Targeted preparation and recognition mechanism of broad-spectrum antibody specific to Aconitum alkaloids based on molecular modeling and its application in immunoassay

Ren, Zhenhui; Zhang, Huixia; Liu, Yang; Wang, Zile; Xiong, Jincheng; Zheng, Pimiao; Wang, Jianyi; Jiang, Haiyang

doi:10.1016/j.aca.2022.340011

Cited by 3 publications

(3 citation statements)

References 47 publications

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“…Crucially, alkaloids, widespread in nature ( 9 ), play a key role in Traditional Chinese Medicine’s therapeutic effects, exhibiting significant anti-cancer, anti-inflammatory, and antioxidant activities ( 10 – 12 ). In this context, Aconitum, commonly known as “wutou,” stands out as a repository of approximately 450 alkaloids and has been extensively utilized for treating various diseases in China, Japan, and other regions ( 13 , 14 ). Aconitum has been traditionally employed to alleviate a spectrum of ailments, including inflammatory conditions, cardiovascular disorders, and nervous system disturbances ( 15 ).…”

Section: Introductionmentioning

confidence: 99%

Songorine modulates macrophage polarization and metabolic reprogramming to alleviate inflammation in osteoarthritis

He,

Huang,

et al. 2024

Front. Immunol.

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IntroductionOsteoarthritis (OA) is a prevalent joint disorder characterized by multifaceted pathogenesis, with macrophage dysregulation playing a critical role in perpetuating inflammation and joint degeneration. MethodsThis study focuses on Songorine, derived from Aconitum soongaricum Stapf, aiming to unravel its therapeutic mechanisms in OA. Comprehensive analyses, including PCR, Western blot, and immunofluorescence, were employed to evaluate Songorine's impact on the joint microenvironment and macrophage polarization. RNA-seq analysis was conducted to unravel its anti-inflammatory mechanisms in macrophages. Metabolic alterations were explored through extracellular acidification rate monitoring, molecular docking simulations, and PCR assays. Oxygen consumption rate measurements were used to assess mitochondrial oxidative phosphorylation, and Songorine's influence on macrophage oxidative stress was evaluated through gene expression and ROS assays.ResultsSongorine effectively shifted macrophage polarization from a pro-inflammatory M1 phenotype to an anti-inflammatory M2 phenotype. Notably, Songorine induced metabolic reprogramming, inhibiting glycolysis and promoting mitochondrial oxidative phosphorylation. This metabolic shift correlated with a reduction in macrophage oxidative stress, highlighting Songorine's potential as an oxidative stress inhibitor.DiscussionIn an in vivo rat model of OA, Songorine exhibited protective effects against cartilage damage and synovial inflammation, emphasizing its therapeutic potential. This comprehensive study elucidates Songorine's multifaceted impact on macrophage modulation, metabolic reprogramming, and the inflammatory microenvironment, providing a theoretical foundation for its therapeutic potential in OA.

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Section: Introductionmentioning

confidence: 99%

Songorine modulates macrophage polarization and metabolic reprogramming to alleviate inflammation in osteoarthritis

He,

Huang,

et al. 2024

Front. Immunol.

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“…3,4 However, due to the low recognition sensitivity of biological antibodies, traditional immune antigen analysis methods were difficult to meet the detection requirements for multiple types and low loads of exposed viruses in the aqueous environment. 5,6 Similarly, polymerase chain reaction (PCR)-based nucleic acid detection methods were equally difficult to satisfy the demand for high-throughput detection of multiplex exposed virus due to their low detection throughput. 7,8 Although multiplex PCR techniques have been developed for the simultaneous detection of multiplex nucleic acid sequences, the competition and interference between primer pairs make them highly susceptible to cross-interference when dealing with complex environmental media.…”

mentioning

confidence: 99%

“…Water-borne viruses pose a serious threat to public health and safety. , Among them, pathogens such as Norovirus and Rotavirus are well-known causative factors that can lead to nonbacterial gastroenteritis in humans. , The World Health Organization has estimated that millions of people worldwide die every year due to infections caused by Norovirus or Rotavirus. , However, due to the low recognition sensitivity of biological antibodies, traditional immune antigen analysis methods were difficult to meet the detection requirements for multiple types and low loads of exposed viruses in the aqueous environment. , Similarly, polymerase chain reaction (PCR)-based nucleic acid detection methods were equally difficult to satisfy the demand for high-throughput detection of multiplex exposed virus due to their low detection throughput. , Although multiplex PCR techniques have been developed for the simultaneous detection of multiplex nucleic acid sequences, the competition and interference between primer pairs make them highly susceptible to cross-interference when dealing with complex environmental media. , In addition, the high technical requirements of PCR methods, and the complex and time-consuming amplification process make it difficult to meet the demand for immediate detection in the field of environmental analysis . Therefore, there are severe barriers to the application of traditional antigen analysis and nucleic acid detection methods for the detection of viruses exposed to the aqueous environment.…”

mentioning

confidence: 99%

Distance-Regulated Photoelectrochemical Sensor “Signal-On” and “Signal-Off” Transitions for the Multiplexed Detection of Viruses Exposed in the Aquatic Environment

Li,

Wang

et al. 2023

Anal. Chem.

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Photochemical (PEC) sensors were severely limited for multiplex detection applications due to the cross interference between multiplex signals at the single recognition interface. In this work, a distance-regulated PEC sensor was developed for multiplex detection by using an i-Motif sequence with conformational transformation activity as the signal transduction unit. Through dynamic regulation of the spatial distance between the end site of the functional sequence and the electrode material, the photogenerated electrons on the surface of the sensor were directionally transferred. Thus, a PEC sensor with "signal-on" and "signal-off" dual signal output modes was developed for simultaneous detection of multitarget molecules. Combining isothermal nucleic acid amplification, the PEC sensor constructed in this work was successfully applied to the detection of two virus (Norovirus and Rotavirus) nucleic acid sequences. Under the optimal condition, this bioassay protocol exhibits a linear range of 0.01−100 nM for both viruses with detection limits of 0.72 and 0.53 pM, respectively. In this study, a stimulus-mediated distance regulation strategy successfully addressed the transduction of multiplex detection signals at the single recognition interface of the PEC sensor. It is expected that the technical barriers to multiplex detection of PEC sensors will be overcome and the application of PEC sensing technology will be expanded in the field of environmental analysis.

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Enantioselectivity regulation of antibody against chiral herbicide metolachlor based on interaction at chiral center

Yu,

Zhong,

Zhao

et al. 2024

International Journal of Biological Macromolecules

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Targeted preparation and recognition mechanism of broad-spectrum antibody specific to Aconitum alkaloids based on molecular modeling and its application in immunoassay

Cited by 3 publications

References 47 publications

Songorine modulates macrophage polarization and metabolic reprogramming to alleviate inflammation in osteoarthritis

Songorine modulates macrophage polarization and metabolic reprogramming to alleviate inflammation in osteoarthritis

Distance-Regulated Photoelectrochemical Sensor “Signal-On” and “Signal-Off” Transitions for the Multiplexed Detection of Viruses Exposed in the Aquatic Environment

Enantioselectivity regulation of antibody against chiral herbicide metolachlor based on interaction at chiral center

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