Targeted mapping of a sugarcane rust resistance gene (Bru1) using bulked segregant analysis and AFLP markers

Asnaghi, Carole; Roques, Danièle; Ruffel, Sandrine; Kaye, Claudia; Hoarau, Jean-Yves; Telismart, Hugues; Girard, Jean-Claude; Raboin, Louis-Marie; Risterucci, Ange-Marie; Grivet, Laurent; D’Hont, Angélique

doi:10.1007/s00122-003-1487-6

Cited by 158 publications

(152 citation statements)

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“…The advantage of this approach is that the likelihood of identifying false positive markers is very small (Michelmore et al 1991, Meksem et al 1995, Li et al 1998. The combination of the AFLP technique and BSA has been effectively used to generate molecular markers that are closely linked to the genes of interest in many crops (Chantret et al 2000, Miftahudin et al 2002, Asnaghi et al 2004. In most of these studies, F 2 populations were used to establish pools (Gallego et al 1998), however, the dominant male-sterile gene results in a F 1 population with 100% male-sterile plants.…”

Section: Discussionmentioning

confidence: 99%

Identification of AFLP markers linked to Ms, a genic multiple allele inherited male-sterile gene in Chinese cabbage

et al. 2009

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As a first step toward cloning the genic multiple allele inherited male-sterile gene Ms in Chinese cabbage (Brassica rapa L. ssp pekinensis (Lour.) Olsson), we attempted the molecular tagging of this male-sterility locus using the amplified fragment length polymorphism (AFLP) technique. A BC 1 mapping population (130 plants) segregating for male sterility/fertility was constructed for the tagging using the bulked segregant analysis (BSA) method. Screening 144 primer combinations, four AFLP markers were identified to be tightly linked to the Ms gene, the nearest one of which, AFLP01, was converted to a SCAR marker for the markerassisted selection (MAS). Finally, a genetic map of the Ms gene was constructed with four AFLP markers and one SCAR marker, covering a total interval of 13.9 cM. Two markers AFLP01(SCAR01) and AFLP04 flanked the Ms locus at distances of 2.3 and 7.8 cM, respectively.

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Section: Discussionmentioning

confidence: 99%

Identification of AFLP markers linked to Ms, a genic multiple allele inherited male-sterile gene in Chinese cabbage

et al. 2009

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“…Bru1 is a major gene for resistance to brown rust of sugarcane [25]. It is important for sugarcane breeders to develop a database of parental clones using agronomic and physiological traits and molecular markers for improving disease resistance and for sustaining yields and profits [19].…”

Section: Bru1 In Parental Clones and Rust Rating In Their Progenymentioning

confidence: 99%

“…Therefore, the opportunity exists to utilize Bru1 in marker-assisted breeding and selection in order to improve brown rust resistance in sugarcane [29]. Bru1 has been used as a marker to identify if sugarcane genotypes are potentially resistant to brown rust [25,30,31]. Since 2009, this marker has also been utilized in the CP program to direct breeding strategies for brown rust resistance [29].…”

Section: Bru1 In Parental Clones and Rust Rating In Their Progenymentioning

confidence: 99%

Screening for Sugarcane Brown Rust in the First Clonal Stage of the Canal Point Sugarcane Breeding Program

Zhao

Davidson²,

Baltazar³

et al. 2015

Agronomy

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Sugarcane (Saccharum spp.) brown rust (caused by Puccinia melanocephala Syd. & P. Syd.) was first reported in the United States in 1978 and is still one of the great challenges for sugarcane production. A better understanding of sugarcane genotypic variation in response to brown rust will help optimize breeding and selection strategies for disease resistance. Brown rust ratings were scaled from non-infection (0) to severe infection (4) with intervals of 0.5 and routinely recorded for genotypes in the first clonal selection stage of the Canal Point sugarcane breeding program in Florida. Data were collected from 14,272 and 12,661 genotypes and replicated check cultivars in 2012 and 2013, respectively. Mean rust rating, % infection, and severity in each family and progeny of female parent were determined, and their coefficients of variation (CV) within and among families (females) were estimated. Considerable variation exists in rust ratings among families or females. The families and female parents with high susceptibility or resistance to brown rust were identified and ranked. The findings of this study can help scientists to evaluate sugarcane crosses and parents for brown rust disease, to use desirable parents for crossing, and to improve genetic resistance to brown rust in breeding programs. OPEN ACCESSAgronomy 2015, 5 342

