Targeted mining of drought stress-responsive genes from EST resources in Cleistogenes songorica

Zhang, Jiyu; John, Ulrik P.; Wang, Yanrong; Li, Xi; Gunawardana, Dilini; Polotnianka, Renata M.; Spangenberg, Germán; Zhang, Nan

doi:10.1016/j.jplph.2011.04.005

Cited by 20 publications

(13 citation statements)

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“…In particular, Cleistogenes songorica was dominant at site 3. This species has a high drought tolerance and is common in Mongolian desert steppe (Zhang et al, 2011). However, site 3 is close to sites 4, 5 and 6 (Fig.…”

Section: Discussionmentioning

confidence: 87%

Community Structure and Productivity in Western Mongolian Steppe

2014

Mong. J. Biol. Sci.

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Cite this paper as:The people of the Mongolian steppe have maintained a sustainable, nomadic lifestyle. However, several ecological processes are threatening their way of life. Ecological changan be detected through the analysis of quantitative and qualitative data. It is therefore, imperative to develop a sustainable rangeland management system aimed at combating desertifi cation. In this study we quantitatively and qualitatively describe several western Mongolian steppe plant communities by examining species composition, plant volume and community structure. Study sites were located in the Uvs and Khovd provinces and had all been affected by livestock grazing. A total of 48 species were found. Stipa krylovii, S. gobica, Cleistogenes songorica, Koeleria cristata and Ajania achilleoides were dominant. There was a signifi cant relationship between biomass and plant volume at all sites. Study sites were classifi ed into four groups using cluster analysis, based on the presence or absence of several species. More than 90% of plant volumes at all groups were perennial grasses and perennial forbs. The ratio of C 3 to C 4 plants at site 3 was reversed in comparison to the other sites. Species highly palatable to livestock were dominant at all sites. To ensure the sustainable use of biological resources in these arid areas, these fi ndings should be taken into account in designing land-use plans.

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Section: Discussionmentioning

confidence: 87%

Community Structure and Productivity in Western Mongolian Steppe

2014

Mong. J. Biol. Sci.

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“…Another protein, copper-transporting p-type ATPase (SCVPRZ2044C07) ( Table 2 ), is expressed in response to oxidative stress and hormone signaling [ 49 ]. The expression of the proteins drought-inducible protein 1os (SCQSST3116H01) ( Table 2 ) [ 50 ] and S-adenosylmethionine:2-demethylmenaquinone methyltransferase-like (SCJFRZ2009B04) ( Table 2 ) is also related to plant adaptation to drought stress [ 51 ]. The expression of these proteins suggests that the cultures are experiencing some type of stress and which may be responsible for the physiological modulation of the cells, thereby allowing the embryogenic competence [ 52 ].…”

Section: Discussionmentioning

confidence: 99%

Label-Free Quantitative Proteomics of Embryogenic and Non-Embryogenic Callus during Sugarcane Somatic Embryogenesis

et al. 2015

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The development of somatic cells in to embryogenic cells occurs in several stages and ends in somatic embryo formation, though most of these biochemical and molecular changes have yet to be elucidated. Somatic embryogenesis coupled with genetic transformation could be a biotechnological tool to improve potential crop yields potential in sugarcane cultivars. The objective of this study was to observe somatic embryo development and to identify differentially expressed proteins in embryogenic (E) and non-embryogenic (NE) callus during maturation treatment. E and NE callus were cultured on maturation culture medium supplemented with different concentrations (0.0, 0.75, 1.5 and 2.0 g L-1) of activated charcoal (AC). Somatic embryo formation and differential protein expression were evaluated at days 0 and 21 using shotgun proteomic analyses. Treatment with 1.5 g L-1 AC resulted in higher somatic embryo maturation rates (158 somatic embryos in 14 days) in E callus but has no effect in NE callus. A total of 752 co-expressed proteins were identified through the SUCEST (The Sugarcane EST Project), including many housekeeping proteins. E callus showed 65 exclusive proteins on day 0, including dehydrogenase, desiccation-related protein, callose synthase 1 and nitric oxide synthase. After 21 days on maturation treatment, 14 exclusive proteins were identified in E callus, including catalase and secreted protein. NE callus showed 23 exclusive proteins on day 0 and 10 exclusive proteins after 21 days on maturation treatment, including many proteins related to protein degradation. The induction of maturation leads to somatic embryo development, which likely depends on the expression of specific proteins throughout the process, as seen in E callus under maturation treatment. On the other hand, some exclusive proteins can also specifically prevent of somatic embryos development, as seen in the NE callus.

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“…The putative C. songorica polyamine and ethylene pathway protein sequences were analyzed according to Pfam family using Hidden Markov Model [23] within Pfam database https://pfam.sanger.ac.uk/search [33]. The resulting C. songorica protein sequences were then submitted to SMART (https://smart.emblheidelberg.de/) [34] and to the conserved domain search within NCBI (https://www.ncbi.nlm.nih.gov/Structure/cdd/wrpsb.cgi) databases [35] to confirm the presence of ethylene and polyamine related pathway gene protein domains.…”

Section: Identification Of C Songorica Polyamine and Ethylene Pathwamentioning

confidence: 99%

“…Cleistogenes songorica, a xerophyte C4 desert plant, is widely distributed in wild areas in the north west part of China where annual precipitations are around 100 mm. It is known to have novel drought stress adaptation strategies and contains rich pools of stress tolerance genes [23]. Earlier studies have shown that transgenic Arabidopsis lines expressing C. songorica LEA genes exhibited improved salt and drought tolerance by reducing CO 2 assimilation levels [24].…”

Section: Introductionmentioning

confidence: 99%

Polyamine and Ethylene pathways crosstalk revisited on a genome wide and gene function scale

Muvunyi¹,

Wu²,

Yan³

et al. 2019

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Background Polyamine and ethylene biosynthesis pathway genes are widely involved in the regulation of plant abiotic stresses. For their biosynthesis, both pathways require the same precursor, Synthase Adenosyl Methionine (SAM) enzyme. Whether they function as competitors or collaborators to regulate plant abiotic stress tolerance is still an elusive topic. Genome wide analysis of Cleistogenes songorica polyamine and ethylene pathway gene families was conducted to study their evolutionary relationship. And, using Arabidopsis plants transformed with a polyamine gene SAMDC2 from C. songorica, the expression of key genes from both pathways, and other previously well-studied stress responsive genes was investigated under salt or drought stress. Further, the ABA’s role on this interaction salt stress was also studied. Results 17 polyamine, 12 ethylene and 6 SAM biosynthesis related genes were identified at genome wide level in C. songorica. Phylogenetic analysis revealed close evolutionary similarities between gene families from both pathways. Also, analysis of cis regulatory elements indicated that SAM family genes promoters were rich into both ABA and ethylene related cis regulatory elements. Transcriptomic analysis, qRT-PCR validation, and confirmation using transgenic Arabidopsis showed that polyamine and ethylene key pathway genes can be concurrently expressed during abiotic stresses. Arabidopsis plants expressing a polyamine gene CsSAMDC2 driven by RD29A showed an improved drought and salt stress tolerance, and an increased expression of key polyamine and ethylene pathway genes. These plants maintained higher chlorophyll content and photosynthetic capacity. Morphological analysis of transgenic seedlings showed that leaves of these lines exhibited a more compact architecture following salt stress exposure. Application of ABA on transgenic lines under salt stress further improved the expression of polyamine and ethylene pathway genes. Further, lateral and primary root development were found improved during salt stress and ABA treatments. Interestingly, the expression of ethylene pathway genes was not reversed by exogenous ABA during salt stress treatment. Conclusion In silico and gene functional analysis assays revealed potential evolutionary and functional similarities between polyamine and ethylene pathway gene families. Such findings imply a synergetic interaction between polyamine and ethylene pathways, and the significant role of ABA on this crosstalk.

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Targeted mining of drought stress-responsive genes from EST resources in Cleistogenes songorica

Cited by 20 publications

References 55 publications

Community Structure and Productivity in Western Mongolian Steppe

Community Structure and Productivity in Western Mongolian Steppe

Label-Free Quantitative Proteomics of Embryogenic and Non-Embryogenic Callus during Sugarcane Somatic Embryogenesis

Polyamine and Ethylene pathways crosstalk revisited on a genome wide and gene function scale

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