Targeted <scp>RNA</scp> sequencing reveals differential patterns of transcript expression in geographically discrete, insecticide resistant populations of <scp><i>Leptinotarsa decemlineata</i></scp>

Clements, Justin; Lamour, Kurt; Frost, Kenneth E.; Dwyer, J. D.; Huseth, Anders S.; Groves, Russell L.

doi:10.1002/ps.6393

Cited by 2 publications

(2 citation statements)

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“…Much information is also available regarding the molecular leads associated with response to insecticides in L. decemlineata . Modulation of targets involved in xenobiotic metabolism has been reported in populations that were resistant or sensitive to imidacloprid or chlorothalonil ( Clements et al 2021 ). Differential expression of transcripts coding for cytochrome P450 (CYP450) genes in a similar context and RNAi-based approaches to evaluate the impact of varying expression of CYP450 targets on insecticide resistance in L. decemlineata have also been investigated ( Clements et al 2017 , Kalsi and Palli 2017 , Kaplanoglu et al 2017 ).…”

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confidence: 99%

Exposure to Temperature and Insecticides Modulates the Expression of Small Noncoding RNA-Associated Transcripts in the Colorado Potato Beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae)

Youssef

Ouédraogo

Bastarache

et al. 2022

Journal of Insect Science

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The Colorado potato beetle (Leptinotarsa decemlineata (Say)) is an insect that can adapt to various challenges, including temperature fluctuations or select insecticide treatments. This pest is also an ongoing threat to the potato industry. Small noncoding RNAs such as miRNAs, which can control posttranscriptionally the expression of various genes, and piRNAs, which can notably impact mRNA turnover, are modulated in insects under different conditions. Unfortunately, information regarding the expression status of key players involved in their synthesis and function is for the most part lacking. The current study thus aims at assessing the levels of such targets in L. decemlineata exposed to hot and cold temperatures as well as treated to the insecticides chlorantraniliprole, clothianidin, imidacloprid, and spinosad. Transcript expression levels of Ago1, Ago2, Ago3, Dcr2a, Dcr2b, Expo-5, Siwi-1, and Siwi-2, components of pathways associated with small noncoding RNA production or function, were measured by qRT-PCR and revealed modulation of select transcripts in response to temperature challenges and to select insecticides. RNAi-mediated reduction of Ago2 transcript levels in L. decemlineata injected with Ago2-targeting dsRNA and exposed to cold and warm temperatures was also conducted. Changes in survival rates were observed for the latter condition in dsRNA- versus saline-injected insects. These results showcase the differential expression of select targets involved in small noncoding RNA homeostasis and provide leads for the subsequent assessment of their involvement during stress response in L. decemlineata using RNAi-based approaches.

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confidence: 99%

Exposure to Temperature and Insecticides Modulates the Expression of Small Noncoding RNA-Associated Transcripts in the Colorado Potato Beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae)

Youssef

Ouédraogo

Bastarache

et al. 2022

Journal of Insect Science

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“…CPB is notorious for its high adaptive capacity in agroecosystems, especially for its polygenic insecticide resistance (Alyokhin et al, 2008; Clements et al, 2021; Crossley et al, 2017) and environmental tolerance (Hiiesaar et al, 2014). Insects in general seem to exhibit a diversity of cold‐tolerance mechanisms governed by numerous genes (Overgaard & MacMillan, 2017).…”

Section: Discussionmentioning

confidence: 99%

Polygenic adaptation contributes to the invasive success of the Colorado potato beetle

et al. 2022

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Invasive species often experience extreme demographic reductions.A common but counterintuitive phenomenon, the so-called "genetic paradox" of invasion, is that many species still successfully adapt to novel conditions despite heavy reductions in standing genetic variation and effective population size (Allendorf & Lundquist, 2003).Numerous empirical standpoints have emerged to explain this paradox, such as genetic admixture from intra- (Errbii et al., 2021;Kolbe et al., 2004) or interspecies populations (Petit et al., 2004;Wang et al., 2020), activation of transposable elements (Schrader et al., 2014;Stapley et al., 2015), and balancing selection which tends to preserve heterozygosity and prevent the loss of minor alleles (Gloag et al., 2017;Stern & Lee, 2020). However, contingencies and counterexamples prevent coalescence into general rules valid across taxa. Indeed, scepticism has been raised regarding the

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Targeted RNA sequencing reveals differential patterns of transcript expression in geographically discrete, insecticide resistant populations of Leptinotarsa decemlineata

Cited by 2 publications

References 24 publications

Exposure to Temperature and Insecticides Modulates the Expression of Small Noncoding RNA-Associated Transcripts in the Colorado Potato Beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae)

Exposure to Temperature and Insecticides Modulates the Expression of Small Noncoding RNA-Associated Transcripts in the Colorado Potato Beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae)

Polygenic adaptation contributes to the invasive success of the Colorado potato beetle

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