. Despite this evidence for an AMF-elicited response in the SPION-loaded MSCs, and in contrast to previous reports on tumor remission in immunocompetent mouse models, in this case, no significant differences were measured regarding the overall tumor size or growth characteristics. We discuss the implications of these results on the clinical delivery of hyperthermia therapy to tumors and on the possibility that a preferred therapeutic route may involve AMF as an adjuvant to an autologous immune response.

show abstract

Targeted magnetic delivery and tracking of cells using a magnetic resonance imaging system

Cited by 112 publications

References 43 publications

Remote magnetic targeting of iron oxide nanoparticles for cardiovascular diagnosis and therapeutic drug delivery: where are we now?

Remote magnetic targeting of iron oxide nanoparticles for cardiovascular diagnosis and therapeutic drug delivery: where are we now?

Rapid magnetic cell delivery for large tubular bioengineered constructs

Hyperthermia treatment of tumors by mesenchymal stem cell-delivered superparamagnetic iron oxide nanoparticles

Contact Info

Product

Resources

About