Targeted metabolomics and mass spectrometry

Metabolomics of Medicinal Plants - A Versatile Tool for Standardization of Herbal Products and Quality Evaluation of Ayurvedic Formulations

“…Metabolomic fingerprinting can be very helpful in the field of herbal medicine for drug discovery, systems biology, gene-function analysis and various diagnostic techniques through different modern hyphenated technologies. A study involved in the characterization of a set of defined metabolites is known as 'targeted' metabolomics and usually combine NMR-MS techniques, which is applied for such types of analysis 16 . In this targeted metabolomics approaches, the relative concentration of approximately 200 predetermined metabolites is detected in a sample.…”

The Path to Personalized Cardiovascular Medicine

“…Both these technologies can be applied to analyze either a predefined subset of metabolites, structurally related or associated with a certain metabolic pathway -targeted metabolomics, or the entire set of metabolites -untargeted global metabolomics (Figure 24.8). 261,[319][320][321] Advanced NMR-and MS-based technologies have been initiated to use in profiling metabolomic changes associated with various CVD ranging from atherosclerosis, AF, and diabetes mellitus to pulmonary hypertension, MI, and HF. 319,320,[322][323][324] Several noninvasive, 1 H-NMR-based metabolomic studies have been applied to assess the severity of CAD; however, they have reached the conclusion that this approach is poor for predicting disease risk compared to standard angiography or clinical measurements of lipids and lipoproteins.…”

LC–MS-based metabolomics in the clinical laboratory

“…Analyses are performed predominantly qualitatively by determining a subset of the whole metabolite profile. Quadrupole/timeof-flight (QTOF), orbitrap and Fourier transform ion cyclotron resonance mass spectrometry systems are applied for the identification of metabolites in global metabolomic approaches [15][16][17][18][19][20]. Ion mobility time-of-flight mass spectrometry (IM-MS), which separates ions on the basis of their mass/charge ratios as well as their interactions with a buffer gas, becomes a powerful analytical tool for investigating complex samples.…”