Targeted nanodiamonds for identification of subcellular protein assemblies in mammalian cells

Lake, Michael P.; Bouchard, Louis‐S.

doi:10.1371/journal.pone.0179295

Cited by 17 publications

(22 citation statements)

References 55 publications

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“…Lake et al utilized nanodiamonds for transmission electron microscopy (TEM) to label protein clusters . They linked the diamonds to antibodies which targeted the nuclear pore complex.…”

Section: Labelingmentioning

confidence: 99%

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Nanodiamonds and Their Applications in Cells

Chipaux

Laan

Hemelaar

et al. 2018

Small

192

195

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Diamonds owe their fame to a unique set of outstanding properties. They combine a high refractive index, hardness, great stability and inertness, and low electrical but high thermal conductivity. Diamond defects have recently attracted a lot of attention. Given this unique list of properties, it is not surprising that diamond nanoparticles are utilized for numerous applications. Due to their hardness, they are routinely used as abrasives. Their small and uniform size qualifies them as attractive carriers for drug delivery. The stable fluorescence of diamond defects allows their use as stable single photon sources or biolabels. The magnetic properties of the defects make them stable spin qubits in quantum information. This property also allows their use as a sensor for temperature, magnetic fields, electric fields, or strain. This Review focuses on applications in cells. Different diamond materials and the special requirements for the respective applications are discussed. Methods to chemically modify the surface of diamonds and the different hurdles one has to overcome when working with cells, such as entering the cells and biocompatibility, are described. Finally, the recent developments and applications in labeling, sensing, drug delivery, theranostics, antibiotics, and tissue engineering are critically discussed.

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“…Lake et al utilized nanodiamonds for transmission electron microscopy (TEM) to label protein clusters . They linked the diamonds to antibodies which targeted the nuclear pore complex.…”

Section: Labelingmentioning

confidence: 99%

“…[116] Lake et al utilized nanodiamonds for transmission electron microscopy (TEM) to label protein clusters. [117] They linked the Small 2018, 14, 1704263 Figure 4. Examples of bioimaging applications of nanodiamonds.…”

Section: Labels For Electron Microscopy Cathodoluminescence and Intmentioning

confidence: 99%

Nanodiamonds and Their Applications in Cells

Chipaux

Laan

Hemelaar

et al. 2018

Small

192

195

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“…FND particles are also clearly visible with electron microscopy 42 and can be identified by very high electron density, localized within a small volume, and high local con-trast at the edge of the particle. 43 These properties make them distinct from other intracellular components (ESI Fig. 6 †).…”

Section: Trypsin Treatment Yields Higher Proportion Of Cells With Intmentioning

confidence: 99%

Nanodiamond uptake in colon cancer cells: the influence of direction and trypsin-EDTA treatment

et al. 2019

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Nanoparticles are routinely used in cell biology. They deliver drugs or function as labels or sensors. For many of these applications it is essential that the nanoparticles enter the cells. While some cell types readily ingest all kinds of particles, others just don't. We report that uptake can be enhanced for some cells if the particles are administered from the basolateral side of the cells (in this case from below).Compared to apical uptake (from above), we report an 8-fold increase in the number of fluorescent nanodiamonds internalized by the colon cancer cell line HT29. Up to 96% of the cells treated by a modified protocol contain at least one nanodiamond, whereas in the control group we could observe nanodiamonds in less than half of the cells. We were also able to show that simple treatment of cell clusters with trypsin-EDTA leads to the same enhancement of the nanodiamond uptake as seeding the cells on top of the nanoparticles. Although our study is focused on nanodiamonds in HT29 cells, we believe that this method could also be applicable for other nanoparticles and cells with a specific directionality. † Electronic supplementary information (ESI) available. See

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“…The scientists describe an application for diamond nanoparticles as targeted intensity contrast labels in biological TEM using the nuclear pore complex (NPC) as a model macroassembly which is possible as a way of delivery of antibody-conjugated (Nup-98 nucleoporin) nanodiamonds to live mammalian cells using maltotriose-conjugated polypropylenimine dendrimers. As a result, the efficient localization of nanodiamonds to the intended cellular target is possible (101).…”

Section: Nanodiamonds As Fluorescent Labelsmentioning

confidence: 99%

Possibilities of nanodiamonds application – biological and medical aspects

Solarska-Ściuk¹,

Kleszczyńska²

2019

Acta Poloniae Pharmaceutica - Drug Research

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This topical review briefly discusses selected highlights of research on diamond nanoparticles obtained by different methods and their potential applications in biology and medicine. In recent years nanotechnology has aroused interest of a large number of scientists who endeavor to obtain nanoparticles (which differ in size and structure of surface) using different methods, in order to determine their physical, chemical and biological properties that are in relation to the methods used in the process of their production. The knowledge developed in this way will be beneficial in an attempt to use nanoparticles more reasonably in various branches of science. The distinguishing features of carbon nanoparticles are their biocompatibility, photostability and easily chemically modified surface that results in high usefulness for intracellular studies. What is more, low toxicity of nanoparticles with many cell lines is at the center of scientific interest. This, in turn, leads to a large number of biomedical applications. The property that nanodiamonds are able to penetrate cells through endocytosis, allows delivering the drug connected with nanoparticles into cancer cells. These features of nanoparticles have given many promising leads in nanooncology, in particular in drug delivery, diagnosis, imaging, and therapy. This paper presents a summary of different classes of nanodiamond particles, the ways of their uptake into cells, an overview of the possible application of nanoparticles as nanocarriers and as a clinical theranostic platform, as well as advantages and disadvantages of using nanodiamonds in biomedicine.

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Targeted nanodiamonds for identification of subcellular protein assemblies in mammalian cells

Cited by 17 publications

References 55 publications

Nanodiamonds and Their Applications in Cells

Nanodiamonds and Their Applications in Cells

Nanodiamond uptake in colon cancer cells: the influence of direction and trypsin-EDTA treatment

Possibilities of nanodiamonds application – biological and medical aspects

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