Targeting angiogenic growth factors using therapeutic glycosaminoglycans on doppel-expressing endothelial cells for blocking angiogenic signaling in cancer

Choi, Jeong Uk; Zhang, Xiaojun; Hasan, Md. Mahedi; Karim, Mazharul; Chung, Soo Kyo; Alam, Fakhrul; Alqahtani, Faleh; Reddy, Sireesha; Kim, In‐San; Al-Hilal, Taslim A.; Byun, Youngro

doi:10.1016/j.biomaterials.2022.121423

Cited by 3 publications

(3 citation statements)

References 53 publications

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“…This showed another change in the SPR sensorgram around 500 s (Figure B). This change in the sensorgram was due to the binding of the −NH 2 group of anti-Lep with the EDC-NHS . The sensorgram showed a declining trend as over time the anti-Lep dissociated from the EDC-NHS/Fe:Ni(1:1)/C_Au sensor surface .…”

Section: Resultsmentioning

confidence: 96%

“…This change in the sensorgram was due to the binding of the −NH 2 group of anti-Lep with the EDC-NHS. 38 The sensorgram showed a declining trend as over time the anti-Lep dissociated from the EDC-NHS/Fe:Ni(1:1)/C_Au sensor surface. 37 However, the sensorgram showed minimal change in its response over a time period of 500 s. This clearly indicated that the anti-Lep and EDC-NHS/Fe:Ni(1:1)/C interaction was steady.…”

Section: Optimization and Stabilitymentioning

confidence: 91%

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Detection of Leptin Using Electrocatalyst Mediated Impedimetric Sensing

Islam

Hassan

Afrin

et al. 2022

ACS Biomater. Sci. Eng.

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Obesity is a complex disorder associated with immense health consequences including high risk of cardiovascular diseases, diabetes, and cancer. Abnormality in the thyroid gland, genetics, less physical activity, uptake of excessive diet, and leptin resistance are critical factors in the development of obesity. To determine the treatment strategy, understanding the pathophysiology of obesity is crucial. For instances, leptin resistance mediated obesity defined by the presence of excessive leptin hormone (Lep) in the systemic circulation is very common in diet induced obesity. Therefore, our hypothesis is that quantitative measurement of Lep from blood can help to identify individuals with Lep resistant mediated obesity and thereby guide toward a proper treatment strategy. In this work, we aim to utilize an electrochemical immunosensing platform for diagnosis of obesity by measuring the Lep content in systemic circulation. A porous carbon confined FeNi bimetallic system was synthesized with three different ratios of Fe and Ni ions using high temperature pyrolysis technique. The suitability of the sensor for detecting Lep was studied using both CV and EIS techniques. The limit of detection (LOD) for GCE was recorded as 157.4 fg/mL with a wide linear concentration range of 500 fg/mL to 80 ng/mL, while for SPCE the LOD was 184.9 fg/mL with a linear range of 500 fg/mL to 50 ng/mL. Finally, the feasibility and applicability of the sensor for Lep detection was tested with serum collected from high fat diet induced obese rats. The selectivity, sensitivity, storage, and experimental stability and reproducibility tests showed potential for this biosensor platform as a point-of-care Lep detection device.

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Section: Resultsmentioning

confidence: 96%

Section: Optimization and Stabilitymentioning

confidence: 91%

Detection of Leptin Using Electrocatalyst Mediated Impedimetric Sensing

Islam

Hassan

Afrin

et al. 2022

ACS Biomater. Sci. Eng.

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“…In a BALB/c animal model containing ct26, doxorubicin coupled with heparin, which produced ROS by cytotoxicity, exhibited inhibitory effects on tumor growth and metastasis. Jeong Uk Choi et al synthesized LMWH-bile acid conjugates (LMWH with four dimeric dextrocholic acid molecules), which selectively target the tumor endothelial cell markers, doppel, and the doppel/VEGFR2 axis and capture VEGF, with the trio forming an intermediate complex that further inhibits the process of tumor angiogenesis [ 104 ].…”

Section: Various Heparin Conjugates With Non-coagulant Effectsmentioning

confidence: 99%

Non-Anticoagulant Activities of Low Molecular Weight Heparins—A Review

Feng,

Wang,

Zhou

et al. 2023

Pharmaceuticals

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Low molecular weight heparins (LMWHs) are derived from heparin through chemical or enzymatic cleavage with an average molecular weight (Mw) of 2000–8000 Da. They exhibit more selective activities and advantages over heparin, causing fewer side effects, such as bleeding and heparin-induced thrombocytopenia. Due to different preparation methods, LMWHs have diverse structures and extensive biological activities. In this review, we describe the basic preparation methods in this field and compare the main principles and advantages of these specific methods in detail. Importantly, we focus on the non-anticoagulant pharmacological effects of LMWHs and their conjugates, such as preventing glycocalyx shedding, anti-inflammatory, antiviral infection, anti-fibrosis, inhibiting angiogenesis, inhibiting cell adhesion and improving endothelial function. LMWHs are effective in various diseases at the animal level, including cancer, some viral diseases, fibrotic diseases, and obstetric diseases. Finally, we briefly summarize their usage and potential applications in the clinic to promote the development and utilization of LMWHs.

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