Targeted Quantitative Mass Spectrometry Analysis of Protein Biomarkers From Previously Stained Single Formalin-Fixed Paraffin-Embedded Tissue Sections

Ackermann, Bradley L.; Morrison, Ryan D.; Hill, Salisha; Westfall, Matthew D.; Butts, Brent D.; Soper, Michael D; Fill, Jeff A.; Schade, Andrew E.; Liebler, D.C.; Gruver, Aaron M.

doi:10.1016/j.labinv.2022.100052

Laboratory Investigation

2023

DOI: 10.1016/j.labinv.2022.100052

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Targeted Quantitative Mass Spectrometry Analysis of Protein Biomarkers From Previously Stained Single Formalin-Fixed Paraffin-Embedded Tissue Sections

Bradley L. Ackermann¹,

Ryan D. Morrison²,

Salisha Hill³

et al.

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2024

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Cited by 3 publications

References 18 publications

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2023 White Paper on Recent Issues in Bioanalysis: Deuterated Drugs; LNP; Tumor/FFPE Biopsy; Targeted Proteomics; Small Molecule Covalent Inhibitors; Chiral Bioanalysis; Remote Regulatory Assessments; Sample Reconciliation/Chain of Custody (PART 1A – Recommendations on Mass Spectrometry, Chromatography, Sample Preparation Latest Developments, Challenges, and Solutions and BMV/Regulated Bioanalysis PART 1B - Regulatory Agencies' Inputs on Regulate

Baratta,

Jian,

Hengel

et al. 2024

Bioanalysis

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2023 White Paper on Recent Issues in Bioanalysis: Deuterated Drugs; LNP; Tumor/FFPE Biopsy; Targeted Proteomics; Small Molecule Covalent Inhibitors; Chiral Bioanalysis; Remote Regulatory Assessments; Sample Reconciliation/Chain of Custody (PART 1A – Recommendations on Mass Spectrometry, Chromatography, Sample Preparation Latest Developments, Challenges, and Solutions and BMV/Regulated Bioanalysis PART 1B - Regulatory Agencies' Inputs on Regulate

Baratta,

Jian,

Hengel

et al. 2024

Bioanalysis

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Optical sensor for fast and accurate lung cancer detection with tissue autofluorescence and diffuse reflectance spectroscopy

Yang,

Chen,

et al. 2024

Thoracic Cancer

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BackgroundCancer is a severe threat to human health, and surgery is a major method of cancer treatment. This study aimed to develop an optical sensor for fast cancer tissue.MethodsThe tissue autofluorescence spectrum and diffuse reflectance spectrum were obtained by using a laboratory‐developed optical sensor system. A total of 151 lung tissue samples were used in this ex vivo study.ResultsExperimental results demonstrate that tissue autofluorescence spectroscopy with a 365‐nm excitation has better performance than diffuse reflectance spectroscopy, and 63 of 64 test samples (98.4% accuracy) were correctly classified with tissue autofluorescence spectroscopy and our developed data analysis method.ConclusionsOur promising ex vivo study results show that the developed optical sensor system has great promise for future clinical translation for intraoperative lung cancer detection and other applications.

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LC–MS Bioanalysis of Protein Biomarkers and Protein Therapeutics in Formalin-Fixed Paraffin-Embedded Tissue Specimens

G Jagadeeshaprasad,

Zeng,

Zheng

2024

Bioanalysis

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Formalin-fixed paraffin-embedded (FFPE) is a form of preservation and preparation for biopsy specimens. FFPE tissue specimens are readily available as part of oncology studies because they are often collected for disease diagnosis or confirmation. FFPE tissue specimens could be extremely useful for retrospective studies on protein biomarkers because the samples preserved in FFPE blocks could be stable for decades. However, LC–MS bioanalysis of FFPE tissues poses significant challenges. In this Perspective, we review the benefits and recent developments in LC–MS approach for targeted protein biomarker and protein therapeutic analysis using FFPE tissues and their clinical and translational applications. We believe that LC–MS bioanalysis of protein biomarkers in FFPE tissue specimens represents a great potential for its clinical applications.

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Targeted Quantitative Mass Spectrometry Analysis of Protein Biomarkers From Previously Stained Single Formalin-Fixed Paraffin-Embedded Tissue Sections

Cited by 3 publications

References 18 publications

Optical sensor for fast and accurate lung cancer detection with tissue autofluorescence and diffuse reflectance spectroscopy

LC–MS Bioanalysis of Protein Biomarkers and Protein Therapeutics in Formalin-Fixed Paraffin-Embedded Tissue Specimens

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