Targeted metabolomics of CSF in healthy individuals and patients with secondary progressive multiple sclerosis using high-resolution mass spectrometry

Carlsson, Henrik; Abujrais, Sandy; Herman, Stephanie; Khoonsari, Payam Emami; Åkerfeldt, Torbjörn; Svenningsson, Anders; Burman, Joachim; Kultima, Kim

doi:10.1007/s11306-020-1648-5

Cited by 21 publications

(18 citation statements)

References 27 publications

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“…A global overview of acylcarnitine and triglyceride levels in plasma was obtained by targeted mass spectrometry–based metabolomics using the AbsoluteIDQ p400 HR Kit (Biocrates Life Sciences AG, Innsbruck, Austria) and performed by Biocrates according to the manufacturer’s instructions as described previously ( 52 ). Data were analyzed with the Biocrates MetIDQ software.…”

Section: Methodsmentioning

confidence: 99%

Remodeling of metabolism and inflammation by exercise ameliorates tumor-associated anemia

et al. 2021

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A considerable number of patients with cancer suffer from anemia, which has detrimental effects on quality of life and survival. The mechanisms underlying tumor-associated anemia are multifactorial and poorly understood. Therefore, we aimed at systematically assessing the patho-etiology of tumor-associated anemia in mice. We demonstrate that reduced red blood cell (RBC) survival rather than altered erythropoiesis is driving the development of anemia. The tumor-induced inflammatory and metabolic remodeling affect RBC integrity and augment splenic phagocyte activity promoting erythrophagocytosis. Exercise training normalizes these tumor-associated abnormal metabolic profiles and inflammation and thereby ameliorates anemia, in part, by promoting RBC survival. Fatigue was prevented in exercising tumor-bearing mice. Thus, exercise has the unique potential to substantially modulate metabolism and inflammation and thereby counteracts pathological remodeling of these parameters by the tumor microenvironment. Translation of this finding to patients with cancer could have a major impact on quality of life and potentially survival.

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Section: Methodsmentioning

confidence: 99%

Remodeling of metabolism and inflammation by exercise ameliorates tumor-associated anemia

et al. 2021

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“…High‐resolution mass spectrometry (HRMS) employs a sophisticated system with hybrid mass analyzers that greatly enhance the accuracy, precision, and reliability for compound determination. Recent studies show HRMS capability in valuable but low‐abundant biological molecular detection, and HRMS provides stability across batch‐to‐batch analysis (Carlsson et al, 2020; Zhu et al, 2021). HRMS application gained wide recognition due to its comprehensive and powerful technical characteristics in small biomolecule analysis.…”

Section: Introductionmentioning

confidence: 99%

An observation study of urinary biomarker exploratory in Alzheimer's disease using high‐resolution mass spectrometry

Zhang¹,

Wu²,

Liu

et al. 2022

Biomedical Chromatography

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Alzheimer's disease (AD) is regarded as a progressive neurodegenerative dementia, characterized by degeneration of distinct neuronal populations. A case–control study was carried out using high‐resolution mass spectrometry to explore AD‐associated urinary metabolic biomarkers from 30 AD patients and 30 cognitively normal (CN) individuals. In total, 49 metabolites were determined and validated as known compounds using LC/MS analysis. Using the two‐sample t‐test statistical analysis (P < 0.05), 19 metabolites were shown to be significantly different from AD to CN. A diagnostic model of the receiver operating characteristic curve was constructed with a combination of nine molecules out of 19 metabolites, it yielded a separation with an area under the curve value of 0.976 between the two groups. This study indicated that urinary metabolites showed a significant expression between AD and CN. AD‐related metabolites enable to satisfy the diagnostic power of disease discrimination. In addition, as a noninvasive approach, urine collection is done easily in clinical diagnosis of AD.

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“…The novelty of our study is the demonstration that changes in the brain metabolome were related to WBM induced changes in the fecal microbiome. The diet effect of feeding WBM on specific regions of the brain may be related to the sensitivity of the brain to circulating metabolites in the serum moving across the blood-brain barrier to alter the brain metabolic profile [24,68]. Lipidomic profiles in the serum can differ from the tissues such as the liver, brain and heart [69].…”

Section: Discussionmentioning

confidence: 99%

The Effects of Consuming White Button Mushroom Agaricus bisporus on the Brain and Liver Metabolome Using a Targeted Metabolomic Analysis

et al. 2021

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A targeted metabolomic analysis was performed on tissues derived from pigs fed diets supplemented with white button mushrooms (WBM) to determine the effect on the liver and brain metabolome. Thirty-one pigs were fed a grower diet alone or supplemented with either three or six servings of freeze-dried WBM for six weeks. Tissue metabolomes were analyzed using targeted liquid chromatography-mass spectrometry (LC-MS) combined with chemical similarity enrichment analysis (ChemRICH) and correlated to WBM-induced changes in fecal microbiome composition. Results indicated that WBM can differentially modulate metabolites in liver, brain cortex and hippocampus of healthy pigs. Within the glycero-phospholipids, there was an increase in alkyl-acyl-phosphatidyl-cholines (PC-O 40:3) in the hippocampus of pigs fed six servings of WBM. A broader change in glycerophospholipids and sphingolipids was detected in the liver with a reduction in several lipid species in pigs fed both WBM diets but with an increase in amino acids known as precursors of neurotransmitters in the cortex of pigs fed six servings of WBM. Metabolomic changes were positively correlated with increased abundance of Cryomorphaceae, Lachnospiraceae, Flammeovirgaceae and Ruminococcaceae in the microbiome suggesting that WBM can also positively impact tissue metabolite composition.

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Targeted metabolomics of CSF in healthy individuals and patients with secondary progressive multiple sclerosis using high-resolution mass spectrometry

Cited by 21 publications

References 27 publications

Remodeling of metabolism and inflammation by exercise ameliorates tumor-associated anemia

Remodeling of metabolism and inflammation by exercise ameliorates tumor-associated anemia

An observation study of urinary biomarker exploratory in Alzheimer's disease using high‐resolution mass spectrometry

The Effects of Consuming White Button Mushroom Agaricus bisporus on the Brain and Liver Metabolome Using a Targeted Metabolomic Analysis

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