Targeting Antibodies to Carbon Nanotube Field Effect Transistors by Pyrene Hydrazide Modification of Heavy Chain Carbohydrates

Stefansson, Steingrimur; Kwon, Hena H.; Ahn, Saeyoung Nate

doi:10.1155/2012/490175

Cited by 9 publications

(13 citation statements)

References 39 publications

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“…The SWNT networks are first incubated for 1 h with a mixture of 1-Pyrenebutyric acid (Sigma Aldrich, St. Louis, MO, USA) and 1-Pyrenebutyric acid N-hydroxysuccinimide ester (PBASE) (Sigma Aldrich, St. Louis, MO, USA) at a 3:1 molar ratio into 1 mL of dimethylformamide (DMF). This resulted in the pyrene moieties non-covalently attaching to the SWNT by π-π stacking while exposing the N-hydroxysuccinimide ester for covalent attachment with anti-NS1 antibody with an amide bond [ 34 , 35 ]. Chips were washed with DMF, followed by drying with N 2 gas.…”

Section: Methodsmentioning

confidence: 99%

Salivary Detection of Dengue Virus NS1 Protein with a Label-Free Immunosensor for Early Dengue Diagnosis

Wasik

Mulchandani

Yates

2018

Sensors

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Dengue virus (DENV) is a highly pathogenic, arthropod-borne virus transmitted between people by Aedes mosquitoes. Despite efforts to prevent global spread, the potential for DENV epidemics is increasing world-wide. Annually, 3.6 billion people are at risk of infection. With no licensed vaccine, early diagnosis of dengue infection is critical for clinical management and patient survival. Detection of DENV non-structural protein 1 (NS1) is a clinically accepted biomarker for the early detection of DENV infection. Unfortunately, virtually all of the laboratory and commercial DENV NS1 diagnostic methods require a blood draw for sample analysis, limiting point-of-care diagnostics and decreases patient willingness. Alternatively, NS1 in human saliva has been identified for the potential early diagnosis of DENV infection. The collection of saliva is simple, non-invasive, painless, and inexpensive, even by minimally trained personnel. In this study, we present a label-free chemiresistive immunosensor for the detection of the DENV NS1 protein utilizing a network of single-walled carbon nanotubes functionalized with anti-dengue NS1 monoclonal antibodies. NS1 was successfully detected in adulterated artificial human saliva over the range of clinically relevant concentrations with high sensitivity and selectivity. It has potential application in clinical diagnosis and the ease of collection allows for self-testing, even within the home.

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Section: Methodsmentioning

confidence: 99%

Salivary Detection of Dengue Virus NS1 Protein with a Label-Free Immunosensor for Early Dengue Diagnosis

Wasik

Mulchandani

Yates

2018

Sensors

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“…5(a)]. 78,136 Covalent conjugation provides a stronger and more durable bond, which can interfere with the CNT electronic structure. Indeed, the procedure of generating covalent bonds on the CNT surfaces (e.g., the employment of a strong acid) can generate defects in the nanotube backbone.…”

Section: A Functionalization Methodsmentioning

confidence: 99%

“…In addition to their large employment, the main drawback is related to their bulky size that can lead to detection of the analyte outside the Debye length. 136 The Debye length issue can be addressed through the use of aptamers, which are single-stranded oligonucleotides, synthesized in vitro using the systematic evolution of ligands by exponential enrichment (SELEX) process. 147,148 By varying the primary oligonucleotide sequences, high selectivity can be achieved, and this can be done for a wide range of analytes from proteins to small molecules.…”

Section: B Bio-recognition Elementsmentioning

confidence: 99%

Electrolyte-gated carbon nanotube field-effect transistor-based biosensors: Principles and applications

