Targeting amino acids metabolic profile to identify novel metabolic characteristics in atrial fibrillation

Abstract

Atrial fibrillation (AF) is the most common cardiac arrhythmia whose incidence is on the rise globally. However, the pathophysiologic mechanism of AF remains poorly understood and there has been a lack of circulatory markers to diagnose and predict prognosis of AF. In the present study, by measuring metabolic profile and analyzing plasma amino acid levels in AF patients, we sought to determine whether amino acid metabolism was correlated to the occurrence of AF. Consecutive patients admitted to hospital for AF were enrolled. Plasma samples were obtained after overnight fast and a profile of 61 amino acids was then measured using gas chromatography/mass spectrometry (GC/MS). Twenty-three AF and thirty-seven control patients were enrolled in the study. A number of plasma amino acids were altered in AF, which showed significant prediction value for AF. Intriguingly, circulating 4-hydroxypyrrolidine-2-carboxylic was gradually lowered with the persistence of AF. Plasma amino acid levels were more strongly correlated with each other in AF as compared with control. By utilizing non-target metabolic profile surveys, we have found a number of altered amino acids, which exhibit diagnostic value for AF. Enhanced amino acids correlation network further identified AF as a metabolism disorder.

Serum metabolite profiles as potential biochemical markers in young adults with community-acquired pneumonia cured by moxifloxacin therapy

“…All subjects were enrolled from the respiratory and critical care department of the First Affiliated Hospital of Xi'an Jiaotong University, China, from September 2017 to September 2018. Written informed consent was obtained from all subjects according to the Declaration of Helsinki, and the study was approved by the ethics committee of Xi'an Jiaotong University [11][12][13] . We included a cohort of 13 young adults and 36 healthy controls between the ages of 18 and 30 years old.…”

“…The patients were treated on an inpatient basis. Demographic and biochemical information was obtained as previously described [12][13][14] .…”

Serum Lipids Profiling Perturbance in Patients with Stable Ischemic Heart Disease and Ischemic Cardiomyopathy

“…Demographic and biochemical information was obtained as previously described [13][14][15] Serum sample preparation Serum samples were collected from IHD, ICM and control patients after coronary angiography. Venous blood was withdrawn the next morning after overnight fast and immediately centrifuged at 3000 rpm for 10min at 4°C.…”

“…Venous blood was withdrawn the next morning after overnight fast and immediately centrifuged at 3000 rpm for 10min at 4°C. Serum was separated and stored at −80°C and aliquots were thawed for further processing as previously described [13,15].…”

Serum Lipids Profiling Perturbances in Patients with Stable Ischemic Heart Disease and Ischemic Cardiomyopathy

“…Venous blood was withdrawn the next morning after overnight fast and immediately centrifuged at 3000 rpm for 10min at 4°C. Serum was separated and stored at −80°C and aliquots were thawed for further processing as previously described[13,15]. The lipidomics data was processed by the software PeakView1.2 for qualitative analysis and MultiQuant2.1 for quantitative analysis.Statistical analysisData were normalized using MetaboAnalyst before analyses as previously discribed[16][17][18][19][20][21][22][23][24][25] (Supplementary Figure S1 andS2).…”