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“…Sydow., está presente em quase todas as áreas de cultivo (18,19,20). Em variedades suscetíveis, o patógeno prejudica a taxa fotossintética e vias correlatas, ocasionando reduções na altura e diâmetro dos colmos e na quantidade de perfilhos, comprometendo a produção final de biomassa (19,21,22,23).…”

Section: Introductionunclassified

“…A base da herança genética de resistência a essa doença tem sido amplamente estudada e alguns autores defendem que a resistência é controlada por um ou poucos genes (18,25,26,27,28,29,30,31,32,33). Um gene de resistência, denominado Bru1, foi o primeiro caráter mendeliano descrito para o complexo genoma da cana-de-açúcar (18,26,30,32).…”

Section: Introductionunclassified

Validação de marcadores moleculares associados à resistência à ferrugem marrom em cana-de-açúcar

et al. 2017

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A ferrugem marrom causada pelo fungo Puccinia melanocephala é uma importante doença em cana-de-açúcar (Saccharum spp.) e está presente em praticamente todas as áreas de cultivo. Um gene de efeito maior, Bru1, foi descrito como fonte de resistência durável da cana-de-açúcar contra isolados de P. melanocephala. O objetivo deste estudo foi avaliar a eficiência de dois marcadores moleculares fortemente associados ao gene Bru1, R12H16 e 9O20-F4-RsaI, para predição da resistência genética à ferrugem marrom em variedades de cana-de-açúcar. ABSTRACTBrown rust caused by the fungus Puccinia melanocephala is an important disease affecting sugarcane (Saccharum spp.) and is present in almost all growing areas. A major effect gene, Bru1, was described as a durable resistance source in sugarcane against isolates of P. melanocephala. The aim of this study was to evaluate the efficiency of two molecular markers strongly associated with Bru1 gene, R12H16 and 9O20-F4-RsaI, for prediction of genetic resistance to brown rust in sugarcane varieties. Thus, this study included 14 RB varieties, developed by RIDESA (Interuniversity Network for the Development of the Sugarcane Industry), which were among the 10 most cultivated varieties in the central-south region of Brazil in the period of 1974-2015. To evaluate the disease in the field an experiment was conducted in randomized blocks with four replicates. The severity of Barreto, F.Z.; Balsalobre, T.W.A. Chapola, R.G.; Hoffmann, H.P.; Carneiro, M.S. Validation of molecular markers associated with brown rust resistance in sugarcane. Summa Phytopathologica, v.43, n.1, p.36-40, 2017. brown rust was quantified according to a diagrammatic scale ranging from 1 (most resistant) to 9 (most susceptible), and the obtained data were analyzed by using a generalized linear mixed model (GLMM). Concomitantly, total DNA was extracted from the 14 varieties, followed by amplification of the molecular markers, which were classified into haplotypes. Out of the total varieties, 12 had no symptoms of the disease and showed 99.35% probability of being resistant according to analysis via GLMM; among them, 91.66% were haplotype 1, i.e., had the presence of both markers associated with Bru1 gene. Therefore, we can conclude that the molecular markers R12H16 and 9O20-F4-RsaI were highly efficient in predicting resistant phenotypes and that Bru1 gene is probably the main source of sugarcane resistance to brown rust in commercial fields in Brazil.Keywords: Saccharum spp., breeding, Puccinia melanocephala, Bru1, disease resistance.A cana-de-açúcar (Saccharum spp.) está entre as mais importantes espécies cultivadas, principalmente para produção de açúcar e bioetanol (1). O Brasil é atualmente o país com a maior produção de cana-deaçúcar com mais de nove milhões de hectares cultivados e produtividade estimada em 76.909 kg ha -1 na safra 2015/16 (1, 2). A cana-de-açúcar é uma gramínea semi-perene com propagação vegetativa, sendo que os programas de melhoramento genético têm concentrado esforços para o desenvolviment...

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Targeted mapping of a sugarcane rust resistance gene (Bru1) using bulked segregant analysis and AFLP markers

Cited by 158 publications

References 31 publications

Identification of AFLP markers linked to Ms, a genic multiple allele inherited male-sterile gene in Chinese cabbage

Identification of AFLP markers linked to Ms, a genic multiple allele inherited male-sterile gene in Chinese cabbage

Screening for Sugarcane Brown Rust in the First Clonal Stage of the Canal Point Sugarcane Breeding Program

Validação de marcadores moleculares associados à resistência à ferrugem marrom em cana-de-açúcar

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