Targeted NIR-responsive theranostic immuno-nanomedicine combined TLR7 agonist with immune checkpoint blockade for effective cancer photothermal immunotherapy

Yasothamani, Vellingiri; Vivek, Raju

doi:10.1039/d2tb01195f

Cited by 17 publications

(14 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Both ATP and HMGB1 release levels were raised in tumor cells treated with NC and 808 nm NIR irradiation compared to non-anti-PD-L1 groups. PTT-induced ICD causes dying tumor cells to release DAMPs, which can activate APCs and boost tumor-specific T lymphocyte production . TNF-α and IL-6 are two immune-related cytokines produced by DCs that are tested and considered to be markers of DC activity.…”

Section: Results and Discussionmentioning

confidence: 99%

“…PTT-induced ICD causes dying tumor cells to release DAMPs, which can activate APCs and boost tumor-specific T lymphocyte production. 52 TNF-α and IL-6 are two immune-related cytokines produced by DCs that are tested and considered to be markers of DC activity. The populations of matured DCs for the NC with anti-PD-L1 injection and 808 NIR nm laser irradiation-treated group increased by 55% compared to those of the NC group without anti-PD-L1 (Figure 8e).…”

Section: Nc-mediated Photothermal Activation Of Icd-based Immune Resp...mentioning

confidence: 99%

See 1 more Smart Citation

Near-Infrared Light-Activated Dual Targeting with Peptide-Conjugated Mesoporous Silica Nanoparticles for Multimodal Anticancer Therapy

Gowsalya

Karthikeyan

Vivek

2022

ACS Appl. Nano Mater.

Self Cite

View full text Add to dashboard Cite

The nuclear-targeted delivery system (NTDS) is one of the major advancements in drug delivery. It acts efficiently by specifically destroying cancer cells. On mesoporous silica nanoparticles (MSNs), a trans-activator of transcription peptide was conjugated. A targeted multifunctional NTDS is a novel form of nanotherapeutics, where the drug is selectively targeted to specific cell nuclei. Herein, we have successfully fabricated a cervical cancer receptor and nuclear targeting formulation of MSNs integrated with paclitaxel (PTX) and indocyanine green (ICG), thus forming a novel composite denoted as NC. To successfully achieve NTDS, we have introduced an effective strategy for constructing a nuclear-targeted MSNs, which possesses an extraordinary photothermal effect to ablate the cancer cells and simultaneously inhibit recurrence in the tumor microenvironment. The NC rapidly increases the temperature of the nucleus and destroys genetic substances of cancer cells when exposed to an 808 nm near-infrared laser. Following that, PDT selectively destroys tumor cells by creating singlet oxygen species, which is paired with effective PTX-based treatment. Photothermally ablated tumor cells can induce the immunogenic cell death (ICD) of tumor cells with the release of tumor-associated antigens and damage-associated molecular patterns (DAMPs). ICD markers include the release of HMGB1, ATP, and CRT. To address the restrictions of NC, an anti-PD-L1-activated nanosystem was developed to potentiate the antitumor efficacy along with photo/chemo/immunotherapy. Therefore, multimodal combinatorial therapy will be more effective on tumors and will reduce tumor recurrence. This work provides vital guidance for MSNs-based PTT/ PDT/chemo/immunotherapy applications in cancer therapy.

show abstract

Section: Results and Discussionmentioning

confidence: 99%

Section: Nc-mediated Photothermal Activation Of Icd-based Immune Resp...mentioning

confidence: 99%

Near-Infrared Light-Activated Dual Targeting with Peptide-Conjugated Mesoporous Silica Nanoparticles for Multimodal Anticancer Therapy

Gowsalya

Karthikeyan

Vivek

2022

ACS Appl. Nano Mater.

Self Cite

View full text Add to dashboard Cite

show abstract

“…ICD markers of various DAMPs includes the cell surface exposure of CRT, ATP, and HMGB1 (Figure 5 d to g) . In this work, we noticed the tumor cell death connected to ICD markers of the DAMPs exposed during photothermal ablation of tumor, and the mechanism by which they activate the immune system 53 . Accordingly, the previous work also supported that the HCuSNPs-CpG NPs for cancer photothermal immunotherapy in a mouse model 30 .…”

Section: Discussionmentioning

confidence: 89%

Ultra-small NIR-Responsive Nanotheranostic Agent for Targeted Photothermal Ablation Induced Damage-Associated Molecular Patterns (DAMPs) from Post-PTT of Tumor Cells Activate Immunogenic Cell Death

Sobhana¹,

Sarathy²,

Karthikeyan³

et al. 2023

Nanotheranostics

Self Cite

View full text Add to dashboard Cite

Theranostic nanoparticles (TNPs) is an efficient avenue that culminates both diagnosis and therapy into cancer treatment. Herein, we have formulated a theranostic nanocomposite (NC) with CuS being the ultra-small core component. To ensure stability to the NC, PEI was added which is a vital anchoring group polymer, especially on sulfide surfaces, and adds quality by being a better stabilizer and reducing agent. Additionally, to add stability, specificity, and added photothermal efficiency to the fabricated NC. In addition, encapsulation of indocyanine green (ICG), an efficient NIR absorber, and Folic acid (FA) were conjugated systematically, characterized, and analyzed for photo-stability. The photothermal conversion efficiency of the novel NC (CuS-PEI-ICG-FA) was analyzed at 808 nm, where the NC efficiently converted light energy to heat energy. The NC was also tested for hemocompatibility to clarify and also determined biocompatibility. Surprisingly, damage-associated molecular patterns (DAMPs) from post-PTT of tumor cells activate immunogenic cell death (ICD) for tumor-specific immune responses. The deserving photothermal performance and photo-stability makes the NC an ideal platform for photoacoustic imaging (PAI). A superior contrast was observed for PAI in a concentration-dependent manner enhancing the level of penetration into tissues, thereby better imaging. On account of this study, the newly formulated NC could be utilized as a "nanotheranostic" designed for therapeutic and image diagnostic agent of cancer biomedical applications.

show abstract

“…5−8 NIR-induced PTT (NIR-I, 650−950 nm) has shown outstanding results in both research and clinical trials over the last few years. In comparison with the NIR-I optical window, the NIR-II optical window (1000−1350 nm) offers greater tissue penetration, lower photon absorption and scattering, and maximum permitted exposure (MPE) (1 W/ cm 2 ), which makes it more efficient and clinically applicable. The MPE for tissue exposure is 1 W/cm 2 in the NIR-II region, whereas it is 0.33 W/cm 2 at 808 nm in the NIR-I region.…”

Section: ■ Introductionmentioning

confidence: 99%

TLR-7/8 Agonist-Loaded Polypyrrole-Based Theranostic Nanovaccine for Second Near-Infrared Photothermal Immunotherapy of Cancer

Karthikeyan

Yasothamani

Haldorai

et al. 2023

ACS Appl. Nano Mater.

Self Cite

View full text Add to dashboard Cite

Functionalized theranostic agents have the potential to be an effective tool for targeting tumor cells with an effective response in cancer imaging and therapy. Particularly, near-infrared (NIR) light is extremely advantageous and can penetrate deeply into biological tissues with minimum attenuation and photodamage to cells. The major impediment to the translation of research into clinical applications is toxicity and long-term body retention. Designing renal clearable and theranostic qualities in a single nanoplatform remains the biggest challenge. Especially, nanomaterials have optical absorption in the second biowindow (NIR-II) with deep penetration and less tissue scattering. Photothermal therapy (PTT), which uses hyperthermia produced by photothermal agents (PTAs) that convert light irradiation to ablate tumors, has received great attention due to its noninvasiveness. Herein, we developed an NIR-II-responsive ultrasmall polypyrrole (PPy)-based theranostic agent conjugated with TAT peptide that has a unique nuclear-targeting property that allows for efficient nuclear-targeted photothermal ablation and immunogenic cell death (ICD). Furthermore, PPy decorated with hyaluronic acid (HA)-loaded toll-like receptor 7/8 immune-activating drug resiquimod (R848), a synthetic agonist for the maturation of dendritic cells. Therefore, the prepared nanocomposite leads to effective tumor cell nuclear ablation, together with a photothermal immune response and renal clearance. This strategy may provide another avenue for highly efficient cancer theranostic nanovaccines.

show abstract

Targeted NIR-responsive theranostic immuno-nanomedicine combined TLR7 agonist with immune checkpoint blockade for effective cancer photothermal immunotherapy

Abstract: Nanomedicine with immunotherapy will offer the opportunities to target cancer in an effective manner; however, remains challenging. We herein report a photothermal material loaded with immune-adjuvant combined immune checkpoint blockade...

Cited by 17 publications

References 42 publications

Near-Infrared Light-Activated Dual Targeting with Peptide-Conjugated Mesoporous Silica Nanoparticles for Multimodal Anticancer Therapy

Near-Infrared Light-Activated Dual Targeting with Peptide-Conjugated Mesoporous Silica Nanoparticles for Multimodal Anticancer Therapy

Ultra-small NIR-Responsive Nanotheranostic Agent for Targeted Photothermal Ablation Induced Damage-Associated Molecular Patterns (DAMPs) from Post-PTT of Tumor Cells Activate Immunogenic Cell Death

TLR-7/8 Agonist-Loaded Polypyrrole-Based Theranostic Nanovaccine for Second Near-Infrared Photothermal Immunotherapy of Cancer

Contact Info

Product

Resources

About