Targeting and Uptake of Multilayered Particles to Colorectal Cancer Cells

Abstract

Core/shell particles and capsules formed by a layer‐by‐layer technique are biofunctionalized with a humanized A33 monoclonal antibody (huA33 mAb) that binds to the A33 antigen present on colorectal cancer cells (see figure). Targeting to a colorectal cancer cell line shows selective binding and internalization of particles.

Active multilayered capsules for in vivo bone formation

“…The versatility of the LbL technique allows for facile adaptation to a colloidal system-the multilayer is simply formed on a spherical substrate, which may be decomposed to form polymeric capsules with shells of nanosized thickness (34,35). Proteins including antibodies have been adsorbed onto the surface of LbL-assembled capsules for targeting and sensing applications (36,37). Drugs, nucleic acid, and proteins have been encapsulated within the capsule core and, more recently, active peptides have been embedded within the polymer layers of the capsule shell (38)(39)(40).…”

Optofluidic vortex arrays generated by graphene oxide for tweezers, motors and self-assembly

“…36 We also demonstrated the assembly based on soft materials such as phospholipid membranes, immiscible droplets and two-phase systems. Polystyrene droplets could collide together on the vortex center, fuse into an elongated patch and subsequently fold into one sphere (Figure 5c), showing a potentially strategy for multiple-components coalescence.…”

Fluorescent Dyes Used in Polymer Carriers as Imaging Agents in Anticancer Therapy

“…The pre-condensation of pDNA with peptides improves lipid-based delivery systems and transfection efficiencies [326]. At last, by using LbL method Caruso et al [284][285][286][287][288] synthesized redoxactive polymeric microcapsules with the cross-linked shell containing disulfide bonds for the delivery of a siRNA targeting to PC-3 prostate cancer cells. The polycation-polylysine (PLL) for a siRNA complexation was infiltrated into the pores of nanoporous silica particles prior to an assembly of layer from the negatively charged poly(methacrylic acid) with thiol-modified groups (PMASH).…”

“…Such capsules sensitive to external conditions e.g., magnetic field should contain magnetic particles [281] or they have special receptor molecules like antibodies (Ab) attached to the outer side of the shell [282]. The Ab can be adsorb by electrostatic interactions [283] or covalently liked to surfaces [284][285][286][287][288]. For uncharged materials like poly(ethylene glycol) (PEG) or poly(N-vinylpyrrolidone) (PVPON) covalent attachment of the Ab is required since electrostatic attachment is not effective for lowfouling materials because they inhibit nonspecific protein binding.…”