Targeted modulation and enhancement of macrophages via sonodynamic therapy-driven cupferroptosis-like stress for implant-associated biofilm infections

Zhang, Xudong; Wang, Zhengxi; Liu, Quan; Hu, Xianli; Mei, Jiawei; Xu, Dongdong; Zhou, Jun; Zhang, Xianzuo; Li, Qianming; Chen, Hua; Su, Zheng; Zhu, Wanbo; Zhu, Chen

doi:10.1016/j.nantod.2023.102092

Cited by 11 publications

(2 citation statements)

References 20 publications

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“…Next, we observed that the PB + NIR and PCZs + NIR groups exhibited a perforation effect on bacterial cell walls, disrupting their integrity, as evidenced by the protein leakage and o -nitrophenyl-β- d -galactopyranoside (ONPG) assay experiments (Figures f,g, S21 and S22). Meanwhile, the eDNA content in the biofilm matrix decreased significantly after the PB + NIR and PCZs + NIR treatments (Figures h and S23). In an acidic biofilm environment, PCZs nanoadjuvants exhibited POD-like activity, which could execute the generation of a significant amount of • OH (Figure i).…”

Section: Results and Discussionmentioning

confidence: 99%

Balancing Bioresponsive Biofilm Eradication and Guided Tissue Repair via Pro-Efferocytosis and Bidirectional Pyroptosis Regulation during Implant Surgery

Wang,

Zhang,

Liu

et al. 2024

ACS Nano

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There is an increasingly growing demand to balance tissue repair guidance and opportunistic infection (OI) inhibition in clinical implant surgery. Herein, we developed a nanoadjuvant for all-stage tissue repair guidance and biofilm-responsive OI eradication via in situ incorporating Cobaltiprotoporphyrin (CoPP) into Prussian blue (PB) to prepare PB-CoPP nanozymes (PCZs). Released CoPP possesses a proefferocytosis effect for eliminating apoptotic and progressing necrotic cells in tissue trauma, thus preventing secondary inflammation. Once OIs occur, PCZs with switchable nanocatalytic capacity can achieve bidirectional pyroptosis regulation. Once reaching the acidic biofilm microenvironment, PCZs possess peroxidase (POD)-like activity that can generate reactive oxygen species (ROS) to eradicate bacterial biofilms, especially when synergized with the photothermal effect. Furthermore, generated ROS can promote macrophage pyroptosis to secrete inflammatory cytokines and antimicrobial proteins for biofilm eradication in vivo. After eradicating the biofilm, PCZs possess catalase (CAT)-like activity in a neutral environment, which can scavenge ROS and inhibit macrophage pyroptosis, thereby improving the inflammatory microenvironment. Briefly, PCZs as nanoadjuvants feature the capability of all-stage tissue repair guidance and biofilm-responsive OI inhibition that can be routinely performed in all implant surgeries, providing a wide range of application prospects and commercial translational value.

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Section: Results and Discussionmentioning

confidence: 99%

Balancing Bioresponsive Biofilm Eradication and Guided Tissue Repair via Pro-Efferocytosis and Bidirectional Pyroptosis Regulation during Implant Surgery

Wang,

Zhang,

Liu

et al. 2024

ACS Nano

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“…1 Implant infection is a common post-operative complication due to the increased risk of bacteria colonization on the solid surface of implants. 2–4 Implant infections not only lead to implant failure but can also result in long-term disabilities or even death for patients. Statistics show that approximately 25% of healthcare costs in the USA are related to implant infections.…”

Section: Introductionmentioning

confidence: 99%

Ultrasound-responsive gallium protoporphyrin and oxygen loaded perfluoropentane nanodroplets for effective sonodynamic therapy of implant infections

Yang,

Chen,

et al. 2024

Nanoscale

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Intrabacterial Nitroreductase‐Activated Imaging and Persistently Illuminated Photodynamic Therapy for Intracellular Infection Theranostics

Ran,

Cao,

Zheng

et al. 2024

Adv Funct Materials

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Intracellular bacterial (ICB) infection is one of the most life‐threatening cases of drug resistance, and photodynamic therapy (PDT) is challenged by limited light penetration into tissues and unspecific toxicity to host cells. Herein, theranostic nanoparticles (NPs) are developed to facilitate on‐demand activation of photosensitizers inside ICBs and persistent PDT of deep‐tissue ICB infections. Mechanoluminescent SrAl2O4:Eu2+ and persistence luminescent CaS:Eu2+ are sequentially deposited on mesoporous silica to prepare mSC, followed by encapsulation by bacterial membranes with loaded nitroreductase (NTR)‐activatable methylene blue (nMB) to obtain mSC‐nMB@BM NPs. The fluorescence quenching of nMB with mSC offers few cytotoxicities even under ultrasonication, and bacterial membrane coatings mediate the specific NP internalization into ICB‐infected macrophages. Bacterial pore‐forming toxins trigger nMB release from NPs, and intrabacterial NTR rejuvenates methylene blue probes from nMB for real‐time imaging of ICB infections. Ultrasonication of NPs continuously excites the rejuvenated methylene blue to generate reactive oxidative species and reduce viable ICBs by 3.54 log magnitude folds. NP treatment on ICB‐infected mice exhibits full survival and restores viscera functions without any pathological abnormality. Therefore, this concise NP design demonstrates capabilities of bacterial membrane‐mediated specific internalization, bacterial toxin‐triggered release and intrabacterial NTR‐rejuvenation of fluorescent probes, and persistent internal light‐illumination for ICB theranostics.

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Targeted modulation and enhancement of macrophages via sonodynamic therapy-driven cupferroptosis-like stress for implant-associated biofilm infections

Cited by 11 publications

References 20 publications

Balancing Bioresponsive Biofilm Eradication and Guided Tissue Repair via Pro-Efferocytosis and Bidirectional Pyroptosis Regulation during Implant Surgery

Balancing Bioresponsive Biofilm Eradication and Guided Tissue Repair via Pro-Efferocytosis and Bidirectional Pyroptosis Regulation during Implant Surgery

Ultrasound-responsive gallium protoporphyrin and oxygen loaded perfluoropentane nanodroplets for effective sonodynamic therapy of implant infections

Intrabacterial Nitroreductase‐Activated Imaging and Persistently Illuminated Photodynamic Therapy for Intracellular Infection Theranostics

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