Targeted quantitative analysis of monoterpenoid indole alkaloids in Alstonia scholaris by ultra‐high‐performance liquid chromatography coupled with quadrupole time of flight mass spectrometry

Abstract

Monoterpene indole alkaloids exhibit structural diversity in herbal resources and have been developed as promising drugs owing to their significant biological activities. Confidential identification and quantification of monoterpene indole alkaloids is the key to quality control of target plants in industrial production but has rarely been reported. In this study, quantitative performance of three data acquisition modes of ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry including full scan, auto-MS 2 and target-MS 2 , was evaluated and compared for specificity, sensitivity, linearity, precision, accuracy, and matrix effect using five monoterpene indole alkaloids (scholaricine, 19-epi-scholaricine, vallesamine, picrinine, and picralinal). Method validations indicated that target-MS 2 mode showed predominant performance for simultaneous annotation and quantification of analytes, and was then applied to determine monoterpene indole alkaloids in Alstonia scholaris (leaves, barks) after extraction procedures optimization using Box-Behnken design of response surface methodology. The variations of A. scholaris monoterpene indole alkaloids in different plant parts, harvest periods, and post-handling processes, were subsequently investigated. The results indicated that target-MS 2 mode could improve the quantitative capability of ultra-high-performance liquid

High‐performance liquid chromatography analysis of alkaloids in various parts of lotus extracts with ion mobility spectrometry and mass spectrometry dual detection

“…The commonly used analytical methods for alkaloids include gas chromatography-mass spectrometry, acid dye colorimetry, UV spectrophotometry, high-performance liquid chromatography (HPLC), and HPLC-mass spectrometry [18][19][20][21][22]. HPLC has the characteristics of simplicity, rapidity, and good reproducibility [23].…”