Shkodra

Petrelli

Angeli

et al. 2021

Applied Physics Reviews

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Nowadays, there is a high demand for sensitive and selective real-time analytical methods suitable for a wide range of applications, from personalized telemedicine, drug discovery, food safety, and quality control, to defense, security, as well as environmental monitoring. Biosensors are analytical devices able to detect bio-chemical analytes (e.g., neurotransmitters, cancer biomarkers, bio-molecules, and ions), through the combination of a bio-recognition element and a bio-transduction device. The use of customized bio-recognition elements such as enzymes, antibodies, aptamers, and ion-selective membranes facilitates achieving high selectivity. Among the different bio-transduction devices currently available, electrolyte-gated field-effect transistors, in which the dielectric is represented by an ionic liquid buffer solution containing the targeted analyte, are gaining increasing attention. Indeed, these biotransduction devices are characterized by superior electronic properties and intrinsic signal amplification that allow the detection of a wide rangeofbiomolecules with high sensitivity (down to pM concentration). A promising semiconducting material for bio-transduction devices is represented by carbon nanotubes, due to their unique electrical properties, nanosize, bio-compatibility, and their simple low-cost processability. This work provides acomprehensive and critical review of electrolyte-gated carbon nanotube field-effect transistor-based biosensors. First, an introduction to these bio-sensing devices is given. Next, the device configurations and operating principles are presented, and the most used materials and processes are reviewed with a particular focus on carbon nanotubes as the active material. Subsequently, different functionalization strategies reported in the literature, based on enzymes, antibodies, aptamers, and ion-selective membranes, are analyzed critically. Finally, present issues and challenges faced in the area are investigated,theconclusions are drawn, and a perspective outlook over the field of bio-sensing technologies, in general, is provided.

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“…Previously we have shown that SWCNT-FET can be utilized to measure many biological molecules including IGF-1 [43], glycated human serum albumin [44] and hemorrhagic E coli [45]. In the present study we examine the interactions of A40 and Aβ42 peptides with SWCNTs immobilized on silica wafers.…”

Section: Introductionmentioning

confidence: 97%

Specific Binding of Alzheimer's Aβ Peptide Fibrils to Single-Walled Carbon Nanotubes

Stefansson¹,

Knight²,

Ahn³

2012

Nanomaterials and Nanotechnology

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Amyloids constitute a class of protein and protein fragments believed to be involved in the pathologies associated with Alzheimer's, Parkinson's and CreutzfeldtJakob diseases. These proteins can self-assemble into unique fibrillar structures that are resistant to normal protein degradation. Interesting recent developments in the study of amyloid fibrils demonstrate that they bind carbon allotropes. In this study, using single-walled carbon nanotube fieldeffect transistors (SWCNT-FETs), we show that the fibrillar form of Alzheimer's amyloid β (1-40) and (1-42) peptides specifically bind non-functionalized SWCNT in a saturable manner. Both peptides exhibited near identical binding curves with half-maximal binding concentrations of approximately 12 g/ml. Binding of the peptides to SWCNTs was diminished by including dimethyl sulphoxide (DMSO) at concentrations that inhibits fibril formation. Lastly, a monoclonal antibody (BAM-10), which binds to the N-terminal region of Alzheimer's amyloid fibrils, recognizes the amyloid peptides adhering to SWCNTs in the absence of DMSO, but not in the presence of 75% DMSO. Taken together, these results suggest that the fibrillar form of the Alzheimer's amyloid peptides are specifically binding to SWCNTs.

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Targeting Antibodies to Carbon Nanotube Field Effect Transistors by Pyrene Hydrazide Modification of Heavy Chain Carbohydrates

Cited by 9 publications

References 39 publications

Salivary Detection of Dengue Virus NS1 Protein with a Label-Free Immunosensor for Early Dengue Diagnosis

Salivary Detection of Dengue Virus NS1 Protein with a Label-Free Immunosensor for Early Dengue Diagnosis

Electrolyte-gated carbon nanotube field-effect transistor-based biosensors: Principles and applications

Specific Binding of Alzheimer's Aβ Peptide Fibrils to Single-Walled Carbon Nanotubes

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