Targeted photodynamic therapy of breast cancer cells using antibody-phthalocyanine-gold nanoparticle conjugates

Stuchinskaya, Tanya; Moreno, Miguel; Cook, Michael J.; Edwards, Dylan R.; Russell, David A.

doi:10.1039/c1pp05014a

Cited by 308 publications

(233 citation statements)

References 39 publications

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“…Au has also been studied on its effects on breast cancer cells. Stuchinskaya et al, conjugated the Au nanoparticles with a phthalocyanine-antibody complex which proved to be a promising way of targeting and killing breast cancer cells by the Au nanoparticles and cytotoxic singlet oxygen producer phthalocyanine [77].…”

Section: Applications Of Metallic Nanoparticlesmentioning

confidence: 99%

Biological Synthesis of Metallic Nanoparticles by Bacteria, Fungi and Plants

Pantidos¹

2014

J Nanomed Nanotechnol

484

221

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Over the past few decades interest in metallic nanoparticles and their synthesis has greatly increased. This has resulted in the development of numerous ways of producing metallic nanoparticles using chemical and physical methods. However, drawbacks such as the involvement of toxic chemicals and the high-energy requirements of production make it difficult for them to be widely implemented. An alternative way of synthesising metallic nanoparticles is by using living organisms such as bacteria, fungi and plants. This "green" method of biological nanoparticle production is a promising approach that allows synthesis in aqueous conditions, with low energy requirements and low-costs. This review gives an overview of some of these environmentally friendly methods of biological metallic nanoparticle synthesis. It also highlights the potential importance of these methods in assessing nanoparticle risk to both health and the environment.techniques, which are generally low-cost and high-volume. However, the need for toxic solvents and the contamination from chemicals used in nanoparticle production limit their potential use in biomedical applications [15]. Therefore a "green", non-toxic way of synthesising metallic nanoparticles is needed in order to allow them to be used in a wider range of industries. This could potentially be achieved by using biological methods.Many bacteria, fungi and plants have shown the ability to synthesise metallic nanoparticles and all have their own advantages and disadvantages (Table 1) [16][17][18]. Intracellular or extracellular synthesis, growth temperature, synthesis time, ease of extraction and percentage synthesised versus percentage removed from sample ratio, all play an important role in biological nanoparticle production. Finding the right biological method can depend upon a number of variables. Most importantly, the type of metal nanoparticle under investigation is of vital consideration, as in general organisms have developed resistance against a small number of metals, potentially limiting the choice of organism. However synthetic biology; a nascent field of science, is starting to address these issues in order to create more generalised chassis, able to synthesise more than one type of metallic nanoparticle using the same organism [19]."Natural" biogenic metallic nanoparticle synthesis can be split into two categories. The first is bioreduction, in which metal ions are chemically reduced into more stable forms biologically. Many organisms have the ability to utilise dissimilatory metal reduction, in which the reduction of a metal ion is coupled with the oxidation of an enzyme [20]. This results in stable and inert metallic nanoparticles that can then be safely removed from a contaminated sample. The second category is biosorption. This involves the binding of metal ions from Journal of Nanomedicine & Nanotechnology J o u rna l of N a n o m ed icine & N a n o te chnolo g y

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Section: Applications Of Metallic Nanoparticlesmentioning

confidence: 99%

Biological Synthesis of Metallic Nanoparticles by Bacteria, Fungi and Plants

Pantidos¹

2014

J Nanomed Nanotechnol

484

221

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“…21 These authors have shown that the nanoparticle conjugates with the anti-erbB2 antibody selectively target the erbB2 receptor that is overexpressed on SK-BR-3 breast cancer cells while having limited or no targeted photoactivity for the MDA-MB-231 breast cancer cells (breast cancer cell line without erbB2 amplification) or normal mammary epithelial cells (MCF-10A). In addition, targeting of the T antigen on the surface of human colorectal adenocarcinoma cells has been achieved using the lectin Jacalin conjugated to phthalocyanine functionalised gold nanoparticles.…”

Section: Introductionmentioning

confidence: 99%

Water soluble, multifunctional antibody-porphyrin gold nanoparticles for targeted photodynamic therapy

Penon

Marín

Russell

et al. 2017

Journal of Colloid and Interface Science

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Photodynamic therapy (PDT) is a treatment of cancer by which tumour cells are destroyed using reactive oxygen species produced by photosensitisers following activation with visible or near infrared light. Successful PDT depends on the solubility and the targeting ability of the photosensitisers. In this work, the synthesis of a porphyrin-based water soluble nanoparticle conjugate containing a targeting agent that recognises the erbB2 receptor overexpressed on the surface of particular cancer cells is reported. The nanoparticle conjugates were synthesised following two different protocols, viz. a biphasic and a monophasic method, with the aim to determine which method yielded the optimal nanosystem for potential PDT applications. The nanoparticles were characterised using UV-Vis absorption and fluorescence spectroscopies together with transmission electron microscopy and zeta potential measurements; and their ability to produce singlet oxygen following irradiation was investigated following the decay in absorption of a singlet oxygen probe. The nanoparticles synthesised using the monophasic method were shown to produce the highest amount of singlet oxygen and were further functionalised with anti-erbB2 antibody to target the erbB2 receptors expressed on the surface of SK-BR-3 human breast cancer cells. The water soluble, antibody-porphyrin nanoparticle conjugates were shown to elicit targeted PDT of the breast cancer cells.

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“…The HEPES and PBS 26 The jacalin or anti-HER-2 antibody conjugated C11Pc-PEG gold nanoparticles prepared for use with the SK-BR-3 cells were redispersed in serum-free Dulbecco's Modified Eagle Medium (DMEM; Invitrogen) supplemented with 1 mM L-Gln, as previously described. 11 As a control, non-conjugated C11Pc-PEG gold nanoparticles were also prepared in serum-free RPMI 1640 medium or serum-free DMEM medium, depending on the cell line used. All nanoparticle preparations were characterised using a Hitachi U-3010 UV-visible absorption spectrophotometer, appropriately diluted for in vitro experiments using either serum-free RPMI 1640 medium or serum-free DMEM medium, and stored at 4°C.…”

Section: Synthesis Of the C11pc-peg Gold Nanoparticles And Conjugatiomentioning

confidence: 99%

Cancer targeting with biomolecules: a comparative study of photodynamic therapy efficacy using antibody or lectin conjugated phthalocyanine-PEG gold nanoparticles

Obaid

Chambrier

Cook

et al. 2015

Photochem Photobiol Sci

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125

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The functionalisation of therapeutic nanoparticle constructs with cancer-specific biomolecules can enable selective tumour accumulation and targeted treatment. Water soluble gold nanoparticles (ca. 4 nm) stabilised by a mixed monolayer of a hydrophobic zinc phthalocyanine photosensitiser (C11Pc) and hydrophilic polyethylene glycol (PEG) have been prepared. The C11Pc-PEG gold nanoparticle constructs were further functionalised with jacalin, a lectin specific for the cancer-associated Thomsen-Friedenreich (T) carbohydrate antigen, or with monoclonal antibodies specific for the human epidermal growth factor receptor-2 (HER-2). The two biofunctionalised nanoparticle conjugates produced similar levels of singlet oxygen upon irradiation at 633 nm. Importantly, both nanoparticle conjugates demonstrated extensive, yet comparable, phototoxicity in HT-29 colorectal adenocarcinoma cells (80-90%) and in SK-BR-3 breast adenocarcinoma cells (>99%). Non-conjugated C11Pc-PEG gold nanoparticles were only minimally phototoxic. Lysosomal colocalisation studies performed with the HT-29 colon cancer cells and the SK-BR-3 breast cancer cells revealed that both nanoparticle conjugates were partially localised within acidic organelles, which is typical of receptor-mediated endocytosis. The similarity of the targeted PDT efficacy of the two biofunctionalised C11Pc-PEG gold nanoparticles is discussed with respect to targeting ligand binding affinity and cell surface antigen density as key determinants of targeting efficiency. This study highlights how targeting small cell-surface molecules, such as the T antigen, can mediate a selective photodynamic treatment response 2 which is similar to that achieved when targeting overexpressed protein receptors, such as HER-2. The high prevalence of the T antigen present on the cellular surface of primary tumours emphasises the broad potential applications for lectin-targeted therapies.

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Targeted photodynamic therapy of breast cancer cells using antibody-phthalocyanine-gold nanoparticle conjugates

Abstract: This article is published as part of a themed issue of Photochemical & Photobiological Sciences on Drug delivery technologies and immunological aspects of photodynamic therapy

Cited by 308 publications

References 39 publications

Biological Synthesis of Metallic Nanoparticles by Bacteria, Fungi and Plants

Biological Synthesis of Metallic Nanoparticles by Bacteria, Fungi and Plants

Water soluble, multifunctional antibody-porphyrin gold nanoparticles for targeted photodynamic therapy

Cancer targeting with biomolecules: a comparative study of photodynamic therapy efficacy using antibody or lectin conjugated phthalocyanine-PEG gold nanoparticles